Pierce™ IP 裂解缓冲液
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Pierce™ IP 裂解缓冲液
Thermo Scientific™

Pierce™ IP 裂解缓冲液

Thermo Scientific Pierce IP 裂解缓冲液针对细胞裂解物的产量、纯度以及与免疫沉淀(IP 和 Co-IP了解更多信息
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货号数量
87787100 mL
87788250 mL
货号 87787
价格(CNY)
938.00
飞享价
Ends: 31-Dec-2025
1,368.00
共减 430.00 (31%)
Each
添加至购物车
数量:
100 mL
请求批量或定制报价
价格(CNY)
938.00
飞享价
Ends: 31-Dec-2025
1,368.00
共减 430.00 (31%)
Each
添加至购物车
Thermo Scientific Pierce IP 裂解缓冲液针对细胞裂解物的产量、纯度以及与免疫沉淀(IP 和 Co-IP)(作为与细胞裂解物相关的下游应用)的兼容性进行了优化。

IP 裂解缓冲液的特点:

•经过优化,可与各种免疫沉淀和牵出测定兼容
• 与各种蛋白测定、报告基因测定和免疫检测程序兼容
• 现成的配方,可有效提取细胞质、细胞膜和细胞核蛋白
• 温和的配方可帮助保持蛋白复合物用于免疫共沉淀
• 不会释放可导致样品高粘性的基因组 DNA

IP 裂解缓冲液是一种基于改良 RIPA 缓冲液配方(不含 SDS)的哺乳动物全细胞裂解试剂。这种中等强度裂解缓冲液可高效溶解细胞蛋白,但不会像一般 RIPA 缓冲液那样释放染色体组 DNA 或破坏蛋白复合物。Pierce IP 裂解缓冲液经过特殊配方,可用于各种牵出测定和免疫沉淀检测。

Pierce IP 裂解缓冲液可从孔板细胞和经悬浮培养液粒化的细胞中有效裂解出培养的哺乳动物细胞。该裂解缓冲液经过优化可用于各种牵出测定和免疫沉淀检测,还可与很多其他应用兼容,包括 Thermo Scientific Pierce BCA 和 660 nm 蛋白测定、蛋白纯化和各种免疫检测(如 ELISA、Western 印迹)。

Pierce IP 裂解缓冲液由 25 mM Tris-HCl pH 7.4、150 mM NaCl、1 mM EDTA、1% NP-40 和 5% 甘油组成。该缓冲液不含蛋白酶或磷酸酶抑制剂;但是如果需要,可在使用前添加各种抑制剂,如 Thermo Scientific Halt 蛋白酶抑制剂混合物或磷酸酶抑制剂混合物,以防止蛋白水解并保持蛋白磷酸化。

相关产品
M-PER™ 哺乳动物蛋白提取试剂
RIPA 裂解和提取缓冲液
仅供科研使用。不可用于诊断程序。
规格
产品规格液体
数量100 mL
容积(公制)100 mL
产品线Pierce™
产品类型IP 裂解液
Unit SizeEach

常见问题解答 (FAQ)

What are the standard lysis buffers used with mammalian cells for detection of protein expression by immunoprecipitation (IP) or Western blot analysis?

The most commonly used buffer is RIPA Buffer with SDS. We offer RIPA Buffer (Cat. Nos. 89900 and 89901). We also offer the Pierce IP Lysis buffer (Cat. Nos. 87787 and 87788) as well as M-PER (Cat. Nos. 78501, 78503, and 78505).

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

Can you provide the shelf-life for the Pierce IP Lysis Buffer?

The Pierce IP Lysis Buffer is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can you provide the shelf life for Pierce IP Lysis Buffer?

Pierce IP Lysis Buffer is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended (4 degrees C). Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can I store Pierce IP Lysis Buffer (Cat. No. 87787) at room temperature?

Pierce IP Lysis Buffer ships at ambient temperature and can be stored at room temperature. However, we recommend 4 degrees C for long term storage in order to prevent possible bacterial growth.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Which protein assays are compatible with the Thermo Scientific IP Lysis Buffer?

The Thermo Scientific IP Lysis Buffer is compatible with Thermo Scientific BCA Protein Assay (Cat. No. 23225) and Thermo Scientific 660 nm Protein Assay (Cat. No. 22660). It is not compatible with Thermo Scientific Coomassie Plus (Bradford) Assay (Cat. No. 23236) unless diluted 1:1 in TBS, PBS, or water.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.