SuperSignal West Femto化学发光底物比其他增强型化学发光(ECL)底物灵敏度高多少?
SuperSignal West Femto化学发光底物的灵敏度比GE Healthcare ECL底物或Thermo Scientific ECL底物(货号32106)高25-50倍。SuperSignal West Femto化学发光底物的灵敏度也显著高于碱性磷酸酶的化学发光底物。
SuperSignal West Dura化学发光底物比其他增强型化学发光(ECL)底物灵敏度高多少?
SuperSignal West Dura化学发光底物的灵敏度比GE Healthcare ECL底物或Thermo Scientific ECL底物(货号32106)高10倍。SuperSignal West Dura化学发光底物的灵敏度也显著高于碱性磷酸酶的化学发光底物。
How much more sensitive is SuperSignal West Femto Maximum Sensitivity Substrate than the other enhanced chemiluminescent (ECL) substrates?
SuperSignal West Femto Maximum Sensitivity Substrate is about 25- to 50-fold more sensitive than GE Healthcare ECL Substrate or Thermo Scientific ECL Substrate (Cat. No. 32106). SuperSignal West Femto Maximum Sensitivity Substrate is also significantly more sensitive than available chemiluminescent substrates for alkaline phosphatase. Please also see Tech Tip # 32 Guide to Enzyme Substrates for Western Blotting (https://tools.thermofisher.com/content/sfs/brochures/TR0032-Substrates-Western.pdf).
How much more sensitive is SuperSignal West Dura Extended Duration Substrate than the other enhanced chemiluminescent (ECL) substrates?
SuperSignal West Dura Extended Duration Substrate is about 10-fold more sensitive than GE Healthcare ECL Substrate or Thermo Scientific ECL Substrate (Cat. No. 32106). SuperSignal West Dura Extended Duration Substrate is also significantly more sensitive than available chemiluminescent substrates for alkaline phosphatase. Please also see Tech Tip: Guide to Enzyme Substrates for Western Blotting (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0032-Substrates-Western.pdf).
How can I increase the sensitivity of my Western blot?
Consider transferring to a different membrane or using a different detection method. We have observed increased sensitivity when using PVDF membranes in place of nitrocellulose. On PVDF membranes, using as little as 1 µg of total rat brain protein, PKC can be detected with alkaline phosphatase-mediated chromogenic detection in some cases using affinity-purified antibodies at a concentration of 0.5 µg/mL. Detection sensitivity can also be increased by using chemiluminescent detection, especially when using a SuperSignal West enhanced chemiluminescence subtrate (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-detection-reagents/detection-technologies-western-blotting/chemiluminescent-western-blot-detection/supersignal-chemiluminescent-substrates.html) such as SuperSignal West Pico PLUS, SuperSignal West Dura, or SuperSignal West Femto. The secondary antibody should be used as recommend by the manufacturer and optimized as needed.