A hyperfused mitochondrial state achieved at G1-S regulates cyclin E buildup and entry into S phase.
AuthorsMitra K, Wunder C, Roysam B, Lin G, Lippincott-Schwartz J,
JournalProc Natl Acad Sci U S A
PubMed ID19617534
Mitochondria undergo fission-fusion events that render these organelles highly dynamic in cells. We report a relationship between mitochondrial form and cell cycle control at the G(1)-S boundary. Mitochondria convert from isolated, fragmented elements into a hyperfused, giant network at G(1)-S transition. The network is electrically continuous and has greater ATP ... More
Cytoplasmic dynein/dynactin drives kinetochore protein transport to the spindle poles and has a role in mitotic spindle checkpoint inactivation.
'We discovered that many proteins located in the kinetochore outer domain, but not the inner core, are depleted from kinetochores and accumulate at spindle poles when ATP production is suppressed in PtK1 cells, and that microtubule depolymerization inhibits this process. These proteins include the microtubule motors CENP-E and cytoplasmic dynein, ... More
Comparison of MTT and ATP-based assays for the measurement of viable cell number.
AuthorsPetty RD, Sutherland LA, Hunter EM, Cree IA
JournalJ Biolumin Chemilumin
PubMed ID7762413
'Cell viability assays are widely used to assess the effect chemotherapeutic drugs and other agents on cell lines and have shown promise for the prediction of tumour chemosensitivity. In this study we have compared two viability assays using Daudi and CCRF-CEM cell lines over a range of 1500-100,000 cells/well of ... More
Calorie restriction induces mitochondrial biogenesis and bioenergetic efficiency.
AuthorsLópez-Lluch G, Hunt N, Jones B, Zhu M, Jamieson H, Hilmer S, Cascajo MV, Allard J, Ingram DK, Navas P, de Cabo R
JournalProc Natl Acad Sci U S A
PubMed ID16446459
'Age-related accumulation of cellular damage and death has been linked to oxidative stress. Calorie restriction (CR) is the most robust, nongenetic intervention that increases lifespan and reduces the rate of aging in a variety of species. Mechanisms responsible for the antiaging effects of CR remain uncertain, but reduction of oxidative ... More
Dynamics of bacteriophage T4 presynaptic filament assembly from extrinsic fluorescence measurements of Gp32-single-stranded DNA interactions.
AuthorsLiu J, Qian N, Morrical SW
JournalJ Biol Chem
PubMed ID16829679
'In the bacteriophage T4 homologous recombination system, presynaptic filament assembly on single-stranded (ssDNA) DNA requires UvsX recombinase, UvsY mediator, and Gp32 ssDNA-binding proteins. Gp32 exerts both positive and negative effects on filament assembly: positive by denaturing ssDNA secondary structure, and negative by competing with UvsX for ssDNA binding sites. UvsY ... More
Method for measuring ATP production in isolated mitochondria: ATP production in brain and liver mitochondria of Fischer-344 rats with age and caloric restriction.
AuthorsDrew B, Leeuwenburgh C
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID12855419
'The production of ATP is vital for muscle contraction, chemiosmotic homeostasis, and normal cellular function. Many studies have measured ATP content or qualitative changes in ATP production, but few have quantified ATP production in vivo in isolated mitochondria. Because of the importance of understanding the energy capacity of mitochondria in ... More
Effects of aging and caloric restriction on mitochondrial energy production in gastrocnemius muscle and heart.
AuthorsDrew B, Phaneuf S, Dirks A, Selman C, Gredilla R, Lezza A, Barja G, Leeuwenburgh C
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID12388443
'Mitochondria are chronically exposed to reactive oxygen intermediates. As a result, various tissues, including skeletal muscle and heart, are characterized by an age-associated increase in reactive oxidant-induced mitochondrial DNA (mtDNA) damage. It has been postulated that these alterations may result in a decline in the content and rate of production ... More
Changes in cytosolic Ca2+ levels regulate Bcl-xS and Bcl-xL expression in spermatogenic cells during apoptotic death.
