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View additional product information for ProFlex™ Sample Blocks - FAQs (4483637, 4484072, 4484071, 4484074, 4483638)
20 product FAQs found
应确认您使用的是最新版固件，因为新版固件解决了许多锁屏问题。可点击这里(https://www.thermofisher.com/cn/zh/home/technical-resources/software-downloads/proflex-pcr-system.html)下载最新版固件。如果您无法进入主菜单，可尝试关闭仪器，等待1分钟，然后在无任何附加模块的情况下重启仪器。
请遵循关于如何将ProFlex PCR系统连接到移动设备的指南。
1.使用无线或有线连接，将您的ProFlex PCR仪连接到网络。
注意：参见第2章的“如何创建ProFlex系统的无线连接”进行无线连接设置，或参考 ProFlex PCR系统用户指南来设置ProFlex PCR系统的有线连接。
2.记录仪器序列号和UUID号。
a.在主界面选择“设置”，打开“设置”界面。
b.在“设置”界面，选择“关于仪器”。
c.在“关于仪器”界面，选择“关于仪器”。
d.记录仪器序列号和UUID。
3.打开Life Technologies服务器连接。 a.在主界面选择“设置”，打开“设置”界面。
b.在“设置”界面，选择“设备设置”。
c.在“设备设置”界面，选择“远程服务”。
d.使用“开-关”切换按钮，开启“远程服务”。点击“OK”。
为设置移动设备的移动连接，请遵循以下步骤：
1.在您的手机中下载“PCR Essentials移动应用程序”。
2.在应用程序中，选择“我的ProFlex”选项。
3.输入您的thermofisher.com的用户名和密码。
注意： 如果您尚无thermofisher.com的用户名和密码，可以使用手机应用上的链接进行注册。
4.输入ProFlex仪器的序列号，可按您的意愿添加一个仪器别名。选择“添加设备”。
5.当您把仪器保存在您的账户中之后，下次登录时，该仪器将出现在“MYPROFLEXES”界面中。
6.您也可选择“添加设备”选项并重复步骤4和步骤5，在您的列表中添加更多仪器。
首先，应确认您使用的是最新版固件。新版固件更正了许多无线和移动应用程序问题。点击这里(https://www.thermofisher.com/cn/zh/home/technical-resources/software-downloads/proflex-pcr-system.html)可查看固件更新。
为创建ProFlex PCR仪的无线连接，请遵循下述步骤：
1.打开ProFlex仪器电源，在主界面选择“设置”。
2.在“设置”界面选择“设备设置”。
3.在“设备设置”界面选择“网络连接”。在“网络连接类型”界面选择“无线连接”。
将出现“搜索无线网络”界面，无线符号将变为激活状态。选择“取消”退出界面。
注意：初始设置时，如果在“网络连接类型”界面选择“有线连接”选项，当您选择了“静态IP”有线连接选项，您将需要输入您的IP地址；当您选择了“动态IP”有线连接选项，则IP地址会自动填充。
4.检测到无线连接后，在“选择网络”界面下将出现可用网络列表。点击您选择的网络名称或点击“加入其他网络”。
5.在“查找和加入网络”界面，输入网络的名称和安全类型。
注意： 当您选中“网络名称”输入框时，会出现键盘协助您填写。
6.在“安全类型”下拉菜单中，点击选择安全类型并在界面中输入相关信息
注意： 请联系您的IT系统管理员，获取安全类型信息。
◦WEP
◦WPA个人
◦WPA2个人
◦WPA企业
◦WPA2企业
7.选择“加入”或“取消”，以继续或退出“查找和加入网络”界面。
8.根据您选择的安全类型，输入正确的密码并选择“加入”。
若您输入了正确信息，将出现“网络连接完成”界面，可点击“OK”继续。若您输入了错误信息，将出现“网络连接失败”界面，您将需要回到安全类型界面解决问题。点击“OK”继续。
如果使用了错误的塑料耗材，或者没有使用正确的托盘/固定器，可能会发生这种情况。请查看说明书中关于正确上样的指南部分。此外，您还可以从这里(https://www.thermofisher .com/us/en/home/lifescience/pcr/pcr-plastics.html)查看兼容的塑料耗材：
Veriti热循环仪 - 上样(手册第3-5页)
ProFlex PCR系统 -上样(手册第25页)
2720热循环仪 - 上样(手册第3-5页)
如需设定一个恒定温度，请在程序中选择无限（∞) 标识。
在ProFlex PCR热循环仪中，96孔与3x32孔模块均装备了创新的VeriFlex模块用于提升PCR功能。六个分离的Peltier模块（在3x32孔模块中，-每1个模块（32孔）具有2个Peltier模块）提供最大的配置多样性和灵活性，同时提供两个关键优势：
1.PCR条件优化：每一模块均可单独设置一个特定温度，非常适合用户在PCR条件优化过程中对温度进行精确控制。
2.运行更多实验条件：六个Peltier模块也适用于在同一反应中设定六个不同的退火温度。
Auto Delta特性能够帮助您在循环阶段中逐渐增高或降低每一循环某一步骤的温度。其中一个常见应用就是降落PCR（touchdown PCR）。
96孔和3x32孔系统拥有6.0°C/秒的最大模块变温速率，双96孔和双平板模块的最大模块变温速率为2.0°C/秒。
孔板与塑料耗材均为特定模块类型所专用。有关您的模块类型兼容的塑料耗材列表，请参阅 此表。
ProFlex PCR仪可采用四种不同的模块，只需轻按开关即可进行更换。这其中也包括首创的3x32孔独立模块。这一模块能够同时运行三个完全独立、互不干扰的实验。双96孔模块用于匹配您的高通量测序需求，双平板模块支持OpenArray平板技术，用于QuantStudio 12K Flex实时荧光定量PCR系统的基因分型分析。
