Pierce™ 660 nm 蛋白检测试剂
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Pierce™ 660 nm 蛋白检测试剂
Thermo Scientific™

Pierce™ 660 nm 蛋白检测试剂

Pierce 660-nm 蛋白检测试剂是一种即用型去污剂和还原剂兼容试剂,能够快速测定总蛋白浓度。该检测与基于考马斯的 Bradford 检测相比更具有线性,并且与更高浓度的大多数去污剂、还原剂和其他常用试剂兼容。请参见所有可用蛋白检测›660-nm 蛋白检测试剂盒的特点包括:•兼容性—与其他基于染料的检测相比,与更广泛的去污剂和还原剂配合使用•即用型—使用简单混合-读取方案的单一试剂;没有要制备的工作试剂•线性—产生比使用 Bradford了解更多信息
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货号数量
22660750 mL
货号 22660
价格(CNY)
2,048.00
飞享价
Ends: 31-Dec-2025
2,793.00
共减 745.00 (27%)
Each
添加至购物车
数量:
750 mL
请求批量或定制报价
价格(CNY)
2,048.00
飞享价
Ends: 31-Dec-2025
2,793.00
共减 745.00 (27%)
Each
添加至购物车
Pierce 660-nm 蛋白检测试剂是一种即用型去污剂和还原剂兼容试剂,能够快速测定总蛋白浓度。该检测与基于考马斯的 Bradford 检测相比更具有线性,并且与更高浓度的大多数去污剂、还原剂和其他常用试剂兼容。

请参见所有可用蛋白检测›

660-nm 蛋白检测试剂盒的特点包括:
兼容性—与其他基于染料的检测相比,与更广泛的去污剂和还原剂配合使用
即用型—使用简单混合-读取方案的单一试剂;没有要制备的工作试剂
线性—产生比使用 Bradford 检测方法更具线性的标准曲线
检测形式—可在试管或微孔板中进行检测
样品体积—微孔板仅需要 10 μL,检测管程序需要 100 μL
储存—室温储存

辅助离子型去污剂兼容性试剂 (IDCR) 提供了更广泛的去污剂兼容性,使其成为唯一一种适用于含 Laemmli SDS 样品缓冲液与溴酚蓝的样品的蛋白检测方法。尽管 Pierce 660-nm 蛋白检测产生的蛋白间变异性比其他检测法(如 BCA 蛋白检测法)高 (37%),但更简单的单试剂形式和更广泛的物质兼容性使 Pierce 660-nm 检测对于许多常规应用来说更方便。Pierce 660-nm 蛋白检测可以试管或微孔板形式进行。

Pierce 660-nm 检测如何检测蛋白
Pierce 660 蛋白检测基于酸性条件下具有专利技术的染料金属复合物与蛋白的结合,导致染料吸收最大值(在 660 nm 下测得)的转变。该染料-金属复合物为红褐色,在与蛋白结合后变为绿色。这种颜色的变化是由于染料在低 pH 值时的脱氢导致的,而蛋白内的带正电荷的氨基酸残基和带负电的染料-金属复合物的相互作用促进了这一过程。因此,该染料主要与蛋白中的碱性残基(例如组氨酸、精氨酸和赖氨酸)相互作用,而与酪氨酸、色氨酸和苯丙氨酸相互作用程度相对较小。

检测中产生的颜色稳定且与广泛的增加蛋白浓度成比例增加,即使在存在与 Bradford 和 BCA 蛋白检测不兼容的去污剂和还原剂的情况下也是如此。

相关产品
Pierce 660-nm 蛋白检测试剂盒(包括蛋白标准品)
Pierce 660-nm 蛋白检测试剂的离子型去污剂兼容性试剂
仅供科研使用。不可用于诊断程序。
规格
检测蛋白检测试剂
描述Pierce 660 nm 蛋白检测试剂
适用于(应用)基于溶液的检测、吸光度
适用于(设备)分光光度计、酶标仪
产品线Pierce™
产品类型蛋白定量检测
数量750 mL
特异性无靶标特异性
足够用于500 次试管检测或 5000 次微孔板检测
检测方法比色法
Unit SizeEach
内容与储存
将检测试剂置于室温下储存。

常见问题解答 (FAQ)

Can I use the Thermo Scientific 660 nm Protein Assay to quantify peptides?

The Thermo Scientific 660 nm Protein Assay can quantify peptides that are at least 2,500 Da if their compositions include amino acid residues that react with the dye-metal reagent (i.e., histidine, arginine, tyrosine, tryptophan and phenylalanine). For peptides smaller than 2,500 Da, use the Fluoraldehyde Reagent Solution (Cat. No. 26025), which detects amino groups.

Other peptide quantitation assays we offer are Thermo Scientific Pierce Quantitative Colorimetric Peptide Assay (Cat. No. 23275) and Thermo Scientific Pierce Quantitative Fluorometric Peptide Assay (Cat. No. 23290).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the linear range of the Thermo Scientific 660 nm Protein Assay?

The assay's linear range is 25-2,000 µg/mL using the test tube procedure and 50-2,000 µg/mL using the microplate procedure.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I measure the absorbance at a wavelength other than 660 nm when using the Thermo Scientific 660nm Protein Assay Kit?

If a 660nm filter is not available, the assay can be measured at wavelengths from 645 to 670nm; however, the assay linear range is 25-2,000 µg/mL and occurs only when the absorbance of the dye-protein complex is measured at 660 nm. Measuring the absorbance at wavelengths other than 660nm will result in a decrease of the assay's linear range and might increase the minimum detection level (i.e., decrease sensitivity).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What advantages does the Thermo Scientific 660nm Protein Assay have over other protein assays?

The Thermo Scientific 660 nm Protein Assay has the following advantages: • Room temperature stability of the assay reagent • A greater linear range than the coomassie-based Bradford assays • Compatibility with commonly used detergents and reducing agents • Compatibility with samples lysed in Laemmli sample buffer • Rapid mix-and-read protocol

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What formats are available for the Thermo Scientific 660 nm Protein Assay?

We offer two formats: The complete kit (Cat. No. 22662) contains sufficient reagents for 300 test tube or 3,000 microplate assays and a set of pre-diluted BSA standards with concentrations from 125 to 2,000 µg/mL. The assay reagent (750 ml, Cat. No. 22660) is sufficient for 500 test tube or 5,000 microplate assays.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.