AuthorsMishra DP, Pal R, Shaha C
JournalJ Biol Chem
PubMed ID16301318
'Bcl-x exists in two isoforms, the anti-apoptotic form Bcl-xL and the proapoptotic form Bcl-xS. The critical balance between the two forms appears to be important for cell survival; however, it is still not clear exactly how the vital balance is maintained. Using an in vitro spermatogenic cell apoptosis model, this ... More
Guanylyl cyclase is an ATP sensor coupling nitric oxide signaling to cell metabolism.
AuthorsRuiz-Stewart I, Tiyyagura SR, Lin JE, Kazerounian S, Pitari GM, Schulz S, Martin E, Murad F, Waldman SA
JournalProc Natl Acad Sci U S A
PubMed ID14684830
'Defending cellular integrity against disturbances in intracellular concentrations of ATP ([ATP](i)) is predicated on coordinating the selection of substrates and their flux through metabolic pathways (metabolic signaling), ATP transfer from sites of production to utilization (energetic signaling), and the regulation of processes consuming energy (cell signaling). Whereas NO and its ... More
Activation of NLRP3 and AIM2 inflammasomes by Porphyromonas gingivalis infection.
AuthorsPark E, Na HS, Song YR, Shin SY, Kim YM, Chung J,
Journal
PubMed ID24126516
'Porphyromonas gingivalis, a major periodontopathogen, is involved in the pathogenesis of periodontitis. Interleukin-1ß (IL-1ß), a proinflammatory cytokine, regulates innate immune responses and is critical for the host defense against bacterial infection. However, excessive IL-1ß is linked to periodontal destruction. IL-1ß synthesis, maturation, and secretion are tightly regulated by Toll-like receptor ... More
FOXO1 promotes wound healing through the up-regulation of TGF-ß1 and prevention of oxidative stress.
'Keratinocyte mobilization is a critical aspect of wound re-epithelialization, but the mechanisms that control its precise regulation remain poorly understood. We set out to test the hypothesis that forkhead box O1 (FOXO1) has a negative effect on healing because of its capacity to inhibit proliferation and promote apoptosis. Contrary to ... More
Glial cell inhibition of neurons by release of ATP.
AuthorsNewman EA
JournalJ Neurosci
PubMed ID12629170
'ATP is released by neurons and functions as a neurotransmitter and modulator in the CNS. Here I show that ATP released from glial cells can also serve as a potent neuromodulator, inhibiting neurons in the retina of the rat. Activation of glial cells by focal ejection of ATP, ATPgammaS, dopamine, ... More
ATP synthase. Conditions under which all catalytic sites of the F1 moiety are kinetically equivalent in hydrolyzing ATP.
AuthorsReynafarje BD, Pedersen PL
JournalJ Biol Chem
PubMed ID8955079
'Conditions have been reported under which the F1 moiety of bovine heart ATP synthase catalyzes the hydrolysis of ATP by an apparently cooperative mechanism in which the slow rate of hydrolysis at a single catalytic site (unisite catalysis) is enhanced more than 10(6)-fold when ATP is added in excess to ... More
The mammalian longevity-associated gene product p66shc regulates mitochondrial metabolism.
AuthorsNemoto S, Combs CA, French S, Ahn BH, Fergusson MM, Balaban RS, Finkel T
JournalJ Biol Chem
PubMed ID16481327
'Previous studies have determined that mice with a homozygous deletion in the adapter protein p66(shc) have an extended life span and that cells derived from these mice exhibit lower levels of reactive oxygen species. Here we demonstrate that a fraction of p66(shc) localizes to the mitochondria and that p66(shc-/-) fibroblasts ... More
The use of ATP bioluminescence as a measure of cell proliferation and cytotoxicity.
'Adenosine triphosphate (ATP) bioluminescence was used to determine whether there was a linear relationship between cultured cell number and measured luminescence using the luciferin-luciferase reaction. In all the cells tested including peripheral blood mononuclear cells (MNC), MOLT-4, HL-60, TF-1, NFS-60 and L-929 cell lines there was a significant correlation as ... More
SIR-2.1 integrates metabolic homeostasis with the reproductive neuromuscular excitability in early aging male Caenorhabditis elegans.