Make sure you have the most up-to-date firmware version, as this has fixed many screen-freezing issues. The latest firmware can be downloaded from here (https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/proflex-pcr-system.html). If you cannot get access to the main menu, try turning off the machine, letting it sit for 1 minute, and restarting it without any block attached.
Please follow these directions on how to set up Cloud and mobile connectivity in the ProFlex PCR System:
1. Ensure that the instrument is Cloud-enabled
Any ProFlex PCR System can be connected to Thermo Fisher Connect as long as it is using firmware version 1.2 or higher. You can check your current firmware version by:
a) Going to the Settings menu.
b) Selecting “About Instrument”.
2. Create a Thermo Fisher Connect account
a) From your Internet browser, go to thermofisher.com/connect
b) If you already have an account with Thermo Fisher Scientific, log in using the same name and password.
c) If you do not already have an account, follow the prompts to create one.
d) Once you have logged in to thermofisher.com/connect, an email will automatically be sent to you to verify your email address.
e) Open your email and click on the link to visit the Connect page to verify your account.
f) If you have done this successfully, you will receive a confirmation email telling you that you have connected to Thermo Fisher Connect.
3. Connect your ProFlex PCR machine to the internet using a wireless or wired connection.
-Note: Please refer to Chapter 2 of the ProFlex PCR System User Guide (https://tools.thermofisher.com/content/sfs/manuals/MAN0007697.pdf) for instructions on setting up of a wireless or wired connection.
4. Connect the instrument to Thermo Fisher Cloud
a) Touch Sign In.
b) Select Administrator when asked to choose screen name.
c) Log in. (The default password for the Administrator account is “password”.)
d) You will then be redirected back to the home screen. Go to Settings.
e) Select Remote Service.
f) Turn “on” Remote Service.
g) Touch My Profile on the home screen.
h) Touch the Cloud tab and enter your Thermo Fisher Connect account login and password.
i) Select Link Account. Upon successful authentication, the Administrator is linked to Thermo Fisher Connect.
-Subsequently, any user can log in and link the instrument to their Thermo Fisher Connect account by following steps 3-9 above.
-Note: The administrator account must be activated first to connect the instrument to the Cloud. Afterward, multiple users can create unique user profiles using their own login information. Each unique profile must have a unique Thermo Fisher Connect account.
5. Set mobile connectivity in a mobile device
a) Download and install the “Instrument Connect Mobile Application” on your mobile device.
