Search
Search
查看更多产品信息 Episomal iPSC Reprogramming Vectors - FAQs (A14703)
6 个常见问题解答
我们提供Invitrogen CytoTune-iPS 2.0仙台病毒重编程试剂盒（Invitrogen CytoTune-iPS 2.0 Sendai Reprogramming Kit，货号A16517，A16518），这是一套非整合型的系统，通过仙台病毒载体将体细胞重编程为诱导性多能干细胞（iPSCs）。Invitrogen CytoTune-iPS 2.0仙台病毒重编程试剂盒包含了三种用于递送和表达关键遗传因子的Invitrogen CytoTune 2.0重编程载体，这些因子的表达对于将体细胞重编程为iPSCs的操作是必需的。只需要一次应用这些载体即可成功的实现重编程。
此外，我们还提供附着体iPSC重编程载体 (货号A14703) 或 Epi5附着体iPSC重编程试剂盒 (货号 A15960)。其同样是非整合型系统能将体细胞重编程为诱导性多能干细胞（iPSCs）。本品是六种或五种载体的混合物，为在无饲养层环境下生成iPSC提供优化的体系，具有非病毒介导、无基因组整合的特点。这些附着体iPSC重编程载体最初由Junying Yu与James Thomson开发（http://www.ncbi.nlm.nih.gov/pubmed/19325077），并经过Cellular Dynamics International的进一步优化，能够胜任大量不同种类的体细胞的重编程操作。
人iPS与人ES细胞的生长条件相同。与人ES细胞类似，人iPS细胞能够生长于辐照的MEF滋养层细胞上+含20% KnockOut SR，0.1 mM NEAA，1mM谷氨酰胺，0.1mM b-ME的DMEM/F12培养基 + 辐照MEF饲养细胞 + 100 ng/mL bFGF的培养条件中，或Matrigel基质包被培养皿 + MEF条件培养基 + 100 ng/mL bFGF的培养条件中（Science318:1917 (2007)）。人ES细胞与iPS细胞两者均可生长于KnockOut ESC/iPS培养基中（货号A14131）。
用于细胞培养：人重组激活化素A（货号PHC9564），bFGF（货号PHG0261），IGF-II（货号PHG0084）（Stem Cells 24:1476 (2006); Nature 448:1015 (2007)）。
用于人ES细胞分化：BMP-4（货号PHC9533），EGF（货号PHG0311）和HGF（货号PHG0324）（Proc Natl Acad Sci U S A 97:11307 (2000)）。
We offer the Invitrogen CytoTune-iPS 2.0 Sendai Reprogramming Kit (Cat. Nos. A16517, A16518) which is a non-integrating system that uses Sendai virus vectors to reprogram somatic cells into induced pluripotent stem cells (iPSCs). The Invitrogen CytoTune-iPS 2.0 Sendai Reprogramming Kit contains three Invitrogen CytoTune 2.0 reprogramming vectors that are used for delivering and expressing key genetic factors necessary for reprogramming somatic cells into iPSCs. Only one application of the vectors is required for successful reprogramming.
Additionally, we offer the Episomal iPSC Reprogramming Vectors (Cat. No A14703) or Epi5 Episomal iPSC Reprogramming Kit (Cat. No A15960). This is a non-integrating system that reprograms somatic cells into induced pluripotent stem cells (iPSCs). These two products contain a mixture of six or five vectors designed to provide the optimal system for generating transgene-free and virus-free iPSCs in a feeder-free environment. Originally developed by Junying Yu and James Thomson (http://www.ncbi.nlm.nih.gov/pubmed/19325077) and further optimized by Cellular Dynamics International, these Episomal iPSC Reprogramming Vectors have proven successful in reprogramming a number of different somatic cell types.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
The full length Human FGF-basic (FGF-2/bFGF) (aa 1-155) Recombinant Protein is recommended for stem cells whereas the truncated variant, Human FGF-basic (FGF-2/bFGF) (aa 10-155) Recombinant Protein which is missing the first 9 amino acids, is recommended for use with neural and cardiac cells.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Induced pluripotent stem cells (iPS or iPSCs) are pluripotent stem cells directly generated by introducing combination of genes coding for “reprogramming factors” into adult cells. These reprogramming factors include Oct4, Sox2, c-Myc, KLF4, NANOG, and LIN28. Yu, et al, generated iPS from a human mesenchymal cell line using lentiviral vectors carrying Oct4, Sox2, NANOG, and LIN28 genes (Science 318:1917 (2007)). Using a similar approach, Takahashi et al, generated iPS from human primary fibroblast cells by introducing genes coding for Oct3, Sox2, KLF4, and c-Myc into these cells (Cell 131:861 (2007)). iPS generated by reprogramming are similar to human ES cells in morphology, the capacity for unlimited proliferation, surface-antigen expression, gene expression, the ability to differentiate into cell types representing the three germ layers in vitro, and the ability to form teratomas after injection into SCID mice.