Halt™ 蛋白酶抑制剂混合物 (100X)
Halt™ 蛋白酶抑制剂混合物 (100X)
引用和文献 (4)
Thermo Scientific™

Halt™ 蛋白酶抑制剂混合物 (100X)

Thermo Scientific Halt 蛋白酶抑制剂混合物 (100X) 是一种即用型蛋白酶抑制剂浓缩储备液，可添加至样品中以防止细胞裂解和蛋白提取期间的蛋白水解降解。Halt 蛋白酶抑制剂混合物 (100X) 的特点：•便利—冰箱冷藏稳定的了解更多信息
Have Questions?
更改视图buttonViewtableView
货号数量
784295 mL
7843024 x 100μL
7843810 mL
877861 mL
货号 78429
价格（CNY)
6,258.00
Each
添加至购物车
数量:
5 mL
请求批量或定制报价
价格（CNY)
6,258.00
Each
添加至购物车
Thermo Scientific Halt 蛋白酶抑制剂混合物 (100X) 是一种即用型蛋白酶抑制剂浓缩储备液，可添加至样品中以防止细胞裂解和蛋白提取期间的蛋白水解降解。

Halt 蛋白酶抑制剂混合物 (100X) 的特点：

•便利—冰箱冷藏稳定的 100X 液体剂型比单独的抑制剂或其他规格更有效和更易于使用；只需准确量取您所需量即可
•无专有原料—完全公开的配方含有优化浓度的在高质量二甲亚砜 (DMSO) 中稳定的六种广谱蛋白酶抑制剂 AEBSF、抑肽酶、苯丁抑制素、E-64、亮肽素和抑胃肽 A
•多种包装尺寸—可选择多种包装尺寸，包括可保持蛋白酶抑制剂新鲜并降低试剂污染风险的单次使用微管
•常规配方—包括用于可选金属蛋白酶抑制的单独小瓶装 EDTA 溶液
•裂解缓冲液兼容—与 Thermo Scientific Pierce 细胞裂解缓冲液或几乎任何其他市售或自制基于去污剂的裂解试剂配合使用。添加 10 µL 浓缩混合物/1 mL 裂解缓冲液以确保完全保护所得蛋白提取物。

应用：
•保护完整的活性细胞蛋白免受内源性蛋白酶的降解
•抑制由多种方法产生的裂解液的蛋白酶活性，包括 Thermo Scientific Pierce 细胞裂解试剂、其他商业配制的细胞或组织裂解产物、超声处理、法压壶、混合器均质化或重复冻融循环
•提取物蛋白水解活性筛查
•研究细胞过程调节中的蛋白水解

蛋白酶构成了一大类酶，包括在多种细胞和组织类型中催化蛋白水解分解成肽或氨基酸的肽链内切酶和肽链外切酶。在活细胞中调节并划分各种蛋白酶，但细胞裂解将其释放并与其他抽提的蛋白混合。因此他们的活性会导致大量有价值蛋白的损失，对下游应用造成负面影响。因此，常见的做法是用已知的化合物混合物穿刺细胞裂解缓冲液或细胞抽提物以灭活或抑制各种蛋白酶。Halt 蛋白酶抑制剂混合物可有效抑制细胞裂解物样品中通常存在的丝氨酸蛋白酶、半胱氨酸蛋白酶、天冬氨酸蛋白酶和氨基肽酶。常规和无 EDTA 配方可分别抑制各种金属蛋白酶和兼容 2D 电泳。

相关产品
Halt™ 蛋白酶抑制剂单次使用混合物无 EDTA (100X)
仅供科研使用。不可用于诊断程序。
规格
缓冲液裂解缓冲液
抑制剂蛋白酶抑制剂
数量5 mL
试剂类型蛋白酶抑制剂
形式液体
产品线Halt
产品类型抑制剂混合物
Unit SizeEach
内容与储存
接收后，在 4°C 下储存

常见问题解答 (FAQ)

What are the compositions of the Halt Protease Inhibitor Cocktail, EDTA-Free (100X) (Cat. No. 87785) and Halt Protease Inhibitor Cocktail (100X) (Cat. No. 87786)?

