High-Capacity cDNA 反转录试剂盒
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High-Capacity cDNA 反转录试剂盒
Applied Biosystems™

High-Capacity cDNA 反转录试剂盒

The High Capacity cDNA Reverse Transcription Kit (formerly the High Capacity cDNA Archive Kit) delivers extremely high-quality, single-stranded cDNA from了解更多信息
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货号包括反应次数
4368814Kit only200 次反应
4374966Kit with Rnase Inhibitor200 次反应
4368813Kit only1,000 次反应
4374967Kit with Rnase Inhibitor1,000 次反应
货号 4368814
价格（CNY)
2,615.00
飞享价
Ends: 31-Dec-2025
4,448.00
共减 1,833.00 (41%)
Each
添加至购物车
包括:
Kit only
反应次数:
200 次反应
价格（CNY)
2,615.00
飞享价
Ends: 31-Dec-2025
4,448.00
共减 1,833.00 (41%)
Each
添加至购物车
The High Capacity cDNA Reverse Transcription Kit (formerly the High Capacity cDNA Archive Kit) delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 μg input total RNA. Ideal for generating cDNA archives. The High Capacity cDNA Reverse Transcription Kit can convert up to 2 μg of input total RNA in a single 20 μl reaction. Reactions can be scaled up to 100 μl to generate 10 μg of cDNA from a single reaction The cDNA generated is ideal for a range of applications including real-time PCR, standard PCR, and microarrays.
• Dynamic Range – 10-fold dynamic range
• Sensitive – Generates cDNA from 0.02 to 2 μg input total RNA
• Flexibility – Tested with a variety of RNA templates including GC and AU-rich targets and RNAs expressed at low levels

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
仅供科研使用。不可用于诊断程序。
规格
最终产品类型第一链 cDNA
产品规格试剂盒
包括Kit only
反应次数200 次反应
最佳反应温度37°C
数量1 Ea.
反应形式单独组分
试剂类型反转录
逆转录酶MultiScribe™
核糖核酸酶 H 活性是
运输条件干冰
尺寸（最终产品）长达 7 kb
原始材料RNA
技术反转录
最大浓度50 U/μL
适用于（应用）实时 PCR (qPCR)
反应速度120 min。
Unit SizeEach
内容与储存

• 10X RT 缓冲液,1 mL
• 10X RT 随机引物,1 mL
• 25X dNTP 混合物,0.2 mL (100 mM)
• MultiScribe 逆转录酶,2 x 0.1 mL (50 U/μL)

储存在 –25°C 至 –15°C 下。

常见问题解答 (FAQ)

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

Can one use the reagents from High Capacity RT kit to make cDNA for TaqMan MicroRNA Assay?

Yes. You can use the reagents from the High Capacity cDNA Kit PN 4368814 (without RNAse Inhibitor) or 4374966 (with RNAse Inhibitor). Make sure to use the reverse transcription primer provided from the TaqMan miRNA assay for the reverse transcription.

Which kit should I use for the reverse transcription step for TaqMan Pri-miRNA assays (Cat. No. 4427012)?

We recommend the following kits:

  • High-Capacity cDNA Reverse Transcription Kit (Cat. No. 4374966).
  • High-Capacity RNA-to-cDNA Kit (Cat. No. 4387406).


Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Can I purchase the 10X RT Buffer from the High-Capacity cDNA Reverse Transcription Kit as a stand-alone item?

Yes, the 10X RT Buffer can be purchased as a stand-alone item (Cat. No. N8080010).

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Is the 25X 100 mM dNTP Mix included in the High Capacity cDNA Reverse Transcription Kit (Cat. No. 4368813, 4368814) 100 mM each or 100 mM combined?

It contains a blend of 100 mM solution of dNTP (25 mM each of dATP, dCTP, dGTP and dTTP).