Human fetal retinal pigment epithelial cells induce apoptosis in the T-cell line Jurkat.
AuthorsFarrokh-Siar L, Rezai KA, Semnani RT, Patel SC, Ernest JT, Peterson EJ, Koretzky GA, van Seventer GA
JournalInvest Ophthalmol Vis Sci
PubMed ID10359333
'PURPOSE: To investigate the mechanism(s) involved in human fetal retinal pigment epithelium (HFRPE)-mediated T-cell death. METHODS: Pure HFRPE cells were isolated and cultured. Normal and interferon (IFN)-gamma-activated HFRPE from early and late in vitro passages were incubated with cells from the human T-cell leukemia line Jurkat (Jkt). Cultures were pulsed ... More
High-resolution mapping reveals topologically distinct cellular pools of phosphatidylserine.
AuthorsFairn GD, Schieber NL, Ariotti N, Murphy S, Kuerschner L, Webb RI, Grinstein S, Parton RG,
JournalJ Cell Biol
PubMed ID21788369
'Phosphatidylserine (PS) plays a central role in cell signaling and in the biosynthesis of other lipids. To date, however, the subcellular distribution and transmembrane topology of this crucial phospholipid remain ill-defined. We transfected cells with a GFP-tagged C2 domain of lactadherin to detect by light and electron microscopy PS exposed ... More
Cigarette smoke extract induces oxidative stress and apoptosis in human lung fibroblasts.
AuthorsCarnevali S, Petruzzelli S, Longoni B, Vanacore R, Barale R, Cipollini M, Scatena F, Paggiaro P, Celi A, Giuntini C
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID12547733
'Cigarette smoke is a mixture of chemicals having direct and/or indirect toxic effects on different lung cells. We investigated the effect of cigarette smoke on human lung fibroblasts (HFL-1) oxidation and apoptosis. Cells were exposed to various concentrations (1, 5, and 10%) of cigarette smoke extract (CSE) for 3 h, ... More
Pseudomonas aeruginosa-induced apoptosis is defective in respiratory epithelial cells expressing mutant cystic fibrosis transmembrane conductance regulator.
AuthorsCannon CL, Kowalski MP, Stopak KS, Pier GB
JournalAm J Respir Cell Mol Biol
PubMed ID12878584
'Chronic lung infection with Pseudomonas aeruginosa constitutes the most severe manifestation of cystic fibrosis, a scenario that results from defects in early clearance of the microbe. Early clearance involves epithelial cell ingestion of bacteria, rapid activation of nuclear factor-kappa B and cellular desquamation within minutes of P. aeruginosa infection, processes ... More
N- and O-glycans modulate galectin-1 binding, CD45 signaling, and T cell death.
AuthorsEarl LA, Bi S, Baum LG,
JournalJ Biol Chem
PubMed ID19920154
'Galectin-1, a beta-galactoside-binding protein highly expressed in the thymus, induces apoptosis of specific thymocyte subsets and activated T cells. Galectin-1 binds to N- and O-glycans on several glycoprotein receptors, including CD7, CD43, and CD45. Here we show that galectin-1 signaling through CD45, which carries both N- and O-glycans, is regulated ... More
Interaction of low molecular weight group IIA phospholipase A2 with apoptotic human T cells: role of heparan sulfate proteoglycans.
AuthorsBoilard E, Bourgoin SG, Bernatchez C, Poubelle PE, Surette ME
JournalFASEB J
PubMed ID12773489
'Human group IIA phospholipase A2 (hIIA PLA2) is a 14 kDa secreted enzyme associated with inflammatory diseases. A newly discovered property of hIIA PLA2 is the binding affinity for the heparan sulfate proteoglycan (HSPG) glypican-1. In this study, the binding of hIIA PLA2 to apoptotic human T cells was investigated. ... More
Mitochondrial autophagy is an HIF-1-dependent adaptive metabolic response to hypoxia.
