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View additional product information for MagMAX™ Viral/Pathogen Ultra Nucleic Acid Isolation Kit - FAQs (A42356)
14 product FAQs found
Some bead carryover is common and has not been documented to affect downstream assays. The most common cause of bead carryover is too much input sample. The best way to determine if the sample amount is the cause of the issue is to run PBS with Xeno (artificial DNA/RNA) in the lysis buffer and then check if there is still bead carryover. If that is not the cause, the initial sample may require special processing, such as bead beating or liquification. If there is still bead carryover without the original sample, and in particular, if the carryover is present in the same wells for multiple runs, then this may be an instrument alignment issue.
Plastic consumables need to be purchased separately in order to use the MagMAX reagents on the MagMAX Express-96 or a KingFisher instrument. The particular consumables and the amount required will vary by kit and protocol so please check the user guide for the particular MagMAX kit to be sure that all required plastic consumables are available prior to sample processing.
The best place to download KingFisher Flex and KingFisher Duo Prime protocols for a particular MagMAX kit is the relevant MagMAX kit product page. The Bindlt script protocol (.bdz) files can be found under the Documents>Product literature section.
Yes, reaction volumes for MagMAX kits can be scaled up. In general, the amount of MagMAX reagents should be scaled up proportionally for the increase in sample input amount. Please see the specific kit user guide for any recommendation. If none are included, then optimization may be necessary.
Addition of BME (2-mercaptoethanol) to the lysis buffer of MagMAX kits is fine. This can be beneficial with more RNase-rich samples like certain types of tissues.
In general, we recommend adding 0.3 µL BME per 100 µL of lysate.
We recommend using the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit (Cat. No. A42352) for extracting total nucleic acid from easily lysed viruses and Gram-negative bacteria. For difficult-to-lyse samples like Gram-positive bacteria and fungi, we recommend using the MagMAX Viral/Pathogen Ultra Kit (Cat. No. A42356).
It takes <60 min on instrument time from sample to extracted nucleic acid for up to 96 samples.
With the MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation Kit, nucleic acid has been successfully detected from as low as 50 viral copies in the starting sample.
With the MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation Kit, nucleic acid has been successfully detected from as low as 1000 cfu of bacteria and yeast/fungi in the starting sample.
Multiple targets each of (-)ssRNA virus, (+)ssRNA virus, ssDNA virus, dsDNA virus, Gram negative bacteria, Gram positive bacteria, yeast, yeast-like fungi, and fungi have been tested with the MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation Kit.
Human biofluid samples (e.g., blood, serum, plasma, urine, CSF, lavage, saliva), samples stored in transport media (e.g., BD Universal Viral Transport (UVT) Media), and bacteriophage culture are compatible with the MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation Kit.
The following downstream analysis are compatible with the MagMAX Viral/Pathogen Nucleic Acid Isolation Kit and MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation Kit: PCR, qPCR, qRT-PCR, OpenArray analysis, microarray analysis, and next-generation sequencing.
The MagMAX Viral/Pathogen and MagMAX Viral/Pathogen Ultra nucleic acid isolation kits are compatible with all KingFisher instruments (Flex, Duo Prime, and Presto) and can also be used for manual processing in 96-well plates.
For the 96-deep well plate format: 200-400 µL sample volume input (200 µL for whole blood
For the 24-deep well plate format: 500-2,000 µL sample volume input (1,000 µL for whole blood)