How stable is AlgiMatrix Matrix at room temperature without degradation?
AlgiMatrix Matrix is stable for 12 months unopened. Once opened, the entire plate needs to be used.
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Can other cell viability reagents be used with AlgiMatrix Matrix?
Other than the Invitrogen alamarBlue Cell Viability Reagent, AlgiMatrix Matrix is also compatible with Invitrogen PrestoBlue Cell Viability Reagent and Invitrogen CyQuant Direct Cell Proliferation Assay. The protocol is almost the same as the 2D cell culture, but it needs the blank AlgiMatrix Matrix as a blank control.
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How important is calcium in the AlgiMatrix firming and dissolving buffers?
The firming buffer works by adding calcium to the sponge, making it stiffer. The dissolving buffer works by removing calcium from the sponge, making it softer.
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Is there any assay variability when using AlgiMatrix Matrix?
3D cell culture with AlgiMatrix Matrix approaches a more physiological state in terms of cell growth and behavior, but along with that comes the higher variability typically associated with more complex systems. You may need to do more replicates or more controls.
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Can I use AlgiMatrix Matrix for primary cells?
Some primary cells (with the exception of stem cells) need to adhere in order to expand. As cells won't adhere to AlgiMatrix Matrix, these cells can still be cultured, but will tend to differentiate rather than expand. We recommend that you plate at a high cell density for primary cells.
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