AuthorsGuo X, García LR,
Journal
PubMed ID24755287
'The decline of aging C. elegans male''s mating behavior is correlated with the increased excitability of the cholinergic circuitry that executes copulation. In this study, we show that the mating circuits'' functional durability depends on the metabolic regulator SIR-2.1, a NAD(+)-dependent histone deacetylase. Aging sir-2.1(0) males display accelerated mating behavior ... More
CD8 memory T cells have a bioenergetic advantage that underlies their rapid recall ability.
Authorsvan der Windt GJ, O'Sullivan D, Everts B, Huang SC, Buck MD, Curtis JD, Chang CH, Smith AM, Ai T, Faubert B, Jones RG, Pearce EJ, Pearce EL,
Journal
PubMed ID23940348
'A characteristic of memory T (TM) cells is their ability to mount faster and stronger responses to reinfection than naïve T (TN) cells do in response to an initial infection. However, the mechanisms that allow this rapid recall are not completely understood. We found that CD8 TM cells have more ... More
Energy metabolism regulates clathrin adaptors at the trans-Golgi network and endosomes.
AuthorsAoh QL, Hung CW, Duncan MC,
JournalMol Biol Cell
PubMed ID23345590
'Glucose is a master regulator of cell behavior in the yeast Saccharomyces cerevisiae. It acts as both a metabolic substrate and a potent regulator of intracellular signaling cascades. Glucose starvation induces the transient delocalization and then partial relocalization of clathrin adaptors at the trans-Golgi network and endosomes. Although these localization ... More
Reliable luminescent detection of subpicomole amounts of ATP using a scintillation counter.
AuthorsPhillippy BQ
JournalBiotechniques
PubMed ID8024775
Determination of myocardial high-energy phosphates using bioluminescence.
AuthorsEllis RJ, Gardner C
JournalAnal Biochem
PubMed ID7457841
Use of the liquid scintillation spectrometer for determining adenosine triphosphate by the luciferase enzyme.
AuthorsStanley PE, Williams SG
JournalAnal Biochem
PubMed ID4307203
Rapid detection of bacterial growth in blood cultures by bioluminescent assay of bacterial ATP.
AuthorsMolin O, Nilsson L, Anséhn S
JournalJ Clin Microbiol
PubMed ID6630442
A method for rapid detection of bacterial growth in blood cultures by bioluminescent assay of bacterial ATP was developed. Samples from blood cultures were treated with a blood-lysing detergent combined with an ATP-hydrolyzing enzyme to destroy blood cell ATP. Blood cell ATP which was bound to cell debris and escaped ... More
Extracellular electron transport-mediated Fe(III) reduction by a community of alkaliphilic bacteria that use flavins as electron shuttles.
AuthorsFuller SJ, McMillan DG, Renz MB, Schmidt M, Burke IT, Stewart DI,
Journal
PubMed ID24141133
The biochemical and molecular mechanisms used by alkaliphilic bacterial communities to reduce metals in the environment are currently unknown. We demonstrate that an alkaliphilic (pH > 9) consortium dominated by Tissierella, Clostridium, and Alkaliphilus spp. is capable of using iron (Fe(3+)) as a final electron acceptor under anaerobic conditions. Iron ... More
AuthorsGulshan K, Brubaker G, Wang S, Hazen SL, Smith JD,
Journal
PubMed ID24220029
The phosphatidylserine (PS) floppase activity (outward translocation) of ABCA1 leads to plasma membrane remodeling that plays a role in lipid efflux to apolipoprotein A-I (apoAI) generating nascent high density lipoprotein. The Tangier disease W590S ABCA1 mutation has defective PS floppase activity and diminished cholesterol efflux activity. Here, we report that ... More
Miro-1 links mitochondria and microtubule Dynein motors to control lymphocyte migration and polarity.
