96-well, Collagen Type I-Coated, Flat-Bottom Microplates - FAQs

We suggest the following alternatives for 96-well, Collagen Type I-Coated, Flat-Bottom Microplates (Cat. No. A1142803):

96 Well Plate, Collagen I Coated Surface, Pack of 5 (Cat. No. 152038)

96 Well Black/Clear Bottom Plate, Collagen I Coated Surface, Pack of 5 (Cat. No. 152036)

Nunc MicroWell 96-Well, Collagen Type I-Treated, Flat-Bottom, Optical Polymer Base Microplate (Cat. No. 152040)

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Enzymes that can be used include: Gibco TrypLE Express Enzyme, Gibco TrypLE Select Enzyme, or Gibco StemPro Accutase Cell Dissociation Reagent.

Find additional tips, troubleshooting help, and resources within our Extracellular Matrices (ECM) Support Center.

The following cell types are commonly cultured in collagen I:

- Endothelial cells (including HUVEC)
- Fibroblasts
- Hepatocytes
- Chondrocytes
- Osteoclasts
- Osteoblasts
- Keratinocytes
- Corneal epithelium

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

The key applications include the following:

- Collagen I induces microvascular endothelial cells to adopt a spindle-shaped morphology, in vitro, and to align into solid cord-like assemblies. Vascular endothelial cells can also form vessel-like, tubular structures when cultured on collagen I. Collagen I can be used for in vitro angiogenesis assays.
- Breast cancer stem cells can undergo differentiation when cultured on collagen I.
- Collagen I has been used for the culture of primary colon carcinoma cell lines; mouse liver progenitor cells have been cultured in 3D collagen I and rat pancreatic islets.
- Collagen I can act as a barrier in cell invasion assays, and has been used to study cell adhesion.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Gibco Geltrex Matrix and collagen (rat tail and bovine) can be used as either a coating solution or a 3D gel matrix. CELLstart Substrate was developed to be used as a xeno-free coating matrix for only ESC applications (as a substitute for Gibco Geltrex Matrix or Matrigel Matrix, which are of animal origin). AlgiMatrix Matrix is a 3D scaffold-type matrix that does not support cell attachment but does provide a good environment for growing spheroids that can be easily harvested for downstream applications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.