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CytoScan™ XON Assay Kit - FAQs

CytoScan™ XON Assay Kit - FAQs

查看更多产品信息 CytoScan™ XON Assay Kit - FAQs (931311)

9 个常见问题解答

Is the CytoScan XON Assay (Cat. No. 931311, 931312) validated for FFPE samples?

No, the CytoScan XON Assay has only been validated for blood samples.

Does Ref103 come in the CytoScan XON Assay Kit (Cat. No. 931311)?

Reference Genomic DNA 103 is not included in the kit, but is available for purchase separately (Cat. No. 901012).

Note: A positive control is recommended for the CytoScan XON assay.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Can the CytoScan XON assay differentiate between a gene and pseudogene by copy number analysis at the exon level?

Unfortunately, no, it is not possible to map to one or the other.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Has the CytoScan XON rehybridization assay been tested and verified?

Yes, the CytoScan XON rehybridization assay has been tested and the hybridization cocktail can be saved.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Which version of Chromosome Analysis Suite (ChAS) Software is required to analyze CytoScan XON arrays?

Chromosome Analysis Suite (ChAS) Software version 3.3 is required to analyze CytoScan XON arrays. 

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

Are the equipment requirements for running the CytoScan XON assay different from the CytoScan assay?

Consumable and equipment requirements are the same for running both the CytoScan XON assay and the CytoScan assay.   

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What are the quality requirements for genomic DNA for a successful run on a CytoScan XON array?

Sample must be double-stranded genomic DNA (gDNA) that is not degraded (size 10 kb by gel analysis), not contaminated, and free of PCR inhibitors. Sample should be diluted to 20 ng/µL in low-EDTA TE buffer. 

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.

What is the Median Absolute Pairwise Difference (MAPD) metric?

MAPD is the recommended method of assessing noise in the SNP Array 6.0, Cytoscan HD and Cytoscan DX copy number data. The log2 distance between neighbors is calculated for all pairs and then the median is taken. This represents the typical distance between marker pairs with respect to log2 ratios. The higher the MAPD, the greater the noise between the array of interest and the reference set.

Find additional tips, troubleshooting help, and resources within our Microarray Analysis Support Center.