Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)
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Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)
Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)
Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)
Gibco™

Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X)

Gibco™ Insulin,-Transferrin-Selenium-Ethanolamine (ITS-X) is used as a basal medium supplement in order to reduce the amount of fetal bovine serumRead more
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Catalog NumberQuantity
5150005610 mL
Catalog number 51500056
Price (CNY)
1,463.00
Each
Add to cart
Quantity:
10 mL
Price (CNY)
1,463.00
Each
Add to cart

Gibco™ Insulin,-Transferrin-Selenium-Ethanolamine (ITS-X) is used as a basal medium supplement in order to reduce the amount of fetal bovine serum (FBS) used to culture cells. ITS-X solution is routinely used, in combination with 2 to 4% FBS, to support cell types that require ethanolamine, such as bronchial epithelial cells.

Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and may also help to reduce toxic levels of oxygen radicals and peroxide. Selenium, as sodium selenite is a co-factor for glutathione peroxidase and other proteins that is used as an anti-oxidant in media. Ethanolamine is a precursor of phospho-glycerides which are essential to the structure of the plasma membrane and cellular organelles.

The complete formulation is available.

cGMP Manufacturing and Quality System

Gibco™ ITS-X is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells
Concentration100 X
Quantity10 mL
Shelf Life18 Months
Shipping ConditionRoom Temperature
FormLiquid
Product TypeTransferrin
SterilitySterile-filtered
With AdditivesInsulin
Unit SizeEach
Contents & Storage
Storage conditions: 2°C to 8°C
Shipping conditions: Room temperature
Shelf life: 18 months from date of manufacture

Frequently asked questions (FAQs)

What is the buffer composition of the various Insulin-Transferrin-Selenium (ITS) (100X) and Insulin-Transferrin-Selenium (ITS) Select (100X) supplements?

All ITS and ITS Select supplements are formulated in Earle's Balanced Salt Solution (EBSS).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Insulin-Transferrin-Selenium (ITS) (100X) and Insulin-Transferrin-Selenium (ITS) Select (100X) supplements?

ITS Select supplements are animal origin-free (AOF) versions of the ITS supplements offered by Thermo Fisher Scientific.

ITS Select supplements use recombinant versions of both proteins in the formulation: insulin and transferrin.

All other versions of ITS, those without the Select designation, utilize recombinant insulin and a human-derived holo transferrin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the individual concentrations of insulin, transferrin, and selenium in the Insulin-Transferrin-Selenium (ITS) (100X) supplements?

All ITS and ITS Select supplements have the following concentrations of their components:

Insulin: 1.0 g/L
Transferrin: 0.55 g/L
Selenium: 0.00067 g/L

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I store aliquots of Insulin-Transferrin-Selenium (ITS) (100X) supplements at -20 degrees C without compromising the integrity of the product?

We recommend storing all ITS and ITS Select supplements at 2-8 degrees C as there is a chance that the insulin will precipitate upon freezing and thawing.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the source of transferrin in Insulin-Transferrin-Selenium-Ethanolamine (ITS -X) (100X) (Cat. No. 51500056)?

We use human-derived holo transferrin. This information can also be accessed in the Certificate of Origin (COO).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.