Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?
No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.
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Which media system should I use for primary culture of P0- or P1-derived mixed hippocampal cells?
We recommend using Neurobasal Plus Medium with B-27 Plus Supplement for long-term culture of mixed hippocampal cells. For short-term culture of a pure population of hippocampal neurons, we would recommend using Neurobasal-A Medium with B-27 Supplement.
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What is the osmolality of Neurobasal Plus Medium?
The osmolality is in the range of 215-235 mOsm/kg. The exact osmolality for your lot of the medium can be found in the Certificate of Analysis for that lot number.
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I'm currently culturing neurons. Which media system do you recommend using in conjunction with the Poly-D-Lysine matrix?
We recommend using the B-27 Plus Neuronal Culture System when culturing neurons on Poly-D-Lysine. The B-27 Plus Neuronal Culture System is a complete media system that includes the B-27 Plus Supplement and the Neurobasal Plus Medium.
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Can I thaw B-27 Supplements in a 37 degrees C water bath or incubator?
We do not recommend thawing at 37 degrees C. Best results are observed upon thawing at room temperature for approximately 2 hrs or overnight at 2-8 degrees C.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.