The recruitment of leukocytes to sites of inflammation is crucial for a functional immune response. In the present work, we explored the role of mitochondria in lymphocyte adhesion, polarity, and migration. We show that during adhesion to the activated endothelium under physiological flow conditions, lymphocyte mitochondria redistribute to the adhesion ... More
Cysteine catabolism: a novel metabolic pathway contributing to glioblastoma growth.
AuthorsPrabhu A, Sarcar B, Kahali S, Yuan Z, Johnson JJ, Adam KP, Kensicki E, Chinnaiyan P,
Journal
PubMed ID24351290
The relevance of cysteine metabolism in cancer has gained considerable interest in recent years, largely focusing on its role in generating the antioxidant glutathione. Through metabolomic profiling using a combination of high-throughput liquid and gas chromatography-based mass spectrometry on a total of 69 patient-derived glioma specimens, this report documents the ... More
Acute inhibition of excessive mitochondrial fission after myocardial infarction prevents long-term cardiac dysfunction.
Ischemia and reperfusion (IR) injury remains a major cause of morbidity and mortality and multiple molecular and cellular pathways have been implicated in this injury. We determined whether acute inhibition of excessive mitochondrial fission at the onset of reperfusion improves mitochondrial dysfunction and cardiac contractility postmyocardial infarction in rats. We ... More
Rapamycin drives selection against a pathogenic heteroplasmic mitochondrial DNA mutation.
AuthorsDai Y, Zheng K, Clark J, Swerdlow RH, Pulst SM, Sutton JP, Shinobu LA, Simon DK,
Journal
PubMed ID24101601
Mitochondrial DNA (mtDNA) mutations cause a variety of mitochondrial disorders for which effective treatments are lacking. Emerging data indicate that selective mitochondrial degradation through autophagy (mitophagy) plays a critical role in mitochondrial quality control. Inhibition of mammalian target of rapamycin (mTOR) kinase activity can activate mitophagy. To test the hypothesis ... More
Globin-coupled heme containing oxygen sensor soluble adenylate cyclase in Leishmania prevents cell death during hypoxia.
AuthorsSen Santara S, Roy J, Mukherjee S, Bose M, Saha R, Adak S,
Journal
PubMed ID24082109
Globin and adenylate cyclase play individually numerous crucial roles in eukaryotic organisms. Comparison of the amino acid sequences of globins and adenylate cyclase from prokaryotic to eukaryotic organisms suggests that they share an early common ancestor, even though these proteins execute different functions in these two kingdoms. The latest studies ... More
Functional characterization of SbmA, a bacterial inner membrane transporter required for importing the antimicrobial peptide Bac7(1-35).
AuthorsRunti G, Lopez Ruiz Mdel C, Stoilova T, Hussain R, Jennions M, Choudhury HG, Benincasa M, Gennaro R, Beis K, Scocchi M,
Journal
PubMed ID24078610
SbmA is an inner membrane protein of Gram-negative bacteria that is involved in the internalization of glycopeptides and prokaryotic and eukaryotic antimicrobial peptides, as well as of peptide nucleic acid (PNA) oligomers. The SbmA homolog BacA is required for the development of Sinorhizobium meliloti bacteroids within plant cells and favors ... More
Invadolysin, a conserved lipid-droplet-associated metalloproteinase, is required for mitochondrial function in Drosophila.
AuthorsDi Cara F, Duca E, Dunbar DR, Cagney G, Heck MM,
Journal
PubMed ID23943867
Mitochondria are the main producers of ATP, the principal energy source of the cell, and reactive oxygen species (ROS), important signaling molecules. Mitochondrial morphogenesis and function depend on a hierarchical network of mechanisms in which proteases appear to be center stage. The invadolysin gene encodes an essential conserved metalloproteinase of ... More
Single molecule imaging reveals a major role for diffusion in the exploration of ciliary space by signaling receptors.
The dynamic organization of signaling cascades inside primary cilia is key to signal propagation. Yet little is known about the dynamics of ciliary membrane proteins besides a possible role for motor-driven Intraflagellar Transport (IFT). To characterize these dynamics, we imaged single molecules of Somatostatin Receptor 3 (SSTR3, a GPCR) and ... More
Defective extracellular pyrophosphate metabolism promotes vascular calcification in a mouse model of Hutchinson-Gilford progeria syndrome that is ameliorated on pyrophosphate treatment.
