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The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy.
Kits are available with or without Pierce Dilution-Free BSA Protein Standards, which are a set of seven pre-diluted BSA standards, packaged in a multichannel pipette-friendly tubestrip. The tubestrip includes a single empty tube that enables users to add their own sample buffer for the purpose of blank subtraction.
浓度 | BSA: 2 mg/mL |
描述 | Pierce BCA 蛋白检测试剂盒 |
检测 | BCA 蛋白定量: |
检测方法 | 比色法 |
产品线 | Pierce™ |
产品类型 | 蛋白定量检测 |
特异性 | 无靶标特异性 |
足够用于 | 500 次试管检测或 5000 次微孔板检测 |
适用于(应用) | 基于溶液的检测、吸光度 |
适用于(设备) | 分光光度计、酶标仪 |
套装内容 | 10 x 1 mL glass ampules |
数量 | 1 L 试剂盒 |
Unit Size | 1 L |
浓度 | BSA: 2 mg/mL |
描述 | Pierce BCA Protein Assay Kit, 100 mL |
检测 | BCA Assay |
检测方法 | Colorimetric |
产品线 | Pierce™ |
产品类型 | Protein Quantitation Assay |
特异性 | Not Target-Specific |
足够用于 | 50 Tube Assays or 500 Microplate Assays |
适用于(应用) | Solution-based Detection, Absorbance |
适用于(设备) | Spectrophotometer, Microplate Reader |
套装内容 | 2 mL vial |
数量 | 1 Kit (100 mL) |
Unit Size | 100 mL |
浓度 | BSA: 2 mg/mL |
描述 | Pierce BCA 蛋白检测试剂盒 |
检测 | BCA 蛋白定量: |
检测方法 | 比色法 |
产品线 | Pierce™ |
产品类型 | 蛋白定量检测 |
特异性 | 无靶标特异性 |
足够用于 | 250 次试管检测或 2500 次微孔板检测 |
适用于(应用) | 基于溶液的检测、吸光度 |
适用于(设备) | 分光光度计、酶标仪 |
套装内容 | 10 x 1 mL glass ampules |
数量 | 500 mL 试剂盒 |
Unit Size | 500 mL |
浓度 | BSA: 0.125, 0.25, 0.5, 0.75, 1, 1.5, and 2 mg/mL |
描述 | Pierce BCA Protein Assay Kit with Dilution-Free BSA Protein Standards |
检测 | BCA Assay |
检测方法 | Colorimetric |
产品线 | Pierce™ |
产品类型 | Protein Quantitation Assay |
特异性 | Not Target-specific |
足够用于 | 500 Tube Assays or 5000 Microplate Assays |
适用于(应用) | Solution-based Detection, Absorbance |
适用于(设备) | Spectrophotometer, Microplate Reader |
套装内容 | 4 x 1 mL 8-channel tubestrip, prediluted |
数量 | 1 Kit (1 L) |
Unit Size | 1 L |
浓度 | BSA: 0.125, 0.25, 0.5, 0.75, 1, 1.5, and 2 mg/mL |
描述 | Pierce BCA Protein Assay Kit with Dilution-Free BSA Protein Standards |
检测 | BCA Assay |
检测方法 | Colorimetric |
产品线 | Pierce™ |
产品类型 | Protein Quantitation Assay |
特异性 | Not Target-specific |
足够用于 | 250 Tube Assays or 2500 Microplate Assays |
适用于(应用) | Solution-based Detection, Absorbance |
适用于(设备) | Spectrophotometer, Microplate Reader |
套装内容 | 2 x 1 mL 8-channel tubestrip, prediluted |
数量 | 1 Kit (500 mL) |
Unit Size | 500 mL |
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23225 | 1 L | Pierce BCA 蛋白检测试剂盒 | BSA: 2 mg/mL | 10 x 1 mL glass ampules | 价格 3,037.00 您的价格 | *** | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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A65453 | 100 mL | Pierce BCA Protein Assay Kit, 100 mL | BSA: 2 mg/mL | 2 mL vial | 价格 499.00 飞享价 350.00 (结束31-Dec-2024) 您的价格 | *** | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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23227 | 500 mL | Pierce BCA 蛋白检测试剂盒 | BSA: 2 mg/mL | 10 x 1 mL glass ampules | 价格 1,887.00 您的价格 | *** | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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A55864 | 1 L | Pierce BCA Protein Assay Kit with Dilution-Free BSA Protein Standards | BSA: 0.125, 0.25, 0.5, 0.75, 1, 1.5, and 2 mg/mL | 4 x 1 mL 8-channel tubestrip, prediluted | 价格 3,052.00 飞享价 2,136.00 (结束31-Dec-2024) 您的价格 | *** | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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A55865 | 500 mL | Pierce BCA Protein Assay Kit with Dilution-Free BSA Protein Standards | BSA: 0.125, 0.25, 0.5, 0.75, 1, 1.5, and 2 mg/mL | 2 x 1 mL 8-channel tubestrip, prediluted | 价格 1,895.00 飞享价 1,327.00 (结束31-Dec-2024) 您的价格 | *** | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The Pierce BCA Protein Assay Kit is a high-precision, detergent-compatible protein assay for determination of protein concentration. Pierce BCA reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA assay can be used to assess yields in whole cell lysates, affinity-column fractions, purified proteins samples, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA assay is affected much less by protein compositional differences, providing greater concentration accuracy.
Kit options are available that include Pierce Dilution-Free BSA Protein Standards, which are a set of seven pre-diluted BSA standards, packaged in a multichannel pipette-friendly tubestrip. The tubestrip includes a single empty tube that enables users to add their own sample buffer for the purpose of blank subtraction.
Features of the BCA Protein Assay Kit include:
How the assay works
The BCA Protein Assay combines the well-known reduction of Cu2+ to Cu1+ by protein in an alkaline medium with the highly sensitive and selective colorimetric detection of the cuprous cation (Cu1+) by bicinchoninic acid (BCA). The first step is the chelation of copper with protein in an alkaline environment to form a light blue complex. In this reaction, known as the biuret reaction, peptides containing three or more amino acid residues form a colored chelate complex with cupric ions in an alkaline environment containing sodium potassium tartrate.
In the second step of the color development reaction, BCA reacts with the reduced (cuprous) cation that was formed in step one. The intense purple-colored reaction product results from the chelation of two molecules of BCA with one cuprous ion. The BCA/copper complex is water soluble and exhibits a strong linear absorbance at 562 nm with increasing protein concentrations. The complex is approximately 100 times more sensitive (lower limit of detection) than the pale blue color of the first reaction.
The reaction is strongly influenced by four amino acid residues (cysteine, cystine, tyrosine, and tryptophan) in the amino acid sequence of the protein. However, unlike Coomassie dye-binding methods (i.e., Bradford), the universal peptide backbone also contributes to color formation, helping to minimize variability caused by protein compositional differences.