Invitrogen

JPH2 Polyclonal Antibody

Name: JPH2 Polyclonal Antibody
Brand: Invitrogen
SKU: 40-5300

高级验证

17个已发表图片

23 篇参考文献

查看其他11个JPH2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

JPH2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 23

JPH2 Antibody (40-5300) in IHC (P)

Immunohistochemistry analysis of Junctophilin-2 showing staining in the cytoplasm and membrane of paraffin-embedded mouse small intestine tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for... View More

JPH2 Antibody in Immunohistochemistry (IHC)

JPH2 Antibody in Immunocytochemistry (ICC/IF)

JPH2 Antibody in Immunohistochemistry (Frozen) (IHC (F))

Product Image Gallery

JPH2 Antibody — Effect of Cav-3 OE and TAC on Cav-3 and JPH-2 protein expression. (a) Left panel, exemplar western blots of Cav-3 (18 kDa) and GAPDH (37 kDa) in isolated myocyte lysates from three WT sham (lanes 1), three Cav-3 OE sham (lanes 2) and three Cav-3 OE TAC (lanes 3) mice; Right panel, densitometry analysis of Cav-3 western blots ( N = 5 animals in each group in duplicate), compared with previously published data (Bryant et al., ) showing the effect of TAC on Cav-3 expression in WT mice (left two bars). (b) Left panel, exemplar western blots of JPH-2 (95 kDa) and GAPDH (37 kDa); right panel, densitometry analysis of JPH-2 western blots ( N = 5 animals in each group in duplicate), compared with previously published data (Bryant et al., ) showing the effect of TAC on JPH-2 expression in WT mice (left two bars). The blots in the left panels are from the same gels which were stripped and re-probed for the different proteins and were therefore obtained sequentially (see Methods). Data in each group (WT or Cav-3 OE) in the right panels are expressed as a percentage of the mean of the WT data in that group. ** P < 0.01, between treatments for a given phenotype (WT or Cav-3 OE); ++ P < 0.01, +++P < 0.001 between phenotypes {TM}

Immunogen image

Immunogen Image JPH2 Polyclonal Antibody

产品信息

40-5300

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2-3 µg/mL

查看12篇已发表文章 12篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

1:20

免疫组化（冰冻） (IHC (F))

Assay-dependent

查看3篇已发表文章 3篇已发表文章

免疫细胞化学 (ICC/IF)

查看5篇已发表文章 5篇已发表文章

亲和纯化 (AP)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Mouse

已发表种属

C. elegans, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from the C-terminal region of the mouse junctophilin-2 (JPH2, JP-2, junctophilin type 2) protein, which differs from rat by one non-conservative amino acid

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533471

靶标信息

This gene encodes a protein with a leucine-rich repeat and a calponin homology domain. Polymorphism in this gene may be associated with susceptibililty to knee osteoarthritis. Alternative splicing results in multiple transcript variants encoding different isoforms.

仅用于科研。不用于诊断过程。未经明确授权不得转售。