Pierce™ ECL Western 印迹底物
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Pierce™ ECL Western 印迹底物
Thermo Scientific™

Pierce™ ECL Western 印迹底物

Thermo Scientific Pierce ECL Western 印迹底物是一种用于增强化学发光 (ECL) 的高性价比入门级辣根过氧化物酶 (HRP) 底物,可直接取代成本较高的产品,且无需重新优化条件了解更多信息
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货号数量
32106500 mL
3210950 mL
32209250 mL
32106TS
又称 32106
货号 32106
价格(CNY)
3,231.00
Each
添加至购物车
数量:
500 mL
请求批量或定制报价
价格(CNY)
3,231.00
Each
添加至购物车
Thermo Scientific Pierce ECL Western 印迹底物是一种用于增强化学发光 (ECL) 的高性价比入门级辣根过氧化物酶 (HRP) 底物,可直接取代成本较高的产品,且无需重新优化条件。

Pierce ECL Plus Western 免疫印迹底物的特性包括:
灵敏度:低皮克级
稳定性:4°C 下,工作溶液可保持稳定 1 小时,试剂盒可保持稳定 1 年
兼容性:硝酸纤维素膜和 PVDF 膜
信号持续时间:1 至 2 小时
推荐的一抗浓度:1:1,000–1:5,000 稀释度 (0.2–10 µg/mL)
推荐的二抗浓度:1:1,000–1:15,000 稀释度 (0.07–1.0 µg/mL)

比较并详细了解 Western 免疫印迹化学发光底物 ›
查看所有可用的 HRP 底物 ›

Pierce ECL Western 印迹底物在 HRP 酶活性检测方面能够提供与其他标准 ECL 底物同等的可靠性和性能。它能够检测皮克量的抗原,并支持使用照相或其他成像方法轻松检测 HRP。

由于鲁米诺和过氧化物试剂配方与其他市售底物产品相同,用户可以直接切换至 Pierce ECL,无需优化探测条件或孵育方案。Pierce ECL Western 印迹底物与 GE Healthcare 原始 Amersham ECL Western 印迹检测试剂具有相同性能。

仅供科研使用。不可用于诊断程序。
规格
偶联物HRP
描述Pierce ECL Western 印迹底物
套装内容鲁米诺/增强剂,250 mL, 稳定过氧化物缓冲液,250 mL
建议抗体浓度2°:1:1-15K (0.07-1μg/mL),1°:1:1K (0.2- 10μg/mL)
灵敏度低皮克水平
信号持续时间1 - 2 小时
足够用于5000 sq. cm
检测方法化学发光
适用于(应用)蛋白质免疫印迹
形式液体
膜兼容性硝酸纤维素、PVDF
产品线Pierce™
产品类型底物
数量500 mL
基质辣根过氧化物酶
Unit SizeEach
内容与储存
接收后,在 2-8°C 下储存。产品在环境温度下运输。

常见问题解答 (FAQ)

SuperSignal West Femto化学发光底物比其他增强型化学发光(ECL)底物灵敏度高多少?

SuperSignal West Femto化学发光底物的灵敏度比GE Healthcare ECL底物或Thermo Scientific ECL底物(货号32106)高25-50倍。SuperSignal West Femto化学发光底物的灵敏度也显著高于碱性磷酸酶的化学发光底物。

SuperSignal West Dura化学发光底物比其他增强型化学发光(ECL)底物灵敏度高多少?

SuperSignal West Dura化学发光底物的灵敏度比GE Healthcare ECL底物或Thermo Scientific ECL底物(货号32106)高10倍。SuperSignal West Dura化学发光底物的灵敏度也显著高于碱性磷酸酶的化学发光底物。

How much more sensitive is SuperSignal West Femto Maximum Sensitivity Substrate than the other enhanced chemiluminescent (ECL) substrates?

SuperSignal West Femto Maximum Sensitivity Substrate is about 25- to 50-fold more sensitive than GE Healthcare ECL Substrate or Thermo Scientific ECL Substrate (Cat. No. 32106). SuperSignal West Femto Maximum Sensitivity Substrate is also significantly more sensitive than available chemiluminescent substrates for alkaline phosphatase. Please also see Tech Tip # 32 Guide to Enzyme Substrates for Western Blotting (https://tools.thermofisher.com/content/sfs/brochures/TR0032-Substrates-Western.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How much more sensitive is SuperSignal West Dura Extended Duration Substrate than the other enhanced chemiluminescent (ECL) substrates?

SuperSignal West Dura Extended Duration Substrate is about 10-fold more sensitive than GE Healthcare ECL Substrate or Thermo Scientific ECL Substrate (Cat. No. 32106). SuperSignal West Dura Extended Duration Substrate is also significantly more sensitive than available chemiluminescent substrates for alkaline phosphatase. Please also see Tech Tip: Guide to Enzyme Substrates for Western Blotting (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0032-Substrates-Western.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How can I increase the sensitivity of my Western blot?

Consider transferring to a different membrane or using a different detection method. We have observed increased sensitivity when using PVDF membranes in place of nitrocellulose. On PVDF membranes, using as little as 1 µg of total rat brain protein, PKC can be detected with alkaline phosphatase-mediated chromogenic detection in some cases using affinity-purified antibodies at a concentration of 0.5 µg/mL. Detection sensitivity can also be increased by using chemiluminescent detection, especially when using a SuperSignal West enhanced chemiluminescence subtrate (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-detection-reagents/detection-technologies-western-blotting/chemiluminescent-western-blot-detection/supersignal-chemiluminescent-substrates.html) such as SuperSignal West Pico PLUS, SuperSignal West Dura, or SuperSignal West Femto. The secondary antibody should be used as recommend by the manufacturer and optimized as needed.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.