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          • Primary Antibodies ›
          • beta Amyloid Antibodies

          Invitrogen

          beta Amyloid Recombinant Rabbit Monoclonal Antibody (H31L21)

          43 篇参考文献
          查看其他41个beta Amyloid抗体

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          Cite beta Amyloid Recombinant Rabbit Monoclonal Antibody (H31L21)

          Additional Information:
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          • 抗体检测数据 (1)
          beta Amyloid Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          beta Amyloid Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          图: 1 / 1

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          beta Amyloid Antibody (700254) in IHC (P)

          Immunohistochemistry analysis of beta-Amyloid in formalin-fixed, paraffin-embedded human brain (left) and transgenic mouse tissue that expresses FAD mutant APP and PS1 (right) using a beta-Amyloid monoclonal antibody (Product # 700254) at a dilution of 1 µg/mL. Results show strong cytoplasmic staining in amyloid plaque. {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
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          beta Amyloid Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          beta Amyloid Recombinant Rabbit Monoclonal Antibody (H31L21)

          产品信息

          700254

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          -
          查看2篇已发表文章 2篇已发表文章

          免疫组化 (IHC)

          -
          查看18篇已发表文章 18篇已发表文章

          免疫组化(石蜡) (IHC (P))

          1 µg/mL
          查看2篇已发表文章 2篇已发表文章

          免疫组化(冰冻) (IHC (F))

          -
          查看3篇已发表文章 3篇已发表文章

          免疫组织化学(悬浮细胞) (IHC (Free))

          -
          查看1篇已发表文章 1篇已发表文章

          免疫细胞化学 (ICC/IF)

          -
          查看7篇已发表文章 7篇已发表文章

          流式细胞分析 (Flow)

          -
          查看1篇已发表文章 1篇已发表文章

          酶联免疫吸附实验 (ELISA)

          0.1-0.2 µg/mL
          查看4篇已发表文章 4篇已发表文章

          Luminex (Luminex)

          -
          查看1篇已发表文章 1篇已发表文章

          点杂交 (DB)

          -
          查看4篇已发表文章 4篇已发表文章
          产品规格

          种属反应

          Human, Mouse

          已发表种属

          Goat, Human, Mouse, Rat

          宿主/亚型

          Rabbit / IgG

          Expression System

          Expi293

          分类

          Recombinant Monoclonal

          类型

          Antibody

          克隆号

          H31L21

          抗原

          A peptide corresponding to amino acids 707-713 of P05067.
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          0.5 mg/mL

          纯化类型

          Protein A

          保存液

          PBS

          内含物

          0.09% sodium azide

          保存条件

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          运输条件

          Wet ice

          RRID

          AB_2532306

          产品详细信息

          Cross reactivity to Abeta [1-40] is not observed in sandwich ELISA. In addition, in antigen ELISA cross-reactivity is not observed with Abeta [1-37], Abeta [1-38], Abeta [1-40], or Abeta [1-43] when used at low antibody concentrations (up to 30 ng/mL).

          Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

          靶标信息

          Amyloid beta peptide (Abeta/Beta-amyloid) is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer's disease. Abeta peptide is 40-43 amino acids long and generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease, beta-secretase (BACE) which is synthesized as a propeptide and must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP, and a membrane-bound remnant. The remnant protein is processed by another protease, gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. The transmembrane glycoprotein, nicastrin, is associated with presinilins and has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide. Abeta is an extracellular filamentous protein component of amyloid cores, neuritic plaques and is also found as a deposit in neurofibrillary tangles. Alzheimer's disease, the most common cause of senile dementia, is characterized by abnormal filamentous protein deposits in the brain. Beta amyloid deposits are also detected in Lewy body dementia, Down's syndrome, amyloidosis (Dutch type), cerebroarterial amyloidosis (cerebral amyloid angiopathy) and in the Guam Parkinson-Dementia complex.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: ABPP; AG; Alzheimer disease; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; Amyloid; Amyloid b; Amyloid beta; amyloid beta (A4) precursor protein; amyloid beta A4 protein; amyloid beta precursor protein (ABPP); amyloid beta protein; amyloid beta-protein precursor; Amyloid precursor protein; amyloid protein; Amyloid β; Amyloid-beta (A4) precursor protein; Amyloid-beta A4 protein; Amyloid-beta precursor protein; Amyloidogenic glycoprotein; APP; APPI; appican; beta-amyloid A4; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); beta-amyloid precursor protein; Cerebral vascular amyloid peptide; CTF gamma; CTF-alpha; CVAP; OTTHUMP00000096096; Pan-Abeta; peptidase nexin-II; PN-II; PreA4; PreA4 751; protease nexin II; Protease nexin-II; testicular tissue protein Li 2; unnamed protein product

          查看更多 收起

          基因别名: A4; AAA; ABETA; ABPP; AD1; Adap; Ag; alpha-sAPP; APP; APPI; betaApp; CTFgamma; CVAP; E030013M08Rik; PN-II; PN2; preA4

