Ion 通用文库定量试剂盒是一种易于使用、灵活的解决方案,可准确定量 Ion 16STM 宏基因组试剂盒生成的文库起始量,用于 Ion Torrent™ 半导体测序。本试剂盒提供定量分析飞摩尔水平含量的了解更多信息
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货号
数量
A26217
250 Reactions
货号 A26217
价格(CNY)
-
数量:
250 Reactions
Ion 通用文库定量试剂盒是一种易于使用、灵活的解决方案,可准确定量 Ion 16STM 宏基因组试剂盒生成的文库起始量,用于 Ion Torrent™ 半导体测序。本试剂盒提供定量分析飞摩尔水平含量的 Ion 片段文库所需的 TaqMan™ 分析、TaqMan™ 通用预混液和文库标准品。使用能够快速和标准循环的循环条件,Ion 通用文库定量试剂盒可支持大多数实时平台。与基于非 PCR 或非探针的方法相比,这种实时荧光定量 PCR 试剂盒可实现更高的准确性和特异性,并使 Ion PGM™ 系统测序工作流程使简便快速。
If I accidentally use the Ion Library TaqMan Quantitation Kit instead of the Ion Universal Library Quantitation Kit with my Ion 16S Metagenomics libraries, what might happen?
This may result in a lower observed library yield as determined by qPCR.
What are the components of the Ion Universal Library Quantitation Kit (Cat. No. A26217)?
The components are listed below:
- TaqMan Fast Universal PCR Master Mix: 2 tubes (1.54 mL per tube)
- Ion Library TaqMan Quantitation Assay, 20X: 250 µL
- E. coli DH10B Ion Control Library: 2 tubes (25 µL per tube)
How is the Ion Universal Library Quantitation Kit different from the Ion Library TaqMan Quantitation Kit?
The Ion Universal Library Quantitation Kit is compatible with U-containing amplicons generated by the Ion 16S Metagenomics Kit whereas the Ion Library TaqMan Quantitation Kit is not. For most other Ion libraries, use the Ion Library TaqMan Quantitation Kit [exceptions: (1) RNA Seq libraries prepared using the Ion Total RNA-Seq Kit v2 and (2) amplicon libraries prepared following the Ion Amplicon Library Preparation (Fusion Method) User Guide (http://tools.thermofisher.com/content/sfs/manuals/4468326_IonAmpliconLibraryPrep_FusionMethod_UG.pdf), as these libraries have a truncated P1 (trP1) adapter sequence].
What kit should I use to quantitate library input generated by the Ion 16S Metagenomics Kit?
We recommend using the Ion Universal Library Quantitation Kit, Cat. No. A26217. It is compatible with the uracil-containing amplicons generated by the Ion 16S Metagenomics Kit.