胰岛素-转铁蛋白-硒-氨基乙醇 (ITS -X) (100X)
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胰岛素-转铁蛋白-硒-氨基乙醇 (ITS -X) (100X)
胰岛素-转铁蛋白-硒-氨基乙醇 (ITS -X) (100X)
胰岛素-转铁蛋白-硒-氨基乙醇 (ITS -X) (100X)
Gibco™

胰岛素-转铁蛋白-硒-氨基乙醇 (ITS -X) (100X)

使用 Gibco™ 胰岛素-转铁蛋白-硒-乙醇胺 (ITS-X) 作为基础培养基补充剂,以减少培养细胞所用的胎牛血清 (FBS) 的量。ITS-X 溶液通常与 2–4%了解更多信息
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货号数量
5150005610 mL
货号 51500056
价格(CNY)
1,463.00
Each
添加至购物车
数量:
10 mL
价格(CNY)
1,463.00
Each
添加至购物车
使用 Gibco™ 胰岛素-转铁蛋白-硒-乙醇胺 (ITS-X) 作为基础培养基补充剂,以减少培养细胞所用的胎牛血清 (FBS) 的量。ITS-X 溶液通常与 2–4% FBS 联合使用,以支持需要乙醇胺的细胞类型,如支气管上皮细胞。

胰岛素可促进葡萄糖和氨基酸的摄取、脂肪形成、细胞内运输以及蛋白和核酸的合成。转铁蛋白是一种铁载体,也有助于降低氧自由基和过氧化物的毒性水平。硒(以亚硒酸钠提供)是谷胱甘肽过氧化物酶和其他蛋白的一种辅因子,在培养基中用作抗氧化剂。乙醇胺是磷酸甘油酯的前体,对质膜和细胞器的结构至关重要。

可提供完整配方

cGMP 生产和质量体系
Gibco™ ITS-X 在位于纽约格兰德岛的符合 cGMP 要求的工厂内生产。该工厂是在FDA登记的医疗器械生产商,且通过ISO 13485标准认证。
仅供科研使用。不可用于诊断程序。
规格
细胞类型哺乳动物细胞
最大浓度100 X
数量10 mL
有效期18 个月
运输条件室温
形式液体
产品类型转铁蛋白
无菌无菌过滤
加有添加剂Insulin
Unit SizeEach
内容与储存
储存条件:2°C 至 8°C
运输条件:室温
有效期:自生产之日起 18 个月

常见问题解答 (FAQ)

What is the buffer composition of the various Insulin-Transferrin-Selenium (ITS) (100X) and Insulin-Transferrin-Selenium (ITS) Select (100X) supplements?

All ITS and ITS Select supplements are formulated in Earle's Balanced Salt Solution (EBSS).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Insulin-Transferrin-Selenium (ITS) (100X) and Insulin-Transferrin-Selenium (ITS) Select (100X) supplements?

ITS Select supplements are animal origin-free (AOF) versions of the ITS supplements offered by Thermo Fisher Scientific.

ITS Select supplements use recombinant versions of both proteins in the formulation: insulin and transferrin.

All other versions of ITS, those without the Select designation, utilize recombinant insulin and a human-derived holo transferrin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the individual concentrations of insulin, transferrin, and selenium in the Insulin-Transferrin-Selenium (ITS) (100X) supplements?

All ITS and ITS Select supplements have the following concentrations of their components:

Insulin: 1.0 g/L
Transferrin: 0.55 g/L
Selenium: 0.00067 g/L

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I store aliquots of Insulin-Transferrin-Selenium (ITS) (100X) supplements at -20 degrees C without compromising the integrity of the product?

We recommend storing all ITS and ITS Select supplements at 2-8 degrees C as there is a chance that the insulin will precipitate upon freezing and thawing.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the source of transferrin in Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X) (Cat. No. 51500056)?

We use human-derived holo transferrin. This information can also be accessed in the Certificate of Origin (COO).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.