TaqMan™ RNase P 检测试剂盒

Applied Biosystems™

TaqMan™ RNase P 检测试剂盒

Applied Biosystems™ TaqMan™ RNase P 检测试剂盒提供了使用 5’ 核酸酶测定来检测和量化人 RNase P了解更多信息

货号	数量
4316831	100 reactions

货号 4316831

价格（CNY)

3,533.00

添加至购物车

100 reactions

价格（CNY)

3,533.00

添加至购物车

Applied Biosystems™ TaqMan™ RNase P 检测试剂盒提供了使用 5’ 核酸酶测定来检测和量化人 RNase P 基因的基因组拷贝所需的组分。人 RNase P 基因是一种单拷贝基因，负责编码 RNase P 酶的 RNA 部分。这种 5’ 核酸酶测定采用 TaqMan™ 通用 PCR 预混液或 TaqMan™ PCR 核心试剂，使用提供的人基因组 DNA 作为模板进行聚合酶链反应 (PCR)。

•预先设计的引物和 TaqMan™ 探针消除了测定设计的需要
• 易于使用的引物和 TaqMan™ 探针混合物较大限度地缩短了测定构建时间
• 《快速测定开发指南》可较大限度缩短优化时间

包含综合指南
Applied Biosystems™ 制定了一整套指南，可确保使用 Applied Biosystems™ 实时荧光定量 PCR 试剂和仪器成功完成测定。这些指南简单易行。许多传统变量，例如氯化镁浓度和热循环方案本身已经标准化，极大地缩短了测定开发时间。

内置标准化
所有 Applied Biosystems™ 实时荧光定量 PCR 试剂盒均包含了被动内部参比，以对非 PCR 相关荧光波动进行标准化。使用被动内参进行标准化将尽可能地减少各种原因（例如移液误差和样品蒸发）导致的孔间差异。当多个探针（具有不同的报告基团染料）组合在单管中时，这种被动参比对于得到准确结果同样至关重要。

注：有关有限的标签许可和商标信息，请参见用户手册或包装说明书。仅供科研使用。不适用于诊断用途。

仅供科研使用。不可用于诊断程序。

校准类型被动内参标准品

控制模板人基因组 DNA

产品规格管装

内部探针修改TAMRA™ Quencher (3')，FAM (5')

反应次数100 次反应

产品线TaqMan

产品类型实时荧光定量 PCR 试剂

数量100 reactions

运输条件干冰

足够用于100 次反应

靶标RNase P

高 GC PCR 扩增效果低

标签或染料FAM

PCR 方法qPCR

反应速度标准

Unit SizeEach

内容与储存

对照引物探针套件&模板

常见问题解答 (FAQ)

With the Oncomine Focus Assay, Chef-Ready Library kit, where can I find the combined barcoded AmpliSeq libraries when the run is complete?

After the completion of a run, the Ion Chef Instrument holds the barcoded libraries in the tube loaded in Position D of the Reagents cartridge. To avoid fluid loss due to evaporation, remove and cap the tube of combined barcoded libraries as soon as possible after run completion. Do not leave the tube in the instrument longer than 24 hours after the start of the run. After 24 hours from the start of the run, the instrument chiller will stop actively cooling, and the sample will be held at 27 degrees C.

The tube in Position D of the Ion AmpliSeq Chef Reagents DL8 Cartridge will contain 700 µL of combined barcoded libraries at completion of the run. The libraries are at >>100 pM (total combined library concentration) and are ready to use in template preparation. One can store unused portions of combined libraries at 4 to 8 degrees C for up to 1 month. For longer-term storage, store at -30 to -10 degrees C.

With the Oncomine Focus Assay, Chef-Ready Library kit, can I process fewer than 8 samples per Ion AmpliSeq run on the Ion Chef System?

Yes, fewer than 8 samples may be processed per run but keep in mind that a run consumes kit reagents for 8 samples regardless of the sample number.

If I process less than 8 samples using the Oncomine Focus Assay, Chef-Ready Library kit, do I need to quantitate the final combined output library?

If you are processing ≤5 samples, we recommend that you quantify your output combined library by qPCR to ensure that an optimal concentration is used in templating reactions.

If you are processing fewer than 8 samples, it is preferable to add replicates or positive control samples to the run. Otherwise, pipet 15 µL of nuclease-free water as non-template control into column 1 wells that do not contain a DNA sample.

What is the RNA input for the Oncomine Focus Assay, Chef-Ready Library kit?

Each reverse transcription reaction requires 10 ng of DNase-treated total RNA (≥1.43 ng/µL).

What is the DNA input for the Oncomine Focus Assay, Chef-Ready Library kit?

For each target amplification reaction, use 300-30,000 copies of DNA (10 ng of mammalian gDNA) from normal or FFPE tissue.

View all TaqMan™ RNase P 检测试剂盒 FAQs