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View additional product information for Genexus™ Library Strips 1 and 2-AS - FAQs (A40252)
19 product FAQs found
Remove the templating strips from their boxes in the refrigerator or freezer and get them ready them for loading in the sequencer by equilibrating to/thawing at room temperature for 30 mins. Confirm that the strip contents are completely thawed before loading in the sequencer. If you are delayed in loading, keep the thawed strips on ice or at 4 degrees C until you load them in the sequencer.
Genexus Strip 3B‑GX5 contain magnetic beads in one or two positions, yellow or brown in color, that sometimes get trapped in the upper “keyhole” of the tube. Dislodge these beads from the keyhole before installing the strip in the sequencer using the following procedure for each strip:
- Invert the strip 3-4 times to dislodge beads that are trapped in the keyholes.
- To remove any remaining beads and liquid from the keyholes, grasp the strip at one end with the strip seal facing up, then swing the strip with a rapid, downward centrifugal arm motion, ending with a sharp wrist-flick.
- Grasp the strip at the other end, then repeat the centrifugal motion.
- Check tube positions for significant amounts of beads that are still trapped in keyholes and then repeat the centrifugal motion, if needed. It is acceptable if a few beads remain in the keyhole or on the tube wall, but most should be either in suspension or in a pellet at the bottom of the tube.
Genexus Templating Strips 3B-GX5 and 4-GX5 (Cat. No. A49782) has new Genexus Strip 3B that has the chemistry modified to improve templating and sequencing of GC rich regions.
Genexus Templating Strips 3B-GX5 and 4-GX5 is specifically designed for use with the Oncomine Myeloid GX v2 assay. For all other Genexus assays, please use Genexus Templating Strips 3-GX5 and 4 (Cat. No. A40263).
Genexus Templating Strips 3B-GX5 and 4-GX5 provides 8 pairs of strips per kit - Genexus Templating Strips 3B-GX5 (Part No. A47712) (Orange, 8 strips) and Genexus Templating Strips 4-GX5 (Part No. A46816) (Yellow, 8 strips). Each pair of strips is required to perform automated template preparation and chip loading of two GX5 chips using the Genexus Integrated Sequencer.
Genexus Templating Strips 3B-GX5 (Part No. A47712) (Orange, 8 strips) ships on a gel pack and should be stored at 2 to 8 degrees C. Genexus Templating Strips 4-GX5 (Part No. A46816) (Yellow, 8 strips) ships on dry ice and should be stored at -30 to -10 degrees C.
Detection of somatic variants with the Oncomine Myeloid Research Assay GX has been verified down to 5% allele frequency.
Here is a list of kits that are compatible with Oncomine Myeloid Research Assay GX:
Nucleic acid isolation - RNA samples
- MagMAX mirVana Total RNA Isolation Kit, Cat. No. A27828
- PureLink Total RNA Blood Kit, Cat. No. K156001
- RNaseZap RNase Decontamination Solution, Cat. No. AM9780, AM9782, AM9784
Nucleic acid isolation - DNA samples
- MagMAX DNA Multi-Sample Ultra 2.0 Kit, Cat. No. A36570
- PureLink Genomic DNA Mini Kit, Cat. No. K1820-01
The recommended minimum sample input is 27.75 ng of DNA (1.11 ng/µL minimum concentration) and 14.25 ng of RNA (0.95 ng/µL minimum concentration).
The recommended controls are:
DNA Controls:
- Seraseq® Myeloid Mutation DNA Mix, Cat. No. 0710-0408 (SeraCare®)
- Myeloid DNA Reference Standard, Cat. No. HD829 (Horizon™)
- AcroMetrix™ Oncology Hotspot Control, Cat. No. 969056
RNA Controls:
- Universal Human Reference RNA, Cat. No. 740000 (Agilent™)
- Seraseq® Myeloid Fusion RNA Mix, Cat. No. 0710-0407 (SeraCare®)
Multiplex sequencing of up to 8 Oncomine Myeloid Research Assay GX samples (8 DNA+ 8 RNA) per lane on a GX5 Chip can be performed in a single run. There are 4 lanes on the GX5 Chip, so 32 total samples (32 DNA+ 32 RNA) can be run on one GX5 Chip (8 samples per run).
The Oncomine Myeloid Research Assay GX (Cat. No. A47857) includes the 2-pool DNA panel, the 1-pool RNA panel, Genexus Strip 1, and Genexus Strip 2-AS. The DNA panel is provided in two packs of 8 tubes (4 tubes of Myeloid DNA Pool 1 and 4 tubes of Myeloid DNA Pool 2 per pack). The RNA panel is provided in one pack of 8 tubes (Myeloid RNA Pool 1). Each primer pool in the panel is provided in pairs of tubes, where each tube pair contains one tube with primers in position 1 and one empty uncapped tube in position 2. Three 8-strip packs of the Genexus Strip 1 and Genexus Strip 2-AS (24 total of each strip) are provided with each kit.
The contents of each Oncomine Myeloid Research Assay GX kit are sufficient for up to 32 samples (32 x 3‑pool reactions: 32 x 2‑pool DNA reactions and 32 x 1‑pool RNA reactions).
The Oncomine Myeloid Research Assay GX includes 3 pools of Ion AmpliSeq oligonucleotide primers (a 2-pool DNA panel and a 1-pool RNA panel).
The Oncomine Myeloid Research Assay GX targets key genes and fusions associated with major myeloid disorders, including acute myeloid leukemia (AML), myeloid dysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myeloid leukemia (CML), chronic myelomonocytic leukemia (CMML), and juvenile myelomonocytic leukemia (JMML). The DNA panel comprises 40 key genes while the RNA panel includes a broad fusion panel of 29 driver genes, enabling detection over 600 unique fusion isotypes. See flyer for gene targets (https://assets.thermofisher.com/TFS-Assets/CSD/Flyers/Oncomine_myeloid_assay_gx_flyer.pdf).
The Oncomine Myeloid Research Assay GX is a comprehensive targeted next-generation sequencing (NGS) assay designed for sensitive detection of myeloid disorder-associated DNA mutations and fusion transcripts in blood and bone marrow samples.
Remove the library strips from their boxes in the refrigerator or freezer, and prepare them for loading in the sequencer.
- Genexus Strip 1 needs to equilibrate to room temperature for 30 mins.
- Genexus Strip 2-AS needs to thaw on ice for at least 30 mins. Keep the strips on ice until you load them in the sequencer. Confirm that the strip contents are completely thawed before installing in the sequencer.
Genexus Strip 1 contains magnetic beads in one or two positions, yellow or brown in color, that sometimes get trapped in the upper "keyhole" of the tube. Dislodge these beads from the keyhole by inverting each strip 3-4 times, before installing the strip in the sequencer.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
Genexus Strip 1 (red) should be stored at 4 degrees C, and Genexus Strip 2-AS (blue) should be stored at -20 degrees C.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
Genexus Library Strips 1 and 2-AS for standard Ion AmpliSeq library-based chemistry provides eight pairs of strips/kit, to perform automated library preparation using the Genexus Integrated Sequencer.
Each library strip pair is needed to create an Ion AmpliSeq library and supports up to 4 barcodes.
So 8 strip pairs x 4 = up to 32 DNA or RNA samples/run
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.
Genexus Library Strips 1 and 2-AS for standard Ion AmpliSeq library-based chemistry provides eight pairs of strips/kit, to perform automated library preparation using the Genexus Integrated Sequencer.
Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.