High-Capacity cDNA 反转录试剂盒

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引用和文献 (1122)

Applied Biosystems™

High-Capacity cDNA 反转录试剂盒

Name: High-Capacity cDNA 反转录试剂盒
SKU: 4368814
Price: 2615.00 CNY

The High Capacity cDNA Reverse Transcription Kit (formerly the High Capacity cDNA Archive Kit) delivers extremely high-quality, single-stranded cDNA from了解更多信息

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货号	包括	反应次数
4368814	Kit only	200 次反应
4374966	Kit with Rnase Inhibitor	200 次反应
4368813	Kit only	1,000 次反应
4374967	Kit with Rnase Inhibitor	1,000 次反应

4 选项

货号 4368814

价格（CNY)

2,615.00

飞享价

Ends: 31-Dec-2025

4,448.00

共减 1,833.00 (41%)

Each

添加至购物车

包括:

Kit only

反应次数:

200 次反应

价格（CNY)

2,615.00

飞享价

Ends: 31-Dec-2025

4,448.00

共减 1,833.00 (41%)

Each

添加至购物车

The High Capacity cDNA Reverse Transcription Kit (formerly the High Capacity cDNA Archive Kit) delivers extremely high-quality, single-stranded cDNA from 0.02 to 2 μg input total RNA. Ideal for generating cDNA archives. The High Capacity cDNA Reverse Transcription Kit can convert up to 2 μg of input total RNA in a single 20 μl reaction. Reactions can be scaled up to 100 μl to generate 10 μg of cDNA from a single reaction The cDNA generated is ideal for a range of applications including real-time PCR, standard PCR, and microarrays.
• Dynamic Range – 10-fold dynamic range
• Sensitive – Generates cDNA from 0.02 to 2 μg input total RNA
• Flexibility – Tested with a variety of RNA templates including GC and AU-rich targets and RNAs expressed at low levels

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

仅供科研使用。不可用于诊断程序。

规格

最终产品类型第一链 cDNA

产品规格试剂盒

包括Kit only

反应次数200 次反应

最佳反应温度37°C

数量1 Ea.

反应形式单独组分

试剂类型反转录

逆转录酶MultiScribe™

核糖核酸酶 H 活性是

运输条件干冰

尺寸（最终产品）长达 7 kb

原始材料RNA

技术反转录

最大浓度50 U/μL

适用于（应用）实时 PCR (qPCR)

反应速度120 min。

Unit SizeEach

内容与储存

• 10X RT 缓冲液,1 mL
• 10X RT 随机引物,1 mL
• 25X dNTP 混合物,0.2 mL (100 mM)
• MultiScribe 逆转录酶,2 x 0.1 mL (50 U/μL)

储存在 –25°C 至 –15°C 下。

常见问题解答 (FAQ)

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

Can one use the reagents from High Capacity RT kit to make cDNA for TaqMan MicroRNA Assay?

Yes. You can use the reagents from the High Capacity cDNA Kit PN 4368814 (without RNAse Inhibitor) or 4374966 (with RNAse Inhibitor). Make sure to use the reverse transcription primer provided from the TaqMan miRNA assay for the reverse transcription.

Which kit should I use for the reverse transcription step for TaqMan Pri-miRNA assays (Cat. No. 4427012)?

We recommend the following kits:

High-Capacity cDNA Reverse Transcription Kit (Cat. No. 4374966).
High-Capacity RNA-to-cDNA Kit (Cat. No. 4387406).

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Can I purchase the 10X RT Buffer from the High-Capacity cDNA Reverse Transcription Kit as a stand-alone item?

Yes, the 10X RT Buffer can be purchased as a stand-alone item (Cat. No. N8080010).

Find additional tips, troubleshooting help, and resources within our PCR and cDNA Synthesis Support Center.

Is the 25X 100 mM dNTP Mix included in the High Capacity cDNA Reverse Transcription Kit (Cat. No. 4368813, 4368814) 100 mM each or 100 mM combined?

It contains a blend of 100 mM solution of dNTP (25 mM each of dATP, dCTP, dGTP and dTTP).

引用和文献 (1122)

引用和文献

Abstract

Hyaluronan-CD44-ERK1/2 regulate human aortic smooth muscle cell motility during aging.

Authors:Vigetti D,Viola M,Karousou E,Rizzi M,Moretto P,Genasetti A,Clerici M,Hascall VC,De Luca G,Passi A

Journal:The Journal of biological chemistry

PubMed ID:18077444

Increased hepatobiliary clearance of unconjugated thyroxine determines DMP 904-induced alterations in thyroid hormone homeostasis in rats.

Authors:Wong H,Lehman-McKeeman LD,Grubb MF,Grossman SJ,Bhaskaran VM,Solon EG,Shen HS,Gerson RJ,Car BD,Zhao B,Gemzik B

Journal:Toxicological sciences : an official journal of the Society of Toxicology

PubMed ID:15673846

4-(3-pentylamino)-2,7-dimethyl-8-(2-methyl-4-methoxyphenyl)-pyrazolo-[1,5-a]-pyri midine (DMP 904) is a potent and selective antagonist of corticotropin releasing factor receptor-1 (CRF1 receptor) with an efficacious anxiolytic profile in preclinical animal models. In subchronic toxicity studies in Sprague-Dawley rats, DMP 904 produced thyroid follicular cell hypertrophy and hyperplasia, and a low incidence of follicular cell ... More

Calcineurin/nuclear factor of activated T cells and MAPK signaling induce TNF-{alpha} gene expression in pancreatic islet endocrine cells.

Authors:Lawrence MC, Naziruddin B, Levy MF, Jackson A, McGlynn K

Journal:J Biol Chem

PubMed ID:21059644

Cytokines contribute to pancreatic islet inflammation, leading to impaired glucose homeostasis and diabetic diseases. A plethora of data shows that proinflammatory cytokines are produced in pancreatic islets by infiltrating mononuclear immune cells. Here, we show that pancreatic islet alpha cells and beta cells express tumor necrosis factor-alpha (TNF-alpha) and other ... More

TGF-beta regulates miR-206 and miR-29 to control myogenic differentiation through regulation of HDAC4.

Authors:Winbanks CE, Wang B, Beyer C, Koh P, White L, Kantharidis P, Gregorevic P

Journal:J Biol Chem

PubMed ID:21324893

MicroRNAs (miRs) are emerging as prominent players in the regulation of many biological processes, including myogenic commitment and skeletal muscle formation. Members of the TGF-beta family can influence the proliferation and myogenic differentiation of cells, although it is presently not clear what role miRNAs play in the TGF-beta-mediated control of ... More

Disordered macrophage cytokine secretion underlies impaired acute inflammation and bacterial clearance in Crohn's disease.

Authors:Smith AM, Rahman FZ, Hayee B, Graham SJ, Marks DJ, Sewell GW, Palmer CD, Wilde J, Foxwell BM, Gloger IS, Sweeting T, Marsh M, Walker AP, Bloom SL, Segal AW

Journal:J Exp Med

PubMed ID:19652016

The cause of Crohn's disease (CD) remains poorly understood. Counterintuitively, these patients possess an impaired acute inflammatory response, which could result in delayed clearance of bacteria penetrating the lining of the bowel and predispose to granuloma formation and chronicity. We tested this hypothesis in human subjects by monitoring responses to ... More

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