-For an iPad™ or iPhone™ mobile device, download the application from the App Store™ by searching for ““Instrument Connect”.
-For Android™ devices, download the application from Google™ Play by searching for “Instrument Connect”.
b) Log in to the Instrument Connect mobile application using your Thermo Fisher Connect login and password.
-Once logged in, any Cloud-enabled instrument that has been linked to that account can be monitored.
Further information can be found here: https://assets.thermofisher.com/TFS-Assets/LSG/manuals/remote_connectivity_with_InstrumentConnect_PI.pdf
First, make sure you are running the most up to date firmware. Newer firmware versions have fixed many wireless and mobile app issues. Firmware updates can be found here: https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/proflex-pcr-system.html.
To set up the wireless connection in the ProFlex PCR System, please follow the steps outlined below:
1.Power on the ProFlex Instrument, and in the Home screen, touch Settings.
2.In the Settings screen, touch Device Settings.
3.In the Device Settings screen, touch Network Connection. In the Network Connection Type screen, touch Wireless.
The Search Wireless Network screen will come up and the wireless symbol will appear active. Touch Cancel to exit the screen.
Note: During initial setup, if you selected the Wired option in the Network Connection Type screen, you will be required to enter your IP address if you selected the Static IP wired option. If you selected the Dynamic IP wired option, the IP address is automatically populated.
4.Once a wireless connection has been detected, a list of the available networks will be displayed in the Choose Network screen. Touch the network name of your choice or touch Join Other Network.
5.In the Find and Join a Network screen, enter the name and security type of the network.
Note: When you touch in the Network Name field, a keypad will come up to facilitate making the entry.
6.From the Security type drop-down menu, touch to select the security type and enter the relevant information in the screen.
Note: Contact your IT Systems Administrator for information on security type.
You can select from the following options:
-WEP
-WPA Personal
-WPA2 Personal
-WPA Enterprise
-WPA2 Enterprise
7.Touch Join to continue or Cancel to exit from the Find and Join a Network screen.
8.Depending on the security type you have selected, enter the appropriate passwords and touch Join.
If you entered the correct information, the Network Connection Complete screen will appear. Touch OK to continue. If you entered incorrect information, the Network Connection Failed screen will come up and you will be required to go back to security type screen to resolve the issue. Touch OK to continue.
This can happen if the wrong plastics are used, or if the tubes are not being used with the correct tray/retainer set. Check the manual for directions on appropriate loading of the sample. Also, you can check for compatible plastics from here (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics.html).
Veriti Thermal Cycler - Loading Samples (p. 3-5 of the manual)
ProFlex PCR System - Loading Samples (p. 25 of the manual)
2720 Thermal Cycler - Loading Samples (p. 3-5 of the manual)
To add an infinite hold, select the infinity symbol for program.
In the ProFlex PCR System, the 96-well and 3 x 32-well blocks feature innovative VeriFlex Blocks for enhanced PCR functionality. Six separate Peltier blocks (2 on each 32-well block for 3 x 32-well) provide maximal versatility and flexibility and offer two key benefits:
1.PCR optimization: each block can be set with a specific temperature, which is ideal for precise control over PCR optimization.
2.Run more experiments: the six Peltier blocks can also be used to run up to six different annealing temperatures in the same run.
The AutoDelta feature allows you to incrementally increase or decrease the temperature or hold time with each cycle for a given step within a cycling stage. One common application for this is touchdown PCR.
The 96-well and 3 x 32-well system have a max block ramp rate of 6.0 degrees C/sec and the dual 96-well and dual flat blocks have a max block ramp rate of 2.0 degrees C/sec.
The plates and tubes will be specific to the block type. Please refer to our PCR Tubes, Plates & Accessories Plastics Selection Guide (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics.html) for a list of compatible plastics, based on your block type.
The ProFlex PCR System has 4 different blocks that can be changed with the flip of a switch, including the first-of-its-kind 3 x 32-well independent block. This block allows up to 3 experiments to be run simultaneously, completely independently of each other and without interference. A dual 96-well block is available for your high throughput sequencing needs and a dual flat block is available to support OpenArray plate technology for genotyping analysis on the QuantStudio 12K Flex Real-Time PCR System.