MagMAX™ Pathogen RNA/DNA Kit - FAQs

View additional product information for MagMAX™ Pathogen RNA/DNA Kit - FAQs (4462359)

13 product FAQs found

我可以编辑出厂自带的KingFisher Flex实验方案吗？

不能，出厂实验方案不能编辑，但是您可以使用“另存为”功能对其重命名，然后将它作为一个新的版本来编辑。

我可以在KingFisher Flex仪器上使用其它厂家生产的磁珠吗？

对于核酸提取，我们建议使用我们的MagMAX试剂盒和MagJET试剂盒。其它大小在0.5到10 µm间的磁珠应该也可以使用。但是，您需要自己用Thermo Scientific BindIt软件编写您自己的KingFisher实验方案，并自己验证/优化这一实验方案。

我想使用KingFisher Flex仪器从大体积的液体样本中提取核酸。我可以使用比实验方案中建议的体积更多的样本吗？

可以，可将样品分装。在进行下一步之前，设置程序使得磁头可以从多个孔内收集样本。确保不要过量加样。

使用KingFisher Flex仪器时我可以扩大我的反应体积吗？

可以，反应体积可以扩大，并且所有其它体积（例如洗涤体积）需要以相同倍数扩大。除非板的容积允许，我们强烈建议反应体积扩大不应超过2倍。

所有Applied Biosystems MagMAX试剂盒都能用于Thermo Scientific KingFisher Flex 磁珠处理仪吗？

是的，所有MagMAX试剂盒都能用于KingFisher Flex 磁珠处理仪。

What type of samples can I use with the MagMAX Pathogen RNA/DNA kit?

The MagMAX Pathogen RNA/DNA Kit is designed for rapid purification of nucleic acid (RNA and DNA) from the following sample types:

- Low-cell content samples including serum, plasma, swabs (nasal, tracheal, and cloacal), ear notches
- Whole blood
- Semen
- Oral fluid
- Feces
- Biomed Diagnostics InPouch TF (Tritrichomonas foetus) culture

Do you have a protocol for manual purification using a magnetic stand with the MagMAX Pathogen RNA/DNA Kit?

The manual isolation protocol for the MagMax Pathogen RNA/DNA Kit can be found in the MagMAX Pathogen RNA/DNA Kit Protocol , page 49.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Which beads should I use with the MagMax Pathogen RNA/DNA Kit (Cat. No. 4462359)?

We recommend using DNA Binding Beads for MagMAX-96 DNA Multi-Sample Kit (Cat. No. 4489112) with the MagMax Pathogen RNA/DNA Kit.

Can I edit the manufactured KingFisher Flex protocols?

No, manufactured protocols are locked for editing, but they can be renamed using the ‘save as' function and edited as a new version.

Can I use magnetic beads from other vendors with the KingFisher Flex instrument?

For nucleic acid extraction, we recommend using our MagMAX kits and MagJET kits. Other magnetic beads should work well as long as they are 0.5 to 10 µm in size. However, you will have to write your own KingFisher protocols using the Thermo Scientific BindIt software and validate/optimize the protocol on your own.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

I am working with liquid samples with high volume and want to extract nucleic acid from them using the KingFisher Flex instrument. Can I use more volume than the protocol advises?

Yes, this can be done by sample splitting. Set the protocol so that the magnet will collect samples from multiple wells before continuing to the next step. Be sure not to overload.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Can I scale up my reaction volume with the KingFisher Flex instrument?

Yes, reaction volumes can be scaled up, and all other volumes (i.e. wash volumes) will have to be increased by the same fold difference. It is generally not recommended to scale up reaction volumes more than 2-fold unless the plate's volume capacity will allow it.

Are all of the Applied Biosystems MagMAX kits validated for the KingFisher Flex Magnetic Particle Processor?

Yes, all MagMAX kits can be used with the KingFisher Flex Magnetic Particle Processor.