Progerin is a mutant form of lamin A responsible for Hutchinson-Gilford progeria syndrome (HGPS), a premature aging disorder characterized by excessive atherosclerosis and vascular calcification that leads to premature death, predominantly of myocardial infarction or stroke. The goal of this study was to investigate mechanisms that cause excessive vascular calcification ... More
Interkingdom signaling induces Streptococcus pneumoniae biofilm dispersion and transition from asymptomatic colonization to disease.
Streptococcus pneumoniae is a common human nasopharyngeal commensal colonizing 10% to 40% of healthy individuals, depending on age. Despite a low invasive disease rate, widespread carriage ensures that infection occurs often enough to make S. pneumoniae a leading bacterial cause of respiratory disease worldwide. However, the mechanisms behind transition from ... More
Human prostasomes express glycolytic enzymes with capacity for ATP production.
AuthorsRonquist KG, Ek B, Stavreus-Evers A, Larsson A, Ronquist G,
JournalAm J Physiol Endocrinol Metab
PubMed ID23341497
Prostasomes are prostate-derived, exosome-like microvesicles that transmit signaling complexes between the acinar epithelial cells of the prostate and sperm cells. The vast majority of prostasomes have a diameter of 30-200 nm, and they are generally surrounded by a classical membrane bilayer. Using a selected proteomic approach, it became increasingly clear ... More
Coordinated elevation of mitochondrial oxidative phosphorylation and autophagy help drive hepatocyte polarization.
Cell polarization requires increased cellular energy and metabolic output, but how these energetic demands are met by polarizing cells is unclear. To address these issues, we investigated the roles of mitochondrial bioenergetics and autophagy during cell polarization of hepatocytes cultured in a collagen sandwich system. We found that as the ... More
CyclinB1/Cdk1 phosphorylates mitochondrial antioxidant MnSOD in cell adaptive response to radiation stress.
AuthorsCandas D, Fan M, Nantajit D, Vaughan AT, Murley JS, Woloschak GE, Grdina DJ, Li JJ,
JournalJ Mol Cell Biol
PubMed ID23243068
Manganese superoxide dismutase (MnSOD), a major antioxidant enzyme within the mitochondria, is responsible for the detoxification of free radicals generated by cellular metabolism and environmental/therapeutic irradiation. Cell cycle-dependent kinase Cdk1, along with its regulatory partner CyclinB1, plays important roles in the regulation of cell cycle progression as well as in ... More
Antioxidant enzymes mediate survival of breast cancer cells deprived of extracellular matrix.
AuthorsDavison CA, Durbin SM, Thau MR, Zellmer VR, Chapman SE, Diener J, Wathen C, Leevy WM, Schafer ZT,
Journal
PubMed ID23771908
Metastasis by cancer cells relies upon the acquisition of the ability to evade anoikis, a cell death process elicited by detachment from extracellular matrix (ECM). The molecular mechanisms that ECM-detached cancer cells use to survive are not understood. Striking increases in reactive oxygen species (ROS) occur in ECM-detached mammary epithelial ... More
SLP-2 is required for stress-induced mitochondrial hyperfusion.
AuthorsTondera D, Grandemange S, Jourdain A, Karbowski M, Mattenberger Y, Herzig S, Da Cruz S, Clerc P, Raschke I, Merkwirth C, Ehses S, Krause F, Chan DC, Alexander C, Bauer C, Youle R, Langer T, Martinou JC,
JournalEMBO J
PubMed ID19360003
Mitochondria are dynamic organelles, the morphology of which results from an equilibrium between two opposing processes, fusion and fission. Mitochondrial fusion relies on dynamin-related GTPases, the mitofusins (MFN1 and 2) in the outer mitochondrial membrane and OPA1 (optic atrophy 1) in the inner mitochondrial membrane. Apart from a role in ... More
A cell-based high-throughput assay system reveals modulation of oxidative and nonoxidative glucose metabolism due to commonly used organic solvents.
