Essential 8™ 培养基
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Essential 8™ 培养基
引用和文献 (5)
Gibco™

Essential 8™ 培养基

Essential 8™ 培养基是一种无异种成分、无饲养层的培养基，专为人多能干细胞 (PSC) 的生长和扩增而配制。Essential 8™ 培养基最初由 James了解更多信息
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货号数量
A1517001500 mL
货号 A1517001
价格（CNY)
2,109.00
飞享价
Ends: 27-Dec-2025
3,944.00
共减 1,835.00 (47%)
Each
添加至购物车
数量:
500 mL
价格（CNY)
2,109.00
飞享价
Ends: 27-Dec-2025
3,944.00
共减 1,835.00 (47%)
Each
添加至购物车
Essential 8™ 培养基是一种无异种成分、无饲养层的培养基，专为人多能干细胞 (PSC) 的生长和扩增而配制。Essential 8™ 培养基最初由 James Thomson 实验室的 Guokai Chen 等人所开发（以“E8”命名发表）并经 Cellular Dynamics International 所验证，目前已经得到了广泛的测试并证明可维持多种 iPSC 细胞系的多能性。此外，Essential 8™ 培养基已经被用来提高 iPSC 的产量，而且有证据表明其支持 iPSC 生长 >50 代而没有任何核型异常的迹象，同时还保持了 iPSC 分化为全部三系生殖谱系的能力。

Essential 8™ 培养基的特点包括：

•一致 — 与现有的无饲养层培养基相比减少了差异性
• 稳健 — 通过无异种成分、cGMP、8-成分培养基实现稳健可靠的培养
• 高性价比 — 与其他无饲养层培养基相比，经济且可扩展 PSC 培养

变异性更低
Essential 8™ 培养基为无异种培养基，只包含 8 种干细胞培养所需的成分。与其他包含 20 多种高度可变成分的培养基不同，Essential 8™ 培养基按照 cGMP 的要求生产，具有经过优化的配方和生长因子水平，有助于确保最大程度的细胞健康、多能性和生长率，且差异性极低。

稳健可靠的培养
与其他饲养系统相比，Essential 8™ 培养基已被证明可支持多能干细胞生长，并且能为培养提供出色的形态和生长动力学。

高性价比
Essential 8™ 培养基以一种便捷的双组分试剂盒（500 mL 基础培养基& 10 mL 补充剂）提供，当配合玻连蛋白 (VTN-N) 一起使用时，成份明确的人多能干细胞 (PSC) 无饲养层培养系统是符合成本效益的。

Essential 8™ 培养基与 Cellular Dynamics International 合作实现商业化。
仅供科研使用。不可用于诊断程序。
规格
细胞系Embryonic Stem Cells (ESCs), Induced Pluripotent Stem Cells (iPSCs)
细胞类型胚胎干细胞 (ESC)、诱导多能干细胞 (iPSC)
最大浓度Essential 8 Basal Medium (1X), Essential 8 Supplement (50X)
生产质量符合医疗器械 cGMP（21 CFR 第 820 部分）和 ISO 13485
产品线Essential 8
产品类型干细胞培养基
数量500 mL
有效期12 个月
运输条件补充剂 - 干冰，基础 - 环境条件
种属人
分类无异种成分
培养类型无饲养层、干细胞（人、iPS - 诱导多能干细胞、胚胎）, Feeder-free Stem Cell Culture (Human, iPS - Induced Pluripotent Stem, Embryonic), Stem Cell (Human, iPS - Induced Pluripotent Stem, Embryonic)
形式液体
血清水平无血清
无菌Sterile-filtered
灭菌方法Sterile-filtered
加有添加剂酚红
Unit SizeEach
内容与储存
• 500 mL 基础培养基（储存在 2—8°C 下并避光）
• 10 mL 添加剂（储存在 -5 至 -20°C 下并避光）

常见问题解答 (FAQ)

之前冻存于其他培养条件的多能干细胞（PSCs）能复苏并后续培养于Gibco Essential 8培养基+玻连蛋白（VTN-N）的组合条件中么？

可以。在mTeSR培养基+BD Matrigel基底膜基质条件下培养并冻存的PSCs细胞可复苏至Gibco Essential 8培养基中并种植于VTN-N基质上。某些细胞系复苏至冻存前的生长培养基及基质中可能会效果更好。在随后的下一次传代过程中，就可使用EDTA将这些细胞传代至Gibco Essential 8培养基+VTN-N的组合中了。

有哪些细胞系在无饲养层培养条件下通过了Gibco Essential 8培养基的测试？

有多株多能干细胞（PSC）系通过了Gibco Essential 8培养基的测试（Chen G, Gulbranson DR, Hou Z et al (2011) Chemically defined conditions for human iPSC derivation and culture.Nat Methods 8:424–429）。

