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引用和文献 (1122)
Applied Biosystems™
High-Capacity cDNA 反转录试剂盒
The High Capacity cDNA Reverse Transcription Kit (formerly the High Capacity cDNA Archive Kit) delivers extremely high-quality, single-stranded cDNA from了解更多信息
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| 货号 | 包括 | 反应次数 |
|---|---|---|
| 4368813 | Kit only | 1,000 次反应 |
| 4368814 | Kit only | 200 次反应 |
| 4374966 | Kit with Rnase Inhibitor | 200 次反应 |
| 4374967 | Kit with Rnase Inhibitor | 1,000 次反应 |
货号 4368813
价格（CNY)
9,127.00
飞享价
Ends: 31-Dec-2025
15,523.00共减 6,396.00 (41%)
Each
包括:
Kit only
反应次数:
1,000 次反应
价格（CNY)
9,127.00
飞享价
Ends: 31-Dec-2025
15,523.00共减 6,396.00 (41%)
Each
The High Capacity cDNA Reverse Transcription Kit (formerly the High Capacity cDNA Archive Kit) delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 μg input total RNA. Ideal for generating cDNA archives. The High Capacity cDNA Reverse Transcription Kit can convert up to 2 μg of input total RNA in a single 20 μl reaction. Reactions can be scaled up to 100 μl to generate 10 μg of cDNA from a single reaction The cDNA generated is ideal for a range of applications including real-time PCR, standard PCR, and microarrays.
• Dynamic Range – 10-fold dynamic range
• Sensitive – Generates cDNA from 0.02 to 2 μg input total RNA
• Flexibility – Tested with a variety of RNA templates including GC and AU-rich targets and RNAs expressed at low levels
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
• Dynamic Range – 10-fold dynamic range
• Sensitive – Generates cDNA from 0.02 to 2 μg input total RNA
• Flexibility – Tested with a variety of RNA templates including GC and AU-rich targets and RNAs expressed at low levels
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
仅供科研使用。不可用于诊断程序。
规格
最终产品类型第一链 cDNA
产品规格试剂盒
包括Kit only
反应次数1,000 次反应
数量1 Ea.
反应形式单独组分
试剂类型反转录
逆转录酶MultiScribe™
核糖核酸酶 H 活性是
运输条件干冰
尺寸（最终产品）长达 7 kb
原始材料RNA
技术反转录
最大浓度50 U/μL
适用于（应用）Real Time PCR (qPCR), RT-PCR
反应速度120 min。
Unit SizeEach
内容与储存
• 2 x 1 mL 10X RT 缓冲液
• 2 x 1 mL 10X RT 随机引物
• 1 x 1 mL 25X dNTP 混合物 (100 mM)
• 1 x 1 mL MultiScribe 逆转录酶 (50 U/μL)
在 -15°C 至 -25°C 下储存。
• 2 x 1 mL 10X RT 随机引物
• 1 x 1 mL 25X dNTP 混合物 (100 mM)
• 1 x 1 mL MultiScribe 逆转录酶 (50 U/μL)
在 -15°C 至 -25°C 下储存。
常见问题解答 (FAQ)
How long can I store the cDNA from my reverse transcription step?
Which kit should I use for the reverse transcription step for TaqMan Pri-miRNA assays (Cat. No. 4427012)?
Can I purchase the 10X RT Buffer from the High-Capacity cDNA Reverse Transcription Kit as a stand-alone item?
What is the concentration of the random primers in the High-Capacity cDNA Reverse Transcription Kit (Cat. no. 4368813)?
Is the 25X 100 mM dNTP Mix included in the High Capacity cDNA Reverse Transcription Kit (Cat. No. 4368813, 4368814) 100 mM each or 100 mM combined?
引用和文献 (1122)
引用和文献
Abstract
Hyaluronan-CD44-ERK1/2 regulate human aortic smooth muscle cell motility during aging.
Journal:The Journal of biological chemistry
PubMed ID:18077444
Increased hepatobiliary clearance of unconjugated thyroxine determines DMP 904-induced alterations in thyroid hormone homeostasis in rats.
Journal:Toxicological sciences : an official journal of the Society of Toxicology
PubMed ID:15673846
4-(3-pentylamino)-2,7-dimethyl-8-(2-methyl-4-methoxyphenyl)-pyrazolo-[1,5-a]-pyri midine (DMP 904) is a potent and selective antagonist of corticotropin releasing factor receptor-1 (CRF1 receptor) with an efficacious anxiolytic profile in preclinical animal models. In subchronic toxicity studies in Sprague-Dawley rats, DMP 904 produced thyroid follicular cell hypertrophy and hyperplasia, and a low incidence of follicular cell
Calcineurin/nuclear factor of activated T cells and MAPK signaling induce TNF-{alpha} gene expression in pancreatic islet endocrine cells.
Journal:J Biol Chem
PubMed ID:21059644
Cytokines contribute to pancreatic islet inflammation, leading to impaired glucose homeostasis and diabetic diseases. A plethora of data shows that proinflammatory cytokines are produced in pancreatic islets by infiltrating mononuclear immune cells. Here, we show that pancreatic islet alpha cells and beta cells express tumor necrosis factor-alpha (TNF-alpha) and other
TGF-beta regulates miR-206 and miR-29 to control myogenic differentiation through regulation of HDAC4.
Journal:J Biol Chem
PubMed ID:21324893
MicroRNAs (miRs) are emerging as prominent players in the regulation of many biological processes, including myogenic commitment and skeletal muscle formation. Members of the TGF-beta family can influence the proliferation and myogenic differentiation of cells, although it is presently not clear what role miRNAs play in the TGF-beta-mediated control of
Somatic cell plasticity and Niemann-Pick type C2 protein: fibroblast activation.
Journal:J Biol Chem
PubMed ID:21084287
A growing body of evidence points toward activated fibroblasts, also known as myofibroblasts, as one of the leading mediators in several major human pathologies including proliferative fibrotic disorders, invasive tumor growth, rheumatoid arthritis, and atherosclerosis. Niemann-Pick Type C2 (NPC2) protein has been recently identified as a product of the second