AuthorsZimmermann S, Zarse K, Schulz TJ, Siems K, Müller-Kuhrt L, Birringer M, Ristow M,
JournalHorm Metab Res
PubMed ID18197582
A 96-well format screening system was generated to quantify changes in nonoxidative glucose metabolism and oxidative pyruvate metabolism. D-Glucose uptake from the supernatant media was quantified by the glucose oxidase method, and L-lactate production of cells was quantified by the lactate dehydrogenase method applied on supernatant media. Mitochondrial membrane potential ... More
JNK1/2 regulates ER-mitochondrial Ca2+ cross-talk during IL-1ß-mediated cell death in RINm5F and human primary ß-cells.
AuthorsVerma G, Bhatia H, Datta M,
JournalMol Biol Cell
PubMed ID23615449
Elevated interleukin-1ß (IL-1ß) induces apoptosis in pancreatic ß-cells through endoplasmic reticulum (ER) stress induction and subsequent c-jun-N-terminal kinase 1/2 (JNK1/2) activation. In earlier work we showed that JNK1/2 activation is initiated before ER stress and apoptotic induction in response to IL-1ß. However, the detailed regulatory mechanisms are not completely understood. ... More
Amyloid-beta overproduction causes abnormal mitochondrial dynamics via differential modulation of mitochondrial fission/fusion proteins.
AuthorsWang X, Su B, Siedlak SL, Moreira PI, Fujioka H, Wang Y, Casadesus G, Zhu X,
JournalProc Natl Acad Sci U S A
PubMed ID19050078
Mitochondrial dysfunction is a prominent feature of Alzheimer disease but the underlying mechanism is unclear. In this study, we investigated the effect of amyloid precursor protein (APP) and amyloid beta on mitochondrial dynamics in neurons. Confocal and electron microscopic analysis demonstrated that approximately 40% M17 cells overexpressing WT APP (APPwt ... More
Estrogen Receptor {beta} as a Mitochondrial Vulnerability Factor.
AuthorsYang SH, Sarkar SN, Liu R, Perez EJ, Wang X, Wen Y, Yan LJ, Simpkins JW,
JournalJ Biol Chem
PubMed ID19189968
We recently demonstrated mitochondrial localization of estrogen receptor beta (ERbeta). We herein confirm the mitochondrial localization of ERbeta by the loss of mitochondrial ERbeta immunoreactivity in ERbeta knockdown cells. A phenotype change characterized as an increase in resistance to oxidative stressors is associated with ERbeta knockdown. ERbeta knockdown results in ... More
Bioluminometric method for real-time detection of ATPase activity.
AuthorsKaramohamed S, Guidotti G
JournalBiotechniques
PubMed ID11515379
We have developed a real-time, simple, and sensitive method for the detection of ATP hydrolysis activity (ATPase) of apyrase (EC 3.6.1.5). The assay is based on the continuous monitoring of the ATP hydrolysis reaction using the firefly luciferase system. The method is sensitive and yields linear responses between 0.7 and ... More
Mitochondria in eosinophils: functional role in apoptosis but not respiration.
AuthorsPeachman KK, Lyles DS, Bass DA
JournalProc Natl Acad Sci U S A
PubMed ID11172017
In most eukaryotic cells, mitochondria use the respiratory chain to produce a proton gradient, which is then harnessed for the synthesis of ATP. Recently, mitochondrial roles in regulation of apoptosis have been discovered in many cell types. Eosinophils (Eos) die by apoptosis, but the presence and function of mitochondria in ... More
An enzymatic cycling method using pyruvate orthophosphate dikinase and firefly luciferase for the simultaneous determination of ATP and AMP (RNA).