Both formulations are the same and have the following composition:

What is the shelf life of Halt Protease Inhibitor Cocktail (100X)?

When stored as recommended at 4 degrees C, Halt Protease Inhibitor Cocktail (100X) is guaranteed stable for 1 year from the date of receipt.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can I use just part of the Pierce Protease Inhibitor XL Capsules, EDTA-Free and save part of the capsule for later use?

We do not recommend “splitting” the capsule into smaller portions. Researchers using less than 500 mL should choose a different format (liquid or tablet) of protease inhibitors.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What formats of protease inhibitors do you offer?

We offer standalone protease inhibitors (AEBSF, Aprotinin, PMSF, EDTA, etc.), as well as cocktails or combinations of protease inhibitors in liquid, tablet, and larger capsule formats. These cocktails of protease inhibitors can also be purchased in combination with a cocktail of phosphatase inhibitors, in both liquid and tablet form, for even greater convenience.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What protease inhibtors are present in the Halt Protease Inhibitor Single-Use Cocktail?

The inhibitors included in the Halt Protease Inhibitor Single-Use Cocktail (Cat. No. 78430) are: AEBSF, Aprotinin, Bestatin, E64, Leupeptin, Pepstatin A, and EDTA.

View all Halt™ 蛋白酶抑制剂混合物 (100X) FAQs

引用和文献 (4)

引用和文献
Abstract
Overexpression of TBX3 suppresses tumorigenesis in experimental and human cholangiocarcinoma.
Authors:Deng S,Lu X,Wang X,Liang B,Xu H,Yang D,Cui G,Yonemura A,Paine H,Zhou Y,Zhang Y,Simile MM,Urigo F,Evert M,Calvisi DF,Green BL,Chen X
Journal:Cell death & disease
PubMed ID:38909034
TBX3 behaves as a tumor suppressor or oncoprotein across cancer. However, TBX3 function remains undetermined in intrahepatic cholangiocarcinoma (iCCA), a deadly primary liver malignancy with few systemic treatment options. This study sought to investigate the impact of TBX3 on iCCA. We found that overexpression of TBX3 strongly inhibited human iCCA ... More
Diverse mechanisms of DDX3Y suppression by DDX3X.
Authors:Xu X,Wei S
Journal:bioRxiv : the preprint server for biology
PubMed ID:39975375
The DEAD-box RNA helicase DDX3X has important roles in development and disease. Loss of DDX3X during developmental and pathological processes such as tumorigenesis can lead to compensatory upregulation of the close paralog DDX3Y in males, which may underlie the sexual dimorphism displayed by some DDX3X-associated diseases. However, how DDX3X cross-regulates ... More
FOXO1 modulates osteoblast differentiation.
Authors:Siqueira MF,Flowers S,Bhattacharya R,Faibish D,Behl Y,Kotton DN,Gerstenfeld L,Moran E,Graves DT
Journal:Bone
PubMed ID:21281751
Forkhead box O1 (FOXO1) is upregulated during bone formation and in response to stimulation by bone morphogenetic proteins. Studies presented here examined the functional role of FOXO1 in a well defined culture system in which pre-osteoblastic cells undergo terminal differentiation in vitro. Mineralizing cultures of MC3T3-E1 cells were examined with ... More
Glucagon-like peptide 1 inhibits cell apoptosis and improves glucose responsiveness of freshly isolated human islets.
Authors:Farilla L, Bulotta A, Hirshberg B, Li Calzi S, Khoury N, Noushmehr H, Bertolotto C, Di Mario U, Harlan DM, Perfetti R
Journal:Endocrinology
PubMed ID:12960095
The peptide hormone, glucagon-like peptide 1 (GLP-1), has been shown to increase glucose-dependent insulin secretion, enhance insulin gene transcription, expand islet cell mass, and inhibit beta-cell apoptosis in animal models of diabetes. The aim of the present study was to evaluate whether GLP-1 could improve function and inhibit apoptosis in ... More