Primer Express™ 软件 v3.0.1 许可证
Primer Express™ 软件 v3.0.1 许可证
Applied Biosystems™

Primer Express™ 软件 v3.0.1 许可证

Primer Express 软件 v3.0.1 允许您自行设计引物和探针,并利用 TaqMan 及 SYBR Green I了解更多信息
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货号数量
43639911 user
43639935 users
货号 4363991
价格(CNY)
-
数量:
1 user
Primer Express 软件 v3.0.1 允许您自行设计引物和探针,并利用 TaqMan 及 SYBR Green I 染料化学试剂进行基因定量和等位基因区分 (SNP) 实时荧光定量 PCR 应用。Primer Express 软件专为配合我们的 StepOne、StepOnePlus、7300、7500、7500 Fast、7900HT、ViiA 7 和 QuantStudio 实时荧光定量 PCR 系统一起使用而开发,可提供定制的应用专用文件,用于绝对⁄相对定量测定和等位基因分型。

下载 Primer Express 软件 v3.0.1(需要许可密钥)>
查看详细介绍 v3.0.1 中增强功能的版本说明 >

• 能够为实时荧光定量 PCR 应用进行单个寡核苷酸设计。
•支持基于 TaqMan 和 SYBR Green I 染料化学试剂的测定。
•提供设计灵活性和易用性,并且几乎不需要优化。
•根据测定开发指南使用时,可提供稳健的测定性能。

易学的软件
Primer Express 软件 v3.0.1 非常灵活,易于学习和使用,并且几乎不需要优化。它包括专门针对使用基于 TaqMan 的试剂、推荐的引物⁄探针浓度和默认的热循环条件进行过优化的算法。Primer Express 软件还支持基于 SYBR Green I 染料的测定。这尽可能减少了测定优化的需要,并简化了设计过程。

如果已知探针和⁄或一个或多个引物,软件还允许进行定制设计。默认和可调参数(图 1)为所有用户提供了所需的灵活性

简单易用
Primer Express 软件 v3.0.1 具有简单和灵活的特点,与所有 StepOne、StepOnePlus、7300、7500、7500 Fast、7900HT、ViiA 7 和 QuantStudio 实时荧光定量 PCR 系统完全兼容。无论您的研究是否包括基因定量或等位基因区分,Primer Express 软件都适合您的实验室。

自动和手动引物⁄探针设计
Primer Express 软件 v3.0.1 会自动设计您的引物和探针,或者您可以进行手动设计。对于自动化设计,只需选择 TaqMan 或 TaqMan MGB 测定即可。如果您知道序列,只需要导入(或复制和粘贴)序列并快速查看结果。或者,如果您喜欢手动设计引物和探针,过程同样简单且直接。

与测定开发指南兼容
Primer Express 软件 v3.0.1 设计为与我们的实时荧光定量 PCR 仪器和试剂的标准使用指南相兼容。这些指南使得包括氯化镁浓度和热循环方案在内的传统变量应该可以使用试剂(酶、盐、缓冲液)的预混液以及定制的引物和探针设计实现标准化。通过允许每个微孔板同时运行多个测定,该方法大幅缩短了测定开发时间并提高了实验室效率,因为所有测定都需要相同的条件。
仅供科研使用。不可用于诊断程序。
规格
适用于(应用)实时 PCR (qPCR)
适用于(设备)7300 系统、7500 Fast 系统、7500 系统、QuantStudio™ 12k Flex、QuantStudio™ 3、QuantStudio™ 5、QuantStudio™ 6 Flex、QuantStudio™ 7、StepOne™、StepOnePlus™、ViiA™ 7 系统
操作系统Windows 10
产品线Primer Express™
数量1 user
软件类型引物设计
产品类型许可证
Unit SizeEach
内容与储存
Store at Room Temperature, Included w/ StepOne, StepOnePlus, 7300, 7500, 7500 Fast, 7900HT, ViiA 7, QuantStudio 6, QuantStudio 7, and QuantStudio 12k Flex, Runs computers on Windows 10 OS for 7500, 7500 Fast, QuantStudio 6, QuantStudio 7, and QuantStudio

常见问题解答 (FAQ)

I need to see all the primer and probe sequences from an assay design right away. How can I do this?

You can directly see all the primer and probe sequences from a test design right away using the Primer Express Software. Open the software and choose File => New. Choose either TaqMan MGB Quantification or TaqMan Quantification for Gene Expression Assay design. Copy/paste your gene sequence of interest into the new window and click on the green triangle to get primer/probe designs.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Can I use Primer Express software on Windows 10?

Yes, you can use Primer Express v3.0.1 on Win10 computers.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

When designing primer and probes, what is difference between green amplicons and red amplicons in the "Map" display of Primer Express 2.0 software?

Primer and probe sets that satisfy the optimal length and Tm requirements are in red. Sets that don't satisfy one or the other or both of these parameters are in blue or green.

I am trying to install Primer Express Software, version 2.0 on my new computer with Windows XP as the operating system. It is giving me an error message, "Could not open Primers Express Preferences, because there is not enough memory" when trying to install or open the software despite this being a new computer. What can I do?

The Primer Express 2.0 software was developed to work on Windows 2000 operating systems. The "not enough memory" error message is generated due to incompatibility with the newer processor in the computers and the algorithm of the Primer Express v2.0 software. We have developed a patch to help over come this error message. You can download this patch from our website by going to the software downloads option under the Technical Resources page on our website. Click on ABI PRISM 7000 Sequence Detection System, then click on the PC link next to Primer Express P4 Enabler. Also download the installation instructions by clicking on the Read Me link.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

I get the following message when trying to start the Primer Express software: "Error opening Archive file: Primer Express is unable to open the archive file. Please move this file to another directory and run the Primer Express software again to create a new Archive." How do I solve this problem?

This error message can occur while attempting to install the Primer Express software, or sometimes when launching the software after it has been installed on the computer. To create a new archive file, hold down Alt key (to the left of the space bar) while opening the Primer Express application. You should get the browser window shown below. Click on “New” to create a New Archive.

The name “PXArchive” will automatically populate in the file name field. You may keep that name or change it to something different. Once you've named the Archive file, click on save. You should now be able to continue with the installation or use of the Primer Express Software.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.