冷冻细胞以样品瓶形式提供,含有 1 mL 细胞,密度为 1 x 107 个活细胞/mL,溶剂为 LV-MAX 生产培养基和 10% DMSO。细胞应直接解冻到 LV-MAX 生产培养基中。
通常将细胞置于培养瓶内,放在摇床平台上,然后放入加湿的 CO2 细胞培养箱内生长。然而,培养物体积可从 96 孔深孔板扩展到 2 L 培养瓶和生物反应器。慢病毒载体收获通常在转染后两天进行。
仅供科研使用。不可用于诊断程序。
规格
推荐培养基LV-MAX™ 生产培养基
池数量1 x 107
产品类型病毒生产细胞
数量1 mL
运输条件干冰
细胞系病毒生产细胞系
细胞类型肾脏(胚胎)
培养类型悬浮液细胞培养
Unit SizeEach
内容与储存
请储存于液氮中直至使用。请勿将细胞储存于 -80°C 条件下。
常见问题解答 (FAQ)
Can I use Viral Production Cells (Cat. No. A35347, A35827) with the AAV MAX Helper-Free AAV Production System?
The Viral Production Cells 2.0 are specifically designed to work with the other components of the AAV MAX Helper-Free AAV Production System to provide maximal adeno-associated virus (AAV) titers. Viral Production Cells could be used but we don't recommend doing this as it will reduce system performance.
What are the major differences between LV-MAX and CTS LV- MAX products?
The LV-MAX and CTS LV- MAX products have equivalent performance. In contrast to the research use only LV-MAX products, CTS LV-MAX products have an additional intended use for cell and gene therapy applications, and are complemented with a more robust regulatory support package to enable clinical therapeutic applications. This means that to the best of our ability, we keep our CTS product specifications and release requirements up-to-date in accordance with the latest regulatory guidance specific for this intended use.
What is the intended use claim for your LV-MAX and CTS LV-MAX products?
Our LV-MAX products are intended for research use only while our CTS LV- MAX products are intended for research use or manufacture of cell, gene or tissue based products. Please refer to the individual products for further details on the intended use claim.
Do you offer a lentivirus production service?
Yes. Please provide your details here (https://www.thermofisher.com/us/en/home/global/forms/life-science/lentiviral-vector-production-get-info.html) and mention your interest in our lentivirus production services. A member of our services team will contact you shortly.
With the LV-MAX Production System, are the lentiviral particles stable in packaging media?
Lentiviral particles packaged in our LV-MAX Production Medium will be stable at 4 degrees C for 24 hours and will then begin to lose activity. For long-term storage, we recommend storing at 80 degrees C.