AuthorsZhang H, Bosch-Marce M, Shimoda LA, Tan YS, Baek JH, Wesley JB, Gonzalez FJ, Semenza GL,
JournalJ Biol Chem
PubMed ID18281291
Autophagy is a process by which cytoplasmic organelles can be catabolized either to remove defective structures or as a means of providing macromolecules for energy generation under conditions of nutrient starvation. In this study we demonstrate that mitochondrial autophagy is induced by hypoxia, that this process requires the hypoxia-dependent factor-1-dependent ... More
The HER-2-targeting antibodies trastuzumab and pertuzumab synergistically inhibit the survival of breast cancer cells.
AuthorsNahta R, Hung MC, Esteva FJ
JournalCancer Res
PubMed ID15059883
Trastuzumab (herceptin) and pertuzumab (Omnitarg, 2C4) are recombinant humanized monoclonal antibodies that target different extracellular regions of the HER-2 tyrosine kinase receptor. We explored combination effects of these agents in the HER-2-overexpressing BT474 breast cancer cell line. Trastuzumab and 2C4 synergistically inhibited the survival of BT474 cells, in part, because ... More
JTV1 co-activates FBP to induce USP29 transcription and stabilize p53 in response to oxidative stress.
AuthorsLiu J, Chung HJ, Vogt M, Jin Y, Malide D, He L, Dundr M, Levens D,
JournalEMBO J
PubMed ID21285945
c-myc and p53 networks control proliferation, differentiation, and apoptosis and are responsive to, and cross-regulate a variety of stresses and metabolic and biosynthetic processes. At c-myc, the far upstream element binding protein (FBP) and FBP-interacting repressor (FIR) program transcription by looping to RNA polymerase II complexes engaged at the promoter. ... More
Drak2 regulates the survival of activated T cells and is required for organ-specific autoimmune disease.
AuthorsMcGargill MA, Choy C, Wen BG, Hedrick SM,
JournalJ Immunol
PubMed ID19017948
Drak2 is a serine/threonine kinase expressed in T and B cells. The absence of Drak2 renders T cells hypersensitive to suboptimal stimulation, yet Drak2(-/-) mice are enigmatically resistant to experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis. We show in this study that Drak2(-/-) mice were also completely resistant ... More
Cysteine Cathepsins Trigger Caspase-dependent Cell Death through Cleavage of Bid and Antiapoptotic Bcl-2 Homologues.
AuthorsDroga-Mazovec G, Bojic L, Petelin A, Ivanova S, Romih R, Repnik U, Salvesen GS, Stoka V, Turk V, Turk B,
JournalJ Biol Chem
PubMed ID18469004
As a model for defining the role of lysosomal cathepsins in apoptosis, we characterized the action of the lysosomotropic agent LeuLeuOMe using distinct cellular models. LeuLeuOMe induces lysosomal membrane permeabilization, resulting in release of lysosomal cathepsins that cleave the proapoptotic Bcl-2 family member Bid and degrade the antiapoptotic member Bcl-2, ... More
Histone H1 dephosphorylation is not a general feature in early apoptosis.
AuthorsGréen A, Sarg B, Koutzamani E, Genheden U, Lindner HH, Rundquist I,
JournalBiochemistry
PubMed ID18558721
Histone H1 is a family of nucleosomal proteins that exist in a number of subtypes. These subtypes can be modified after translation in various ways, above all by phosphorylation. Increasing levels of H1 phosphorylation has been correlated with cell cycle progression, while both phosphorylation and dephosphorylation of histone H1 have ... More
Transaldolase deficiency influences the pentose phosphate pathway, mitochondrial homoeostasis and apoptosis signal processing.
AuthorsQian Y, Banerjee S, Grossman CE, Amidon W, Nagy G, Barcza M, Niland B, Karp DR, Middleton FA, Banki K, Perl A,
JournalBiochem J
PubMed ID18498245
TAL (transaldolase) was originally described in the yeast as an enzyme of the PPP (pentose phosphate pathway). However, certain organisms and mammalian tissues lack TAL, and the overall reason for its existence is unclear. Recently, deletion of Ser(171) (TALDeltaS171) was found in five patients causing inactivation, proteasome-mediated degradation and complete ... More
Ubiquitous calpains promote caspase-12 and JNK activation during endoplasmic reticulum stress-induced apoptosis.
