B-27™ Plus 补充剂 (50X)
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B-27™ Plus 补充剂 (50X)
Gibco™

B-27™ Plus 补充剂 (50X)

B-27 Plus 补充剂是引用较多的神经元细胞培养产品 B-27 补充剂的下一代。它是一种无血清补充剂，当与 Neurobasal Plus 培养基配合使用时，可提供较高的原代啮齿动物来源及人干细胞来源神经元体外生存率。B-27了解更多信息
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货号数量
A358280110 mL
货号 A3582801
价格（CNY)
1,558.90
飞享价
Ends: 31-Dec-2025
2,263.00
共减 704.10 (31%)
Each
添加至购物车
数量:
10 mL
价格（CNY)
1,558.90
飞享价
Ends: 31-Dec-2025
2,263.00
共减 704.10 (31%)
Each
添加至购物车
B-27 Plus 补充剂是引用较多的神经元细胞培养产品 B-27 补充剂的下一代。它是一种无血清补充剂，当与 Neurobasal Plus 培养基配合使用时，可提供较高的原代啮齿动物来源及人干细胞来源神经元体外生存率。B-27 Plus 补充剂采用优化的配方，升级的生产工艺，以及更严格的原材料和终产品质控手段。与其他神经元细胞培养基系统相比，这些改进实现了较高的神经元细胞存活率，更高的电生理活性，以及更高的功能性成熟度。

与 Neurobasal Plus 培养基结合时，B-27 Plus 补充剂可提供：
•原代大鼠神经元比经典 B-27 补充剂和 Neurobasal 培养基至少高 50% 的存活率
• 比其他市售无血清神经元培养基系统更高的原代大鼠、小鼠和人干细胞来源神经元存活率
• 电生理活性和成熟度更高—相较于 BrainPhys™ 神经元培养基，10 天后原代啮齿动物神经元的自发活性更一致、稳定和同步
• 加速神经元突起生长并增加突触阳性刻点的数量

B-27 Plus 补充剂可作为 50X、10-mL 补充剂，无缝替代其他神经元细胞培养补充剂，如经典的 B-27 补充剂。B-27 Plus 神经元培养系统也可供购买，其中包括 B-27 Plus 补充剂和 Neurobasal Plus 培养基，可提供一种便利且极具价值的一体化订购解决方案。

仅供科研使用。不可用于诊断程序。
规格
细胞类型神经细胞
最大浓度50 X
培养类型哺乳动物细胞培养
产品线B-27™
数量10 mL
血清水平无血清
有效期12 个月
运输条件干冰
经过测试内毒素、性能、无菌性
分类无血清
形式液体
产品类型细胞培养添加剂
无菌无菌
Unit SizeEach
内容与储存
在 -5 至 -20°C 下避光储存。

常见问题解答 (FAQ)

在培养神经细胞的过程中，我可在Neurobasal培养基中加入哪些添加剂？

做为一般性的参考方案，我们推荐您使用Gibco B-27添加剂来培养神经干细胞，海马及其他类型的CNS神经元。

-对于来自PNS和CNS中的神经母细胞瘤或有丝分裂后的神经元，可添加Gibco N-2添加剂。
-对于原代胶质细胞（星形胶质细胞）或具有星形胶质细胞表型的肿瘤细胞系（星形胶质细胞和胶质细胞瘤），或少突胶质细胞，可使用Gibco G-5添加剂。

Can B-27 supplement be filtered?

Yes, the protein content in this product is high enough so filtering through a low protein binding filter as a 50X or 1X in solution should not be a problem.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?

No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is precipitate in Human Keratinocyte Growth Supplement (Cat. No. S0015, S001K) normal?

After long-term storage at -20 degrees C, Human Keratinocyte Growth Supplement (HKGS) (Cat. Nos. S0015, S001K) may contain a small amount of precipitate, which is normal. This precipitate is formed from cold insoluble material in the BPE component of the HKGS and will not affect the performance of the product. A similar precipitate may be seen in the BPE component of the HKGS Kit.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I thaw B-27 Plus Supplement (Cat. No. A3582801) in a 37 degrees C water bath or incubator?

We do not recommend thawing B-27 Supplement (Cat. No. A3582801) in a water bath or incubator. We recommend thawing the B-27 Supplement at 4 degrees C overnight.

Note: Remaining B-27 Plus Supplement (50X) may be aliquoted into working volumes and stored at -20 to -5 degrees C. Do not freeze-thaw B-27 Plus Supplement (50X) more than twice. Once thawed do not leave thawed supplement at 4 degrees C for more than two weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all B-27™ Plus 补充剂 (50X) FAQs