TaqMan™ Advanced miRNA cDNA 合成试剂盒
TaqMan™ Advanced miRNA cDNA 合成试剂盒
Applied Biosystems™

TaqMan™ Advanced miRNA cDNA 合成试剂盒

试剂盒使用3'poly-A 拖尾和适配器序列的5'个连接用于逆转录前延伸样本中存在于末端的成熟 miRNA。
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货号数量
A2800750 cDNA synthesis reactions
货号 A28007
价格（CNY)
4,555.00
Each
添加至购物车
数量:
50 cDNA synthesis reactions
价格（CNY)
4,555.00
Each
添加至购物车

Applied Biosystems™ TaqMan™ Advanced miRNA cDNA 合成试剂盒使用通用型逆转录 (RT) 化学试剂盒制备 cDNA 模板、以便与 TaqMan™ Advanced miRNA 测定一起用于检测和定量生物样本中成熟人 miRNA。

TaqMan Advanced miRNA cDNA 合成试剂盒使用的3'个 poly-A 拖尾和适配器序列的5'个连接用于逆转录前延伸样本中存在于末端的成熟 miRNA。通用 RT 引物识别存在于成熟 miRNA 的 5'和3'延伸末端上的通用序列。样本中所有成熟 miRNA 都被逆转录成 cDNA。

为了改善低表达 miRNA 靶标的检测，然后使用通用 miR-Amp 引物和 miR-Amp 预混液扩增 cDNA ，以均匀增加各个靶标 cDNA 的量，并保持相对差异表达水平。与传统预扩增不同，通用 miR-Amp 引物可识别添加至所有成熟 miRNA 的5'和3'末端的通用序列，确保不产生扩增偏倚。

  • 灵敏度：高灵敏度可以定量低检测低表达的 miRNA 靶标，尤其是在血浆、血清和组织等生物流体中
  • 灵活性：通用 RT 化学可为样本中存在的所有 miRNA 生成通用的 cDNA 模板，从而使研究设计具有灵活性
  • 节省样本：仅需 2μL 血浆/血清样本输入，即可生成足够的 cDNA 用于 600 qPCR 反应（20μL）或 1200 qPCR 反应（10μL）
  • 特异性：5'连接步骤允许沿着整个 miRNA 序列中进行单碱基对区分，包括5'末端难以检测的错配
  • 金标准 TaqMan 化学：该试剂盒设计用于 qPCR 的 TaqMan™ Advanced miRNA 测定，利用 Thermo Fisher Scientifics' 行业领先的生物信息学测定设计流程和验证系统，有助于确保高特异性和最小的交叉反应性，即使对于密切相关的 miRNA 系列也是如此。
仅供科研使用。不可用于诊断程序。
规格
适用于（设备）7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System
反应次数50 cDNA synthesis reactions / 600 qPCR reactions
最佳反应温度42°C
产品线TaqMan™
产品类型cDNA 合成试剂盒
数量50 cDNA synthesis reactions
反应形式分离组分
逆转录酶SuperScript™ III
样品类型RNA
运输条件干冰
容量10 μL
检测方法引物-探针
适用于（应用）miRNA 分析
高 GC PCR 扩增效果高
PCR 方法qPCR
反应速度标准
Unit SizeEach
内容与储存
•10 x PolyA 缓冲液
• ATP、10 mM
• Poly A 酶、5 U/μ L
•5 X 连接酶缓冲液
• RNA 连接酶、10 U/μ L
•50 % PEG 8000
•25 X 连接适配器
•10 X RT 酶混合
•20 X 通用 RT 引物
•dNTP 混合物、100 mM
•20 X miR-Amp 预混液
•5 X RT 缓冲液
•2 X miR-Amp 预混液

储存在 -5至 -30° C，储存在2–-8 °C的2 X miR-Amp 预混液除外。

常见问题解答 (FAQ)

Can I purchase any of the components within the TaqMan Advanced miRNA cDNA Synthesis Kit (Cat. No. A28007) separately?

No, none of the single components within the kit are available as stand-alone. They only can be ordered within the complete kit.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

Do you have a modified protocol for the TaqMan MicroRNA Cells-to-CT Kit (Cat. No. 4391848) to make it compatible with the TaqMan Advanced miRNA Assays?

The workflow to use the Cell-to Ct kit with the TaqMan Advanced miRNA Assays is to use 2 µl of the lysate post cell lysis as input into the TaqMan Advanced miRNA cDNA synthesis Kit.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

What is the concentration of the probe and primer in the 20X TaqMan Advanced miRNA Assay (Cat. No. A25576)?

Unfortunately, these concentrations cannot be disclosed. Use the assay at a final concentration of 1x in the total reaction volume.

Find additional tips, troubleshooting help, and resources within our TaqMan Primers and Probes Support Center.

Do you have any suggestion on how to use the TaqMan Advanced miRNA Assay with Fluidigm system?

Our recommendation for this process would be to carry out a cDNA synthesis according to the TaqMan Advanced miRNA protocol, followed by scaling down QPCR reaction in accordance with the Fluidigm protocol.

At what concentration should I add the spike-in control into my sample for the TaqMan Advanced miRNA Assay?

The final concentration of the spike-in control in your test sample should be 1–10 pM.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Applications Support Center.

View all TaqMan™ Advanced miRNA cDNA 合成试剂盒 FAQs