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Invitrogen
CD107b (LAMP-2) Monoclonal Antibody (eBioH4B4 (H4B4)), eFluor™ 660, eBioscience™
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图: 1 / 3
CD107b (LAMP-2) Antibody (50-1078-42) in Flow
Intracellular staining of Jurkat cells with Mouse IgG1 K Isotype Control eFluor® 660 (Product # 50-4714-82) (blue histogram) or Anti-Human CD107b (LAMP-2) eFluor® 660 (purple histogram). Total viable cells, as determined by Fixable Viability Dye eFluor® 450, were used for analysis.
产品信息
50-1078-42
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
eBioH4B4 (H4B4)
偶联物
eFluor™ 660
eFluor™ 660
eFluor™ 660
激发/发射光谱
651/668 nm
查看光谱
形式
Liquid
浓度
5 µL/Test
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2, with BSA
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The eBioH4B4 monoclonal antibody reacts with human CD107b, also known as lysosomal-associated membrane protein-2 (LAMP-2). CD107b is a highly glycosylated, type I transmembrane protein of approximately 105 kDa. It is expressed intracellularly in lysosomal/endosomal membranes in nearly all cells. It is also expressed on the surface of degranulating T cells (to a lesser extent than CD107a) and activated platelets as well as some cancer cells. In humans, mutations in CD107b results in a lysosomal glycogen storage disorder, known as Danon disease.
Applications Reported: This eBioH4B4 (H4B4) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This eBioH4B4 (H4B4) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of Jurkat cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
LAMP2 (Lysosome-associated membrane glycoprotein 2, CD107b) is a member of a family of membrane glycoproteins. LAMP2 provides selectins with carbohydrate ligands, and may play a role in tumor cell metastasis, and have a function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is approximately 46 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of about 100-130 kDa. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. LAMP2 is used to monitor a very selective form of autophagy called chaperone-mediated autophagy. LAMP 2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail. The LAMP protein has 3 variant forms: LAMP-2A, -2B, and 2C. LAMP2, together with CD107a/LAMP-1, is a major constituent of lysosomal membrane. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes, and LAMP2 is an important regulator in successful phagosomal maturation. LAMP2 deficiency causes an accumulation of autophagosomes in many tissues leading to cardiomyopathy and myopathy (Danon's disease).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: CD107 antigen-like family member B; CD107b; Igp96; LAMP-2; LAMP-2 Isoform 2A; LAMP-2 Variant A; LAMP-2C; LGP-96; Lysosome-associated membrane glycoprotein 2; RP23-193O17.2; unnamed protein product
基因别名: CD107b; DND; LAMP-2; LAMP2; LAMPB; LGP-96; LGP110
UniProt ID: (Human) P13473
Entrez Gene ID: (Human) 3920
protein targeting
autophagy
lysosomal lumen acidification
cellular response to starvation
protein import
protein catabolic process
negative regulation of protein complex assembly
regulation of protein stability
lysosomal lumen pH elevation
muscle cell cellular homeostasis
protein stabilization
chaperone-mediated autophagy
protein targeting to lysosome involved in chaperone-mediated autophagy
autophagosome maturation
negative regulation of NLRP3 inflammasome complex assembly
hydrogen ion transmembrane transport
lysosomal protein catabolic process
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