AuthorsSakakibara T, Murakami S, Eisaki N, Nakajima M, Imai K
JournalAnal Biochem
PubMed ID10036167
A novel bioluminescent enzymatic cycling assay for ATP and AMP with concomitant use of firefly luciferase and pyruvate orthophosphate dikinase (PPDK) was developed. In this system, AMP and pyrophosphate produced from ATP by firefly luciferase were converted back into ATP by PPDK. This resulted in constant luminescence once the stable ... More
ATP assay: ability to distinguish cytostatic from cytocidal anticancer drug effects.
AuthorsGarewal HS, Ahmann FR, Schifman RB, Celniker A
JournalJ Natl Cancer Inst
PubMed ID3464795
A bioluminescence assay for ATP was adapted to human cancer cell lines and used to study the effect of anticancer drugs on malignant cell growth by following serial ATP measurements. Eleven drugs were tested against a colon cancer cell line (WiDR). Excellent correlation was observed between simultaneously performed soft-agar colony-forming ... More
AuthorsZha X, Pierini LM, Leopold PL, Skiba PJ, Tabas I, Maxfield FR
JournalJ Cell Biol
PubMed ID9425152
ATP hydrolysis has been regarded as a general requirement for internalization processes in mammalian cells. We found, however, that treatment of ATP-depleted macrophages and fibroblasts with exogenous sphingomyelinase (SMase) rapidly induces formation of numerous vesicles that pinch off from the plasma membrane; the process is complete within 10 min after ... More
Control of mitochondrial redox balance and cellular defense against oxidative damage by mitochondrial NADP+-dependent isocitrate dehydrogenase.
AuthorsJo SH, Son MK, Koh HJ, Lee SM, Song IH, Kim YO, Lee YS, Jeong KS, Kim WB, Park JW, Song BJ, Huh TL, Huhe TL
JournalJ Biol Chem
PubMed ID11278619
Mitochondria are the major organelles that produce reactive oxygen species (ROS) and the main target of ROS-induced damage as observed in various pathological states including aging. Production of NADPH required for the regeneration of glutathione in the mitochondria is critical for scavenging mitochondrial ROS through glutathione reductase and peroxidase systems. ... More
ATP mediates calcium signaling between astrocytes and microglial cells: modulation by IFN-gamma.
AuthorsVerderio C, Matteoli M
JournalJ Immunol
PubMed ID11342663
Calcium-mediated intercellular communication is a mechanism by which astrocytes communicate with each other and modulate the activity of adjacent cells, including neurons and oligodendrocytes. We have investigated whether microglia, the immune effector cells involved in several diseases of the CNS, are actively involved in this communication network. To address this ... More
ATP concentrations in multicellular tumor spheroids assessed by single photon imaging and quantitative bioluminescence.
AuthorsWalenta S, Dötsch J, Mueller-Klieser W
JournalEur J Cell Biol
PubMed ID2081538
To evaluate the interrelationship among the cellular energy status and the development of necrosis in tumor microregions, local ATP concentrations and the extent of necrosis were determined in multicellular tumor spheroids, i.e., in spherical tumor cell aggregates. The spheroids were grown in rotated suspension cultures using EMT6 cells that were ... More
A sperm-specific Na+/H+ exchanger (sNHE) is critical for expression and in vivo bicarbonate regulation of the soluble adenylyl cyclase (sAC).
AuthorsWang D, Hu J, Bobulescu IA, Quill TA, McLeroy P, Moe OW, Garbers DL
JournalProc Natl Acad Sci U S A
PubMed ID17517652
We previously identified a sperm-specific Na(+)/H(+) exchanger (sNHE) principally localized to the flagellum. Disruption of the sNHE gene in mice resulted in absolute male infertility associated with a complete loss of sperm motility. Here, we show that the sNHE-null spermatozoa fail to develop the cAMP-dependent protein tyrosine phosphorylation that coincides ... More
Bacteriuria screening by direct bioluminescence assay of ATP.
AuthorsSchifman RB, Wieden M, Brooker J, Chery M, Delduca M, Norgard K, Palen C, Reis N, Swanson J, White J
JournalJ Clin Microbiol
PubMed ID6490851
A direct bioluminescence assay for bacteriuria screening is described and compared with the MS-2 system (Abbott Laboratories, Irvine, Tex.) and the chemical strip, Gram smear, and calibrated-loop methods. A total of 973 specimens were tested. Unlike previously described bioluminescence methods, this test measures total ATP in urine without pretreatment of ... More
A convenient one-step extraction of cellular ATP using boiling water for the luciferin-luciferase assay of ATP.
