胰岛素-转铁蛋白-硒 (ITS -G) (100X)
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胰岛素-转铁蛋白-硒 (ITS -G) (100X)
胰岛素-转铁蛋白-硒 (ITS -G) (100X)
胰岛素-转铁蛋白-硒 (ITS -G) (100X)
引用和文献 (4)
Gibco™

胰岛素-转铁蛋白-硒 (ITS -G) (100X)

使用 Gibco™ 胰岛素-转铁蛋白-硒 (ITS-G) 作为基础培养基补充剂，以减少培养细胞所需的胎牛血清 (FBS) 的量。ITS-G 溶液最初配合 RPMI了解更多信息
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货号数量
4140004510 mL
货号 41400045
价格（CNY)
1,056.00
飞享价
Ends: 27-Dec-2025
1,242.00
共减 186.00 (15%)
Each
添加至购物车
数量:
10 mL
价格（CNY)
1,056.00
飞享价
Ends: 27-Dec-2025
1,242.00
共减 186.00 (15%)
Each
添加至购物车
使用 Gibco™ 胰岛素-转铁蛋白-硒 (ITS-G) 作为基础培养基补充剂，以减少培养细胞所需的胎牛血清 (FBS) 的量。ITS-G 溶液最初配合 RPMI 1640 和最低必需培养基 (MEM) 使用，现在通常配合其他基础培养基使用，以在存在 2–4% FBS 的情况下支持多种细胞类型。

胰岛素可促进葡萄糖和氨基酸摄取、脂肪形成、细胞内运输以及蛋白和核酸的合成。转铁蛋白是铁载体，也有助于降低氧自由基和过氧化物的毒性水平。硒（以亚硒酸钠提供）是谷胱甘肽过氧化物酶和其他蛋白的一种辅因子，在培养基中用作抗氧化剂。

可提供完整配方。

cGMP 生产和质量体系
Gibco™ ITS-G 在位于纽约格兰德岛的符合 cGMP 要求的工厂内生产。该工厂是在FDA登记的医疗器械生产商，且通过ISO 13485标准认证。
仅供科研使用。不可用于诊断程序。
规格
细胞类型哺乳动物细胞
最大浓度100 X
数量10 mL
有效期18 个月
运输条件室温
形式液体
产品类型转铁蛋白
无菌无菌过滤
不加添加剂无酚红
Unit SizeEach
内容与储存
储存条件：2°C 至 8°C
运输条件：室温
有效期：自生产之日起 18 个月

常见问题解答 (FAQ)

What is the buffer composition of the various Insulin-Transferrin-Selenium (ITS) (100X) and Insulin-Transferrin-Selenium (ITS) Select (100X) supplements?

All ITS and ITS Select supplements are formulated in Earle's Balanced Salt Solution (EBSS).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Insulin-Transferrin-Selenium (ITS) (100X) and Insulin-Transferrin-Selenium (ITS) Select (100X) supplements?

ITS Select supplements are animal origin-free (AOF) versions of the ITS supplements offered by Thermo Fisher Scientific.

ITS Select supplements use recombinant versions of both proteins in the formulation: insulin and transferrin.

All other versions of ITS, those without the Select designation, utilize recombinant insulin and a human-derived holo transferrin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the individual concentrations of insulin, transferrin, and selenium in the Insulin-Transferrin-Selenium (ITS) (100X) supplements?

All ITS and ITS Select supplements have the following concentrations of their components:

Insulin: 1.0 g/L
Transferrin: 0.55 g/L
Selenium: 0.00067 g/L

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I store aliquots of Insulin-Transferrin-Selenium (ITS) (100X) supplements at -20 degrees C without compromising the integrity of the product?

We recommend storing all ITS and ITS Select supplements at 2-8 degrees C as there is a chance that the insulin will precipitate upon freezing and thawing.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Are all Insulin-Transferrin-Selenium (ITS) (100X) supplements offered by Thermo Fisher Scientific animal origin-free?

We offer both animal origin and animal origin-free versions of our ITS supplements. The animal origin-free product versions are referred to as ITS Select supplements.

Insulin-Transferrin-Selenium (ITS-G) (100X) (Cat. No. 41400045), Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) (100X) (Cat. No. 51500056), and Insulin-Transferrin-Selenium-Sodium Pyruvate (ITS-A) (100X) (Cat. No. 51300044) are not animal origin-free.

Animal origin-free product versions are available for the ITS-G and ITS-X formulations: Insulin-Transferrin-Selenium (ITS-G) Select Supplement (100X) (Cat. No. A4000046501 and A4000046502) and Insulin-Transferrin-Selenium-Ethanolamine (ITS-X) Select Supplement (100X) (Cat. No. A4000046401 and Cat. No. A4000046402.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all 胰岛素-转铁蛋白-硒 (ITS -G) (100X) FAQs

引用和文献 (4)

引用和文献
Abstract
A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.
Authors:Bonanini F,Singh M,Yang H,Kurek D,Harms AC,Mardinoglu A,Hankemeier T
Journal:Experimental cell research
PubMed ID:38499143
Protocol to generate human liver spheroids to study liver fibrosis induced by metabolic stress.
Authors:Kim HY,Lee W,Liu X,Jang H,Sakane S,Carvalho-Gontijo Weber R,Diggle K,Kerk SA,Metallo CM,Kisseleva T,Brenner DA
Journal:STAR protocols
PubMed ID:38833372
Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models. Here, we present a protocol to generate 3D human liver spheroids that contain all the liver cell types and mimic “livers in a dish.” We describe strategies to induce ... More
Collective cell migration requires suppression of actomyosin at cell-cell contacts mediated by DDR1 and the cell polarity regulators Par3 and Par6.
Authors:Hidalgo-Carcedo C, Hooper S, Chaudhry SI, Williamson P, Harrington K, Leitinger B, Sahai E,
Journal:Nat Cell Biol
PubMed ID:21170030
Collective cell migration occurs in a range of contexts: cancer cells frequently invade in cohorts while retaining cell-cell junctions. Here we show that collective invasion by cancer cells depends on decreasing actomyosin contractility at sites of cell-cell contact. When actomyosin is not downregulated at cell-cell contacts, migrating cells lose cohesion. ... More
Human cardiomyocyte progenitor cells differentiate into functional mature cardiomyocytes: an in vitro model for studying human cardiac physiology and pathophysiology.
Authors:Smits AM, van Vliet P, Metz CH, Korfage T, Sluijter JP, Doevendans PA, Goumans MJ,
Journal:Nat Protoc
PubMed ID:19197267
To date, there is no suitable in vitro model to study human adult cardiac cell biology. Although embryonic stem cells are able to differentiate into cardiomyocytes in vitro, the efficiency of this process is very low. Other methods to differentiate progenitor cells into beating cardiomyocytes rely on coculturing with rat ... More