100% knock-in using CRISPR-Cas9—try our new TrueTag Donor DNA Kits for GFP, RFP or luciferase tagging.
TrueCut Cas9 is now available as a CTS-prototype for preclinical and translational research. Learn more »
Invitrogen™

TrueCut™ Cas9 Protein v2

货号:  A36496

相关应用:

CRISPR-Cas9 Genome Editing

| Cell & Gene Therapy

返回顶部

描述

Invitrogen TrueCut Cas9 Protein v2 is a next-generation CRISPR Cas9 protein engineered to deliver maximum editing efficiency. Features include:

• Consistently high editing efficiency in all tested cell lines including standard, immune, primary, and stem, with up to 2X higher editing efficiency in difficult targets than the competition
• High quality—manufactured under strict ISO 13485 quality standards
• Validated protocols for a large number of cell types help you achieve success faster

TrueCut Cas9 Protein v2 is recombinant Streptococcus pyogenes Cas9 (wt) protein, purified from E. coli, for genome editing with CRISPR technology. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of the CRISPR/Cas9 system. Incorporation of nuclear localization signals (NLS) aids delivery to the nucleus, increasing the rate of genomic DNA cleavage.

• Transfection-ready, using either lipid-mediated transfection reagents or electroporation
• Eliminates time-consuming cloning steps
• Available in 2 concentrations:
    --1 μg/μL for use in standard editing scenarios
    --5 μg/μL for optimization of editing conditions in more difficult scenarios such as in primary or embryonic cells, microinjection, or when screening multiple gRNA sequences at a time
    --For custom sizes or concentrations, please inquire

Options for obtaining CRISPR gRNA for use with TrueCut Cas9 Protein v2:
1. Order transfection-ready TrueGuide synthetic gRNAs
2. Generate transfection-ready gRNA in as little as four hours including template assembly with our GeneArt Precision gRNA Synthesis Kit
3. Have our Custom Services team design, synthesize, and purify in vitro (IVT) gRNA sequences for you

For Research Use Only. Not for use in diagnostic procedures.

规格

产品类型: Cas9 Protein v2
浓缩: 1μg/μL
组分数量: 10μL TrueCut Cas9 Protein v2 (1 mg/mL)
编录号: NC_002737.2:854751-858857
表达系统: E. coli
基因 ID (Entrez): 901176
基因符号: Spy_1046
基因别名: cas5, csn1
蛋白质形式: Recombinant
蛋白标记: Untagged
纯度或质量等级: Molecular Biology Grade
研究类别: Genome Editing
运输条件: Dry Ice
产品线: Invitrogen™
经证实的应用: Genome Editing

内容及储存

10 µL TrueCut Cas9 Protein v2 (1 mg/mL)
Store at -5 to -30°C.