The RNA helicase Dbp10 coordinates assembly factor association with PTC maturation during ribosome biogenesis.
AuthorsMitterer V,Hamze H,Kunowska N,Stelzl U,Henras AK,Hurt E
JournalNucleic acids research
PubMed ID38113283
During ribosome biogenesis a plethora of assembly factors and essential enzymes drive the unidirectional maturation of nascent pre-ribosomal subunits. The DEAD-box RNA helicase Dbp10 is suggested to restructure pre-ribosomal rRNA of the evolving peptidyl-transferase center (PTC) on nucleolar ribosomal 60S assembly intermediates. Here, we show that point mutations within conserved ... More
Enantioselective OTUD7B fragment discovery through chemoproteomics screening and high-throughput optimisation.
AuthorsVuorinen A,Kennedy CR,McPhie KA,McCarthy W,Pettinger J,Skehel JM,House D,Bush JT,Rittinger K
JournalCommunications chemistry
PubMed ID39809917
Deubiquitinating enzymes (DUBs) are key regulators of cellular homoeostasis, and their dysregulation is associated with several human diseases. The ovarian tumour protease (OTU) family of DUBs are biochemically well-characterised and of therapeutic interest, yet only a few tool compounds exist to study their cellular function and therapeutic potential. Here we ... More
Proteomic analysis of Neobenedenia sp. and Rhabdosynochus viridisi (Monogenea, Monopisthocotylea): Insights into potential vaccine targets and diagnostic markers for finfish aquaculture.
Monogeneans are parasitic flatworms that represent a significant threat to the aquaculture industry. Species like Neobenedenia melleni (Capsalidae) and Rhabdosynochus viridisi (Diplectanidae) have been identified as causing diseases in farmed fish. In the past years, molecular research on monogeneans of the subclass Monopisthocotylea has focused on the generation of genomic ... More
Transformation of hempseed (Cannabis sativa L.) oil cake proteome, structure and functionality after extrusion.
AuthorsLeonard W,Zhang P,Ying D,Nie S,Tindal E,Fang Z
JournalFood chemistry
PubMed ID35193020
The entire protein fractions from hempseed, its oil cake (30-40% protein) and the extruded protein isolate (>90% protein) were investigated. The first semi-quantitative mass spectrometry-based proteomics on hempseed was performed, leading to a sum of 1879 differentially abundant proteins being identified from individual pairwise comparisons of each extruded group compared ... More
Identification of permissive amber suppression sites for efficient non-canonical amino acid incorporation in mammalian cells.
AuthorsBartoschek MD,Ugur E,Nguyen TA,Rodschinka G,Wierer M,Lang K,Bultmann S
JournalNucleic acids research
PubMed ID33684219
The genetic code of mammalian cells can be expanded to allow the incorporation of non-canonical amino acids (ncAAs) by suppressing in-frame amber stop codons (UAG) with an orthogonal pyrrolysyl-tRNA synthetase (PylRS)/tRNAPylCUA (PylT) pair. However, the feasibility of this approach is substantially hampered by unpredictable variations in incorporation efficiencies at different ... More
Discerning the Primary Carcinoma in Malignant Peritoneal and Pleural Effusions Using Imaging Mass Spectrometry-A Feasibility Study.
PURPOSE: Malignant effusions challenge diagnostic accuracy due to cytomorphologic overlaps between various malignant primaries. Workup of this material to establish a correct diagnosis is time consuming and limited by the sparsity of material. In order to circumvent these drawbacks, the use of MALDI imaging MS (IMS) as a diagnostic platform ... More
Multi-attribute method (MAM) to assess analytical comparability of adalimumab biosimilars.
AuthorsMillán-Martín S,Jakes C,Carillo S,Bones J
JournalJournal of pharmaceutical and biomedical analysis
PubMed ID37385093
Adalimumab drug product (Humira ®), the first fully human monoclonal antibody (mAb) approved by FDA in 2002, led the top ten list of best-selling mAbs in 2018 and has been the most profitable drug in the world. With the expiration of patent protection in Europe in 2018 and in United ... More
A metabolite sensor subunit of the Atg1/ULK complex regulates selective autophagy.
AuthorsGross AS,Ghillebert R,Schuetter M,Reinartz E,Rowland A,Bishop BC,Stumpe M,Dengjel J,Graef M
JournalNature cell biology
PubMed ID38316984
Cells convert complex metabolic information into stress-adapted autophagy responses. Canonically, multilayered protein kinase networks converge on the conserved Atg1/ULK kinase complex (AKC) to induce non-selective and selective forms of autophagy in response to metabolic changes. Here we show that, upon phosphate starvation, the metabolite sensor Pho81 interacts with the adaptor ... More
Studies on the subunit structure of textilotoxin, a potent presynaptic neurotoxin from the venom of the Australian common brown snake (Pseudonaja textilis). 2. The amino acid sequence and toxicity studies of subunit D.
AuthorsPearson JA,Tyler MI,Retson KV,Howden ME
JournalBiochimica et biophysica acta
PubMed ID2015288
Textilotoxin is a presynaptic neurotoxin in the venom of the Australian common brown snake, Pseudonaja textilis. It has the highest lethality and is structurally the most complex of any known snake venom neurotoxin. Reverse-phase HPLC was used to resolve textilotoxin into subunits A, B, C and D. Subunit D consists ... More
Characterization of Physical, Mechanical, and Biological Properties of SilkBridge Nerve Conduit after Enzymatic Hydrolysis.
The in vitro degradation profile and the cytotoxicity of the degradation products of a silk fibroin (SF)-based nerve conduit (SilkBridge), with a complex three-layered wall architecture comprising both native and regenerated (electrospun) fibers, are reported. The bacterial protease type XIV from Streptomyces griseus was used as a hydrolytic agent at ... More