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Invitrogen
ATPIF1 Monoclonal Antibody (5E2D7)
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FIGURE: 1 / 26
ATPIF1 Antibody (A-21355) in ICC/IF
CAKI cells were plated on coverslips overnight. The next day cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (Product # R37602) according to protocol. Following suppression of background binding using Image-iT® FX Signal enhancer (Product # R37107), cells were incubated with 3 µg/mL anti-ATP synthase subunit IF1 (Product # A-21355) for labeling of mitochondria for 30 min at room temperature and washed three times with dPBS, followed by Alexa Fluor® 594 goat anti-rabbit IgG antibody ReadyProbes® reagent ... View More
Product Details
A-21355
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Published species
Bovine,
C. elegans,
Dog,
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
5E2D7
Immunogen
Recombinant IF1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
purified
Storage buffer
PBS, pH 7.4
Contains
0.02% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody was produced in vitro using hybridomas grown in serum-free medium, then purified by biochemical fractionation, and is near homogeneity as judged by SDS-PAGE.
This antibody recognizes bands at ~10 and ~18 kDa on western analysis.
Target Information
Complex V, also called F1F0 ATPase or ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP + phosphate to ATP, in oxidative phosphorylation, and as a proton pumping ATPase. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The enzyme was thought to be localized exclusively to mitochondria; however, it has recently been identified on the plasma membrane of several cell types including hepatocytes where it functions as the HDL receptor, on endothelial cells where it may act as the angiostatin receptor, and on the surface of cancer cells. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits; while the F0 consists of a, b, and c subunits. The enzyme in mammals is composed of 17 subunits, five of which make up the easily detached F1. The remainder subunits are components of two stalk domains and the proton pumping F0 part of the machinery. Two of the subunits of the F0 part are encoded on mitochondrial DNA while the other subunits are nuclearly encoded. Mutations in the mitochondrial-encoded subunits of ATP synthase (Complex V) cause OXPHOS disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATP synthase F1 subunit epsilon; ATP synthase inhibitor protein; ATPase inhibitor (rat mitochondrial IF1 protein); ATPase inhibitor protein; ATPase inhibitor, mitochondrial; ATPase inhibitory factor 1; IF(1); IF1; Inhibitor of F(1)F(o)-ATPase
Gene Aliases: ATP5IF1; ATPI; ATPIF1; ATPIP; IF(1); If1; IF1PA; IP
UniProt ID: (Bovine) P01096, (Human) Q5JXL8, (Mouse) O35143, (Rat) Q03344
Entrez Gene ID: (Bovine) 327699, (Human) 93974, (Mouse) 11983, (Rat) 25392
negative regulation of endothelial cell proliferation
heme biosynthetic process
erythrocyte differentiation
protein homotetramerization
negative regulation of hydrolase activity
angiogenesis
generation of precursor metabolites and energy
folic acid-containing compound biosynthetic process
negative regulation of ATPase activity
tetrahydrofolate interconversion
protein homooligomerization
mitochondrial depolarization
reactive oxygen species metabolic process
mitophagy in response to mitochondrial depolarization
positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
positive regulation of proteolysis involved in cellular protein catabolic process
regulation of protein targeting to mitochondrion
regulation of ATP metabolic process
regulation of gene expression
negative regulation of nucleotide metabolic process
regulation of mitophagy
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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