AuthorsTan Y, Dourdin N, Wu C, De Veyra T, Elce JS, Greer PA
JournalJ Biol Chem
PubMed ID16597616
Ubiquitously expressed mu- and m-calpain proteases are implicated in development and apoptosis. They consist of 80-kDa catalytic subunits encoded by the capn1 and capn2 genes, respectively, and a common 28-kDa regulatory subunit encoded by the capn4 gene. The regulatory subunit is required to maintain the stability and activity of mu- ... More
A FRET-based fluorogenic phosphine for live-cell imaging with the Staudinger ligation.
AuthorsHangauer MJ, Bertozzi CR,
JournalAngew Chem Int Ed Engl
PubMed ID18306205
Fluorescent phosphine probes have been used for direct imaging of various azide-bearing biomolecules with the Staudinger ligation in cell-free environments. Recently, we applied phosphine-based dyes to image azides on the surface of live cells. Notably, significant labeling above background could only be achieved using a highly negatively charged ... More
Correlating cell cycle with apoptosis in a cell line expressing a tandem green fluorescent protein substrate specific for group II caspases.
AuthorsDonahue CJ, Santoro M, Hupe D, Jones JM, Pollok B, Heim R, Giegel D
JournalCytometry
PubMed ID11746091
BACKGROUND: We describe a rapid flow cytometric assay that correlates cell cycle with apoptotic cell death in a cell line expressing a tandem green fluorescent protein (GFP). METHODS: A Jurkat cell line was transfected with a gene construct coding for constitutive expression of a tandem GFP molecule carrying a consensus ... More
A rapid method to measure beta-amyloid induced neurotoxicity in vitro.
AuthorsPatel D, Good T
JournalJ Neurosci Methods
PubMed ID17083984
Beta-amyloid (Abeta) is the primary protein component of senile plaques in Alzheimer's disease (AD) and is believed to be associated with neurotoxicity in the disease. Abeta-induced neurotoxicity is strongly dependent on its structure. The aggregation of the peptide from monomeric form to fibrils is a function of many variables including ... More
The hepatitis B virus X protein sensitizes HepG2 cells to UV light-induced DNA damage.
AuthorsLee AT, Ren J, Wong ET, Ban KH, Lee LA, Lee CG
JournalJ Biol Chem
PubMed ID16055925
Various reports have implicated the virally encoded HBx protein as a cofactor in hepatocarcinogenesis. However, direct evidence of the role of HBx as a promoter of oncogenesis in response to an initiating factor such as DNA damage remains inadequate. Here, we report the effects of HBx in HepG2 cells exposed ... More
Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging.
BACKGROUND: A highly sensitive, fast, and simple flow cytometric assay to assess human red blood cell (RBCs) viability and aging is reported. METHODS: The assay described in this report is based on the use of acetoxymethyl ester of calcein (calcein-AM), a fluorescein derivative and nonfluorescent vital dye that passively crosses ... More
Induction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (citral, limonene and caryophyllene oxide).
AuthorsMoreno ÉM, Leal SM, Stashenko EE, García LT
JournalBMC Complement Altern Med
PubMed ID30053848
Chagas Disease caused by Trypanosoma cruzi infection, is one of the most important neglected tropical diseases (NTD), without an effective therapy for the successful parasite eradication or for the blocking of the disease's progression, in its advanced stages. Due to their low toxicity, wide pharmacologic spectrum, and potential synergies, medicinal ... More
A Logic-Gated Modular Nanovesicle Enables Programmable Drug Release for On-Demand Chemotherapy.
AuthorsTang L, Yang Z, Zhou Z, Ma Y, Kiesewetter DO, Wang Z, Fan W, Zhu S, Zhang M, Tian R, Lang L, Niu G, Zhang X, Chen X
JournalTheranostics
PubMed ID30867836
It remains a major challenge to achieve precise on-demand drug release. Here, we developed a modular nanomedicine integrated with logic-gated system enabling programmable drug release for on-demand chemotherapy. ... More