Gibco Essential 8培养基中bFGF的作用是什么？

bFGF对于细胞的存活与增殖至关重要。

Gibco RevitaCell添加剂适用于任何种类的PSC生长培养基么？

我们测试和确认了适用于下列PSC生长培养基：Gibco Essential 8培养基，Gibco StemPro hESC SFM，mTesR1培养基和含有Gibco KnockOut血清替代物（KSR）的饲养层依赖的培养基。

源自皮肤活检样本的成纤维细胞能否培养于Gibco Essential 8培养基中，以实现无异源成份的培养条件？

可以，源自皮肤活检样本的成纤维细胞可在添加有EGF，凝血酶和皮质醇的Gibco Essential 8培养基中培养和增殖（Chen G, Gulbranson DR, Hou Z et al (2010) Chemically defined conditions for human iPSC derivation and culture.Nat Methods 8:424–429.）

View all Essential 8™ 培养基 FAQs

引用和文献 (5)

引用和文献
Abstract
A comprehensive protocol for efficient differentiation of human NPCs into electrically competent neurons.
Authors:Romito E,Battistella I,Plakhova V,Paplekaj A,Forastieri C,Toffolo E,Musio C,Conti L,Battaglioli E,Rusconi F
Journal:Journal of neuroscience methods
PubMed ID:39053772
Inhibition of SARS-CoV-2 Infections in Engineered Human Tissues Using Clinical-Grade Soluble Human ACE2.
Authors:Monteil V, Kwon H, Prado P, Hagelkrüys A, Wimmer RA, Stahl M, Leopoldi A, Garreta E, Hurtado Del Pozo C, Prosper F, Romero JP, Wirnsberger G, Zhang H, Slutsky AS, Conder R, Montserrat N, Mirazimi A, Penninger JM
Journal:Cell
PubMed ID:32333836
'We have previously provided the first genetic evidence that angiotensin converting enzyme 2 (ACE2) is the critical receptor for severe acute respiratory syndrome coronavirus (SARS-CoV), and ACE2 protects the lung from injury, providing a molecular explanation for the severe lung failure and death due to SARS-CoV infections. ACE2 has now ... More
Scalable topographies to support proliferation and Oct4 expression by human induced pluripotent stem cells.
Authors:Reimer A, Vasilevich A, Hulshof F, Viswanathan P, van Blitterswijk CA, de Boer J, Watt FM,
Journal:Sci Rep
PubMed ID:26757610
It is well established that topographical features modulate cell behaviour, including cell morphology, proliferation and differentiation. To define the effects of topography on human induced pluripotent stem cells (iPSC), we plated cells on a topographical library containing over 1000 different features in medium lacking animal products (xeno-free). Using high content ... More
Defined Essential 8™ Medium and Vitronectin Efficiently Support Scalable Xeno-Free Expansion of Human Induced Pluripotent Stem Cells in Stirred Microcarrier Culture Systems.
Authors:Badenes SM, Fernandes TG, Cordeiro CS, Boucher S, Kuninger D, Vemuri MC, Diogo MM, Cabral JM,
Journal:PLoS One
PubMed ID:26999816
Human induced pluripotent stem (hiPS) cell culture using Essential 8™ xeno-free medium and the defined xeno-free matrix vitronectin was successfully implemented under adherent conditions. This matrix was able to support hiPS cell expansion either in coated plates or on polystyrene-coated microcarriers, while maintaining hiPS cell functionality and pluripotency. Importantly, scale-up ... More
A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids.
Authors:Yang L, Han Y, Nilsson-Payant BE, Gupta V, Wang P, Duan X, Tang X, Zhu J, Zhao Z, Jaffré F, Zhang T, Kim TW, Harschnitz O, Redmond D, Houghton S, Liu C, Naji A, Ciceri G, Guttikonda S, Bram Y, Nguyen DT, Cioffi M, Chandar V, Hoagland DA, Huang Y, Xiang J, Wang H, Lyden D, Borczuk A, Chen HJ, Studer L, Pan FC, Ho DD, tenOever BR, Evans T, Schwartz RE, Chen S
Journal:Cell Stem Cell
PubMed ID:32579880
SARS-CoV-2 has caused the COVID-19 pandemic. There is an urgent need for physiological models to study SARS-CoV-2 infection using human disease-relevant cells. COVID-19 pathophysiology includes respiratory failure but involves other organ systems including gut, liver, heart, and pancreas. We present an experimental platform comprised of cell and organoid derivatives from ... More