AuthorsYang NC, Ho WM, Chen YH, Hu ML
JournalAnal Biochem
PubMed ID12123672
Cellular ATP is commonly determined as production of bioluminescence using a luciferin-luciferase reaction system. Before the measurement of bioluminescence, cellular ATP must first be extracted. Two commonly used extraction methods are: () Tris-borate buffer (pH 9.2) coupled with a heating process (to inactivate ATPase) and () perchloric acid followed by ... More
Role of mitochondrial Na+ concentration, measured by CoroNa red, in the protection of metabolically inhibited MDCK cells.
AuthorsBaron S, Caplanusi A, van de Ven M, Radu M, Despa S, Lambrichts I, Ameloot M, Steels P, Smets I
JournalJ Am Soc Nephrol
PubMed ID16221870
In ischemic or hypoxic tissues, elevated cytosolic calcium levels can induce lethal processes. Mitochondria, besides the endoplasmic reticulum, play a key role in clearing excessive cytosolic Ca2+. In a previous study, it was suggested that the clearance of cytosolic Ca2+, after approximately 18 min of metabolic inhibition (MI) in renal ... More
Mechanistic and functional studies of the interaction of a proline-rich antimicrobial peptide with mammalian cells.
AuthorsTomasinsig L, Skerlavaj B, Papo N, Giabbai B, Shai Y, Zanetti M
JournalJ Biol Chem
PubMed ID16257969
Mammalian antimicrobial peptides provide rapid defense against infection by inactivating pathogens and by influencing the functions of cells involved in defense responses. Although the direct antibacterial properties of these peptides have been widely characterized, their multiple effects on host cells are only beginning to surface. Here we investigated the mechanistic ... More
Increase in cytosolic Ca2+ levels through the activation of non-selective cation channels induced by oxidative stress causes mitochondrial depolarization leading to apoptosis-like death in Leishmania donovani promastigotes.
AuthorsMukherjee SB, Das M, Sudhandiran G, Shaha C
JournalJ Biol Chem
PubMed ID11983701
Reactive oxygen species are important regulators of protozoal infection. Promastigotes of Leishmania donovani, the causative agent of Kala-azar, undergo an apoptosis-like death upon exposure to H2O2. The present study shows that upon activation of death response by H2O2, a dose- and time-dependent loss of mitochondrial membrane potential occurs. This loss ... More
An Ancient, Unified Mechanism for Metformin Growth Inhibition in C. elegans and Cancer.
Authors
JournalCell
PubMed ID27984722
The Energetics and Physiological Impact of Cohesin Extrusion.
Authors
JournalCell
PubMed ID29706548
Small-Molecule MYC Inhibitors Suppress Tumor Growth and Enhance Immunotherapy.
Authors
JournalCancer Cell
PubMed ID31679823
Proteome Instability Is a Therapeutic Vulnerability in Mismatch Repair-Deficient Cancer.
Authors
JournalCancer Cell
PubMed ID32109374
p113 isoform encoded by CUX1 circular RNA drives tumor progression via facilitating ZRF1/BRD4 transactivation.
Authors
JournalMol Cancer
PubMed ID34579723
Mitochondrial Permeability Uncouples Elevated Autophagy and Lifespan Extension.
Authors
JournalCell
PubMed ID30929899
Muscle mitohormesis promotes longevity via systemic repression of insulin signaling.
Authors
JournalCell
PubMed ID24243023
Structure and Function of a Bacterial Gap Junction Analog.
Authors
JournalCell
PubMed ID31299201
Retinal lipid and glucose metabolism dictates angiogenesis through the lipid sensor Ffar1.
Authors
JournalNat Med
PubMed ID26974308
Glycolytic ATP fuels phosphoinositide 3-kinase signaling to support effector T helper 17 cell responses.