Can I use a heat block for the AutoLys M tube incubation?
No. The AutoLys M tubes don't fit in a standard 1.5 mL or 2.0 mL heat block. We recommend incubating the tubes in the tube racks in an incubator or oven.
What kind of centrifuge do I need to spin down the AutoLys M Tubes (Cat. No. A38738)?
Any centrifuge with plate adapters will work. The AutoLys M Tube Racks fit nicely into those adapters.
Do I need to buy the AutoLys M TubeLifter (Cat. No. A37956) or AutoLys M Tube Pliers (Cat. No. A38261) to use the AutoLys M Tubes (Cat. No. A38738)?
No, the AutoLys M Tubes can be lifted manually, but the tools significantly reduce hand strain and increase ease of use as well as throughput.
Do the AutoLys M Tubes come with the MagMAX FFPE DNA/RNA Ultra Kit?
No, they have to be purchased separately together with some other products that are necessary for using the AutoLys M Tube system efficiently:
- AutoLys M Tubes and Caps (Cat. No. A38738)
- Autolys M Tube Locking Lid (Cat. No. A37954)
- AutoLys M TubeLifter (Cat. No. A37956)
- AutoLys M Tube Pliers (Cat. No. A38261)
- AutoLys M Tube Racks (Cat. No. A37955)
How does using the the AutoLys M Tube system help in increase the DNA/RNA yield from FFPE samples?
The FFPE-associated wax and debris are held in the upper chamber of the AutoLys M tube while the lysate passes through. Nucleic acids can then be purified from the clarified lysate. The AutoLys M tubes thus reduce manipulation of the sample eliminating the need to pellet the tissue and wash, so tissue loss is minimized
Can I use the AutoLys M Tube system with any of your FFPE sample prep kits or has it only been optimized for the MagMAX FFPE DNA/RNA Ultra Kit?
The AutoLys M Tube system has only been tested with the MagMAX FFPE DNA/RNA Ultra Kit, but it may also work with our RecoverAll Total Nucleic Acid Isolation Kit for FFPE .
I am planning on using your MagMAX FFPE DNA/RNA Ultra Kit. I don't want to perform a deparaffinization step using organic solvents, is there an alternative reagent or method I can use?
Yes, we offer the AutoLys M Tube system, which creates cleared lysates from FFPE tissue samples without the need for deparaffinization or wash steps, while increasing DNA and RNA yields.