          查看更多 收起

          UniProt ID: (Human) B2R5V1, (Mouse) P12023

          查看更多 收起

          Entrez Gene ID: (Human) 351, (Mouse) 11820

          查看更多 收起

          功能
          G-protein coupled receptor binding DNA binding chromatin binding serine-type endopeptidase inhibitor activity receptor binding frizzled binding insulin receptor binding integrin binding protein binding heparin binding peptidase activator activity enzyme binding peptidase inhibitor activity receptor activator activity acetylcholine receptor activator activity acetylcholine receptor binding apolipoprotein binding chemoattractant activity identical protein binding protein homodimerization activity heparan sulfate proteoglycan binding metal ion binding ephrin receptor binding transition metal ion binding protein heterodimerization activity receptor agonist activity low-density lipoprotein particle receptor binding RAGE receptor binding chaperone binding PTB domain binding growth factor receptor binding amylin binding protein serine/threonine kinase binding molecular function activator activity heparan sulfate binding protease inhibitor
          参与通路
          negative regulation of transcription from RNA polymerase II promoter microglial cell activation response to yeast astrocyte activation involved in immune response regulation of transcription from RNA polymerase II promoter regulation of translation cellular copper ion homeostasis endocytosis apoptotic process cell adhesion G-protein coupled receptor signaling pathway adenylate cyclase-activating G-protein coupled receptor signaling pathway adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Notch signaling pathway chemical synaptic transmission axonogenesis central nervous system development learning or memory learning memory mating behavior locomotory behavior axo-dendritic transport negative regulation of cell proliferation associative learning adult locomotory behavior visual learning response to lead ion regulation of gene expression positive regulation of macromolecule metabolic process positive regulation of gene expression negative regulation of gene expression response to auditory stimulus microglia development axon midline choice point recognition neuron remodeling dendrite development calcium-mediated signaling antibacterial humoral response antifungal humoral response regulation of Wnt signaling pathway extracellular matrix organization neuron projection development phospholipase D-activating G-protein coupled receptor signaling pathway positive regulation of chemokine production positive regulation of interferon-gamma production positive regulation of interleukin-1 beta production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production positive regulation of superoxide anion generation regulation of toll-like receptor signaling pathway ionotropic glutamate receptor signaling pathway regulation of multicellular organism growth lipoprotein metabolic process positive regulation of apoptotic process positive regulation of MAPK cascade positive regulation of neuron apoptotic process innate immune response positive regulation of nitric oxide biosynthetic process positive regulation of neuron differentiation positive regulation of G-protein coupled receptor protein signaling pathway positive regulation of Toll signaling pathway positive regulation of glycolytic process positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade astrocyte activation regulation of long-term neuronal synaptic plasticity collateral sprouting in absence of injury positive regulation of inflammatory response regulation of synapse structure or activity synapse organization defense response to Gram-negative bacterium defense response to Gram-positive bacterium positive regulation of calcium-mediated signaling positive regulation of cell activation cognition positive chemotaxis positive regulation of membrane protein ectodomain proteolysis regulation of protein metabolic process positive regulation of protein metabolic process protein homooligomerization protein tetramerization regulation of neurotransmitter uptake positive regulation of protein kinase B signaling low-density lipoprotein particle mediated signaling antimicrobial humoral immune response mediated by antimicrobial peptide protein trimerization positive regulation of ERK1 and ERK2 cascade response to interleukin-1 cellular response to copper ion cellular response to manganese ion cellular response to cAMP cellular response to norepinephrine stimulus positive regulation of monocyte chemotaxis negative regulation of canonical Wnt signaling pathway modulation of excitatory postsynaptic potential NMDA selective glutamate receptor signaling pathway positive regulation of gene expression, epigenetic positive regulation of cAMP/PKA signal transduction regulation of spontaneous synaptic transmission negative regulation of protein localization to nucleus regulation of beta-amyloid clearance negative regulation of long-term synaptic potentiation positive regulation of long-term synaptic potentiation positive regulation of NIK/NF-kappaB signaling regulation of calcium ion transmembrane transport via high voltage-gated calcium channel negative regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of dendritic spine maintenance positive regulation of amyloid precursor protein catabolic process regulation of endoplasmic reticulum stress-induced neuron intrinsic apoptotic signaling pathway negative regulation of blood circulation positive regulation of G protein-coupled receptor internalization positive regulation of endothelin production positive regulation of protein import cellular response to beta-amyloid regulation of presynapse assembly positive regulation of response to endoplasmic reticulum stress regulation of amyloid fibril formation positive regulation of amyloid fibril formation regulation of response to calcium ion amyloid fibril formation cellular response to nerve growth factor stimulus neuron projection maintenance positive regulation of T cell migration positive regulation of excitatory postsynaptic potential positive regulation of extrinsic apoptotic signaling pathway suckling behavior mRNA polyadenylation protein phosphorylation response to oxidative stress regulation of epidermal growth factor-activated receptor activity nervous system development cholesterol metabolic process negative regulation of peptidase activity negative regulation of endopeptidase activity positive regulation of peptidase activity positive regulation of G2/M transition of mitotic cell cycle forebrain development regulation of protein binding negative regulation of neuron differentiation neuromuscular process controlling balance synaptic growth at neuromuscular junction neuron apoptotic process smooth endoplasmic reticulum calcium ion homeostasis
          It has to be done as per old AB suggested Products section.

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