TaqMan Fast Advanced 预混液在一系列 qPCR 仪器平台上以更短的时间提供准确的定量和可靠性。即使是具有挑战性的靶标,它也能在单次反应和双重反应中提供一流的性能,包括较大的可量化范围。TaqMan Fast Advanced了解更多信息
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货号
数量
4444556
1 x 1 mL
4444557
1 x 5 mL
4444963
2 x 5 mL
4444964
5 x 5 mL
4444965
10 x 5 mL
4444558
1 x 50 mL
6 选项
货号 4444556
价格(CNY)
1,470.00
Each
添加至购物车
数量:
1 x 1 mL
价格(CNY)
1,470.00
Each
添加至购物车
TaqMan Fast Advanced 预混液在一系列 qPCR 仪器平台上以更短的时间提供准确的定量和可靠性。即使是具有挑战性的靶标,它也能在单次反应和双重反应中提供一流的性能,包括较大的可量化范围。TaqMan Fast Advanced 预混液包含 AmpliTaq Fast DNA 聚合酶、尿嘧啶-N-糖基化酶 (UNG)、加入 dUTP 的 dNTP、ROX 染料(被动参比)和优化的缓冲液组分。它以 2X 浓度提供。
适用于(应用)Gene Expression, DNA Quantitation, Presence/Absence, miRNA analysis
形式液体
高 GC PCR 扩增效果高
PCR 方法qPCR
反应速度快速
Unit SizeEach
内容与储存
含有1管 (1 mL) 2X TaqMan Fast Advanced 预混液,足以进行100次反应
TaqMan Fast Advanced 预混液含有 AmpliTaq Fast DNA 聚合酶、尿嘧啶-N 糖基化酶 (UNG)、dNTP(含 dUTP)、ROX 染料(惰性参比染料)和经优化的缓冲液。
储存在 4°C 下。
常见问题解答 (FAQ)
What can I do to improve the sensitivity of my qPCR assay?
If you are targeting a low-abundance gene, you may have trouble getting Ct values in a good, reliable range (Ct > 32). To increase the sensitivity of the assay, you may want to consider the following:
- Increase the amount of RNA input into your reverse transcription reaction, if possible
- Increase the amount of cDNA in your qPCR reaction (20% by volume max)
- Try a different reverse transcription kit, such as our SuperScript VILO Master Mix, for the highest cDNA yield possible
- Consider trying a one-step or Cells-to-CT type workflow (depending on your sample type)
How do I set the baseline for my qPCR experiment?
Most times your instrument software can automatically set a proper baseline for your data. Check out our short video, Understanding Baselines, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=5BjFAJHW-bE).
How do I set the threshold for my qPCR experiment?
In most cases your instrument software can automatically set a proper threshold for your data. Check out our short video, Understanding Thresholds, for more information on how to set them (https://www.youtube.com/watch?feature=player_embedded&v=H_xsuRQIM9M).
I am not getting any amplification with my TaqMan Assay or SYBR Green primer set. What is causing this?
There could be several reasons for no amplification from an assay or primer set. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/no-amplification.html) for more details.
I am getting amplification in my no-template control (NTC) wells in my qPCR experiment. What is causing this?
There could be several reasons for amplification in a NTC well. Please see these examples and suggested solutions in our Real-Time Troubleshooting Tool (https://www.thermofisher.com/us/en/home/life-science/pcr/real-time-pcr/qpcr-education/real-time-pcr-troubleshooting-tool/gene-expression-quantitation-troubleshooting/amplification-no-template-control.html) for more details.