RIPA 裂解和提取缓冲液
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RIPA 裂解和提取缓冲液
Thermo Scientific™

RIPA 裂解和提取缓冲液

Thermo Scientific RIPA 裂解和提取缓冲液是一种用于培养的哺乳动物细胞的常用细胞裂解试剂的高质量、即用型且完全披露的配方。RIPA 缓冲液的特点：•便利— 即用型解决方案；无需自行装配和制备组成部分•灵活— 可与许多应用兼容，包括报告基因检测、蛋白检测了解更多信息
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货号数量
89901250 mL
89900100 mL
货号 89901
价格（CNY)
1,368.00
飞享价
Ends: 31-Dec-2025
1,851.00
共减 483.00 (26%)
250 mL
添加至购物车
数量:
250 mL
请求批量或定制报价
价格（CNY)
1,368.00
飞享价
Ends: 31-Dec-2025
1,851.00
共减 483.00 (26%)
250 mL
添加至购物车
Thermo Scientific RIPA 裂解和提取缓冲液是一种用于培养的哺乳动物细胞的常用细胞裂解试剂的高质量、即用型且完全披露的配方。

RIPA 缓冲液的特点：

•便利— 即用型解决方案；无需自行装配和制备组成部分
•灵活— 可与许多应用兼容，包括报告基因检测、蛋白检测、免疫检测和蛋白纯化
•通用— 可提取细胞质、细胞膜和细胞核蛋白
•披露的配方— 不含专有组成部分，用户可全面控制和了解可能存在的兼容性问题

该 RIPA 缓冲液可从培养的哺乳动物细胞（包括铺板细胞和团状悬浮细胞）中高效裂解并提取蛋白。该常用试剂可提取细胞膜、细胞核和细胞质蛋白，并可与许多应用兼容，包括报告基因检测、Thermo Scientific BCA 蛋白检测、免疫检测和蛋白纯化。Thermo Scientific Halt 蛋白酶抑制剂混合物（产品编号 78430）和 Halt 磷酸酶抑制剂混合物（产品编号 78420）等抑制剂也可与该 RIPA 缓冲液配方兼容，可在使用前添加以防止蛋白水解并保持蛋白磷酸化。

RIPA 缓冲液的名称来源于为其开发这种缓冲液的原始应用：放射免疫沉淀测定。尽管目前在实验室中很少使用这种同位素检测方法，但该裂解缓冲液配方的首字母缩略词已被广泛使用。RIPA 细胞裂解试剂可从各种细胞类型中高效提取蛋白，因为其含有三种非离子型和离子型去污剂。该去污剂配方的一大劣势是，与其他裂解试剂相比，其与某些下游应用相对不兼容。

相关产品
Pierce™ IP 裂解缓冲液
M-PER™ 哺乳动物蛋白提取试剂
仅供科研使用。不可用于诊断程序。
规格
产品规格液体
数量250 mL
容积（公制）250 mL
产品类型提取缓冲液
Unit Size250 mL
内容与储存
接收后，在 4°C 下储存。

常见问题解答 (FAQ)

What are the standard lysis buffers used with mammalian cells for detection of protein expression by immunoprecipitation (IP) or Western blot analysis?

The most commonly used buffer is RIPA Buffer with SDS. We offer RIPA Buffer (Cat. Nos. 89900 and 89901). We also offer the Pierce IP Lysis buffer (Cat. Nos. 87787 and 87788) as well as M-PER (Cat. Nos. 78501, 78503, and 78505).

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

Does RIPA Lysis and Extraction Buffer (Cat. No. 89900, 89901) contain protease or phosphatase inhibitors?

RIPA Lysis and Extraction Buffer (Cat. No. 89900, 89901) does not contain protease or phosphatase inhibitors. If desired, you may add protease and/or phosphatase inhibitors, such as Halt Protease Inhibitor Cocktail (Cat. No. 78410) and Halt Phosphatase Inhibitor Cocktail (Cat. No. 78420) to the RIPA Lysis and Extraction Buffer to prevent proteolysis and maintain phosphorylation status of proteins. We recommend adding protease and phosphatase inhibitors immediately before use.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Why is it not recommended that I use RIPA buffer for protein A280 measurements with my NanoDrop spectrophotometer?

RIPA buffer produces a particularly strong absorbance signal at the 280 nm wavelength. As a result, it will either over estimate or under estimate protein concentrations and interfere with the protein purity ratio.
Protein samples in RIPA buffer should be quantified via the Pierce Protein 660 or BCA colorimetric assays using a full spectrum NanoDrop model.

Find additional tips, troubleshooting help, and resources within ourProtein Purification and Isolation Support Center.

Why is there low phosphorylation of the proteins when I use the RIPA Lysis and Extraction Buffer?

Low phosphorylation is usually due to phosphatase activity. We recommend adding a Halt Phosphatase Inhibitor Cocktail to the buffer before use.
Alternatively, the protein is not phosphorylated or phosphorylated at a low level.

Find additional tips, troubleshooting help, and resources within our Cell Lysis and Fractionation Support Center.

What if proteolysis occurs when I use RIPA Lysis and Extraction Buffer?

Proteolysis indicates that no protease inhibitors were added. We recommend adding a Halt Protease Inhibitor Cocktail to the RIPA Lysis and Extraction Buffer before use.

Find additional tips, troubleshooting help, and resources within ourProtein Purification and Isolation Support Center.

View all RIPA 裂解和提取缓冲液 FAQs