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Citations & References (13)
Gibco™
StemPro™ MSC SFM
StemPro™ MSC SFM is a serum-free medium (SFM) specially formulated for the growth and expansion of human mesenchymal stem cells (MSCs).
| Catalog Number | With Additives | Without Additives |
|---|---|---|
| A1033201 | Low Glucose, Sodium Pyruvate, Sodium Bicarbonate, Adenosine, Deoxyribonucleosides | No Glutamine, No Phenol Red |
Catalog number A1033201
Price (CNY)
4,239.00
Each
With Additives:
Low Glucose, Sodium Pyruvate, Sodium Bicarbonate, Adenosine, Deoxyribonucleosides
Without Additives:
No Glutamine, No Phenol Red
Price (CNY)
4,239.00
Each
StemPro™ MSC SFM is a serum-free medium (SFM) specially formulated for the growth and expansion of human mesenchymal stem cells (MSCs). StemPro™ MSC SFM enables superior human MSC growth and increased consistency compared to classical serum-supplemented medium (DMEM + 10% FBS).
Using StemPro™ MSC SFM, human MSCs can be expanded beyond 5 passages while still maintaining their tri-lineage mesoderm differentiation potential (i.e., ability to differentiate into osteogenic, chondrogenic and adipogenic lineages). Each lot of StemPro™ MSC SFM is qualified using a human MSC performance assay and human MSCs growing in serum-supplemented medium can be transitioned directly into StemPro™ MSC SFM with little or no adaptation required. In addition, StemPro™ MSC SFM facilitates expansion of MSCs directly from primary human bone marrow.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMesenchymal Stem Cells
Culture EnvironmentCO2
Endotoxin LevelLow (≤10 EU/mL)
Inorganic SaltsCalcium, Sodium Phosphate
Product LineStemPro
Product TypeStem Cell Serum Free Media (SFM)
Purity or Quality GradeResearch Grade
Quantity1 Kit
SpeciesHuman
ClassificationSerum-free
Culture TypeAdherent Cell Culture
FormLiquid
Serum LevelSerum-free
With AdditivesLow Glucose, Sodium Pyruvate, Sodium Bicarbonate, Adenosine, Deoxyribonucleosides
Without AdditivesNo Glutamine, No Phenol Red
Unit SizeEach
Contents & Storage
This kit is shipped in two parts with separate storage requirements. These components are not sold individually:
• StemPro™ MSC SFM Basal Medium, 500 mL (ships at room temperature, store in the dark at 2°C to 8°C)
• StemPro™ MSC SFM Supplement, 75 mL (ships on dry ice, store in the dark at -5°C to -20°C)
• StemPro™ MSC SFM Basal Medium, 500 mL (ships at room temperature, store in the dark at 2°C to 8°C)
• StemPro™ MSC SFM Supplement, 75 mL (ships on dry ice, store in the dark at -5°C to -20°C)
Frequently asked questions (FAQs)
Can StemPro MSC SFM (Cat. No. A1003201) be used to isolate primary cells?
Do StemPro MSC SFM Basal Medium CTS and StemPro MSC SFM Supplement CTS contain L-Glutamine?
What kinds of growth factors are used in mesenchymal stem cell (MSC) research?
How can I culture mesenchymal stem cells (MSCs) in vitro?
How do you characterize human mesenchymal stem cells (MSCs)?
Citations & References (13)
Citations & References
Abstract
PDGF, TGF-beta, and FGF signaling is important for differentiation and growth of mesenchymal stem cells (MSCs): transcriptional profiling can identify markers and signaling pathways important in differentiation of MSCs into adipogenic, chondrogenic, and osteogenic lineages.
Journal:Blood
PubMed ID:18332228
We compared the transcriptomes of marrow-derived mesenchymal stem cells (MSCs) with differentiated adipocytes, osteocytes, and chondrocytes derived from these MSCs. Using global gene-expression profiling arrays to detect RNA transcripts, we have identified markers that are specific for MSCs and their differentiated progeny. Further, we have also identified pathways that MSCs
Ischemic Preconditioning Potentiates the Protective Effect of Stem Cells through Secretion of Exosomes by Targeting Mecp2 via miR-22.
Journal:
PubMed ID:24558412
Mesenchymal stem cells (MSCs) have potential application for the treatment of ischemic heart diseases. Besides differentiation properties, MSCs protect ischemic cardiomyocytes by secretion of paracrine factors. In this study, we found exosomes enriched with miR-22 were secreted by MSCs following ischemic preconditioning (Exo(IPC)) and mobilized to cardiomyocytes where they reduced
Critical steps in the isolation and expansion of adipose-derived stem cells for translational therapy.
Journal:
PubMed ID:26052798
Since the discovery of adipose-derived stem cells (ASCs), there have been high expectations of their putative clinical use. Recent advances support these expectations, and it is expected that the transition from pre-clinical and clinical studies to implementation as a standard treatment modality is imminent. However ASCs must be isolated and
Serum-free, xeno-free culture media maintain the proliferation rate and multipotentiality of adipose stem cells in vitro.
Journal:Cytotherapy
PubMed ID:19903107
BACKGROUND AIMS: Human adipose stem cells (ASC) are an abundant, readily available population of multipotent progenitor cells that reside in adipose tissue. ASC have been shown to have therapeutic applicability in pre-clinical studies, but a standardized expansion method for clinical cell therapy has yet to be established. Isolated ASC are
Growth, differentiation capacity, and function of mesenchymal stem cells expanded in serum-free medium developed via combinatorial screening.
Journal:Exp Cell Res
PubMed ID:23597555
The presence of serum in cell culture medium presents an obstacle to safe and efficient production of hMSCs for therapeutic purposes. Availability of defined medium will be crucial to elucidating the mechanism of action of hMSCs in many indications as well as a prerequisite to consistently produce cells with predictable