AAV-MAX Helper-Free AAV Production System Kit
AAV-MAX Helper-Free AAV Production System Kit
Gibco™

AAV-MAX Helper-Free AAV Production System Kit

The AAV-MAX Helper-Free AAV Production System Kit is a scalable, high-titer platform for suspension AAV production.
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Catalog NumberQuantity
A51217Sufficient for 1 L of Culture
Catalog number A51217
Price (CNY)
-
Quantity:
Sufficient for 1 L of Culture

The Gibco AAV-MAX Helper-Free AAV Production System is a scalable, high-titer platform for suspension AAV production. It is based on a high-density suspension culture of clonal 293F-derived Viral Production Cells 2.0 in Viral Production Medium. Optimal viral titers are mediated by our advanced lipid nanoparticle transfection reagent in combination with a novel production enhancer. The components are designed to work synergistically, resulting in maximal titers and eliminating the need to optimize reagents and protocols.

Features of the AAV-MAX Helper-Free AAV Production System include:

  • Components work synergistically to produce high AAV titers greater than 5 x 1010 vg/mL (unpurified)
  • Suspension-based system has scalable protocols from shake flask to bioreactor scale
  • Simplified workflow—streamlined protocol using helper virus-free triple transfection
  • Clonal, 293F-derived, high-producer cell line
  • Robust transfection protocols are adaptable to large-scale transfection
  • The components included in the AAV-MAX System Kit are:
    • 1 vial of Viral Production Cells 2.0
    • 1 L of Viral Production Medium
    • 1 AAV-MAX Transfection Kit for 1 L of production
    • 100 mL Viral-Plex Complexation Buffer
    • 100 mL AAV-MAX Lysis Buffer
    • 100 mL GlutaMAX Supplement

All components are also available for purchase separately.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineViral Production Cells 2.0
Cell TypeKidney (Embryonic)
For Use With (Application)AAV Production
Product LineAAV-MAX
Product TypeAAV Production System
QuantitySufficient for 1 L of Culture
Shelf Life12 months from date of manufacture
Culture TypeSuspension Cell Culture
SterilitySterile
Unit SizeEach
Contents & Storage
• 1 vial Viral Production Cells 2.0
• 1 L Viral Production Medium
• 1 AAV-MAX Transfection Kit for 1 L production
• 100 mL Viral-Plex Complexation Buffer
• 100 mL AAV-MAX Lysis Buffer
• 100 mL GlutaMAX Supplement

Storage conditions vary

Frequently asked questions (FAQs)

Are the components in the AAV-MAX Helper-Free AAV Production System available separately, or are they only sold as a kit?

The Viral Production Medium, Viral Production Cells 2.0, AAV-MAX Transfection Kit, Viral-Plex Complexation Buffer, and AAV-MAX Lysis Buffer are sold individually and here are the stand-alone catalog numbers:
- Viral Production Medium: Cat. No. A4817901, A4817902, A4817903
- Viral Production Cells 2.0: Cat. No. A49784, A51218
- AAV-MAX Transfection Kit: Cat. No. A50515, A50516
- Viral-Plex Complexation Buffer: A4983901
- AAV-MAX Lysis Buffer: A50520

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use other transfection reagents, such as PEI (polyethylenimine), in the AAV-MAX Helper-Free AAV Production System?

The AAV-MAX Transfection Reagent, AAV-MAX Tranfection Booster, and AAV-MAX Enhancer are specifically designed to work synergistically with the Viral Production Medium and Viral Production Cells 2.0 to provide maximum titers. Mixing and matching of reagents is not recommended, as this will decrease the overall performance.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

How scalable is the AAV-MAX transfection protocol?

The AAV-MAX transfection protocol is scalable from 24 deep-well plates to shake flasks to bioreactors. The user guide provides guidance on this.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Are the LV-MAX Enhancer and AAV-MAX Enhancer different?

Yes, these are different, and each is optimized for its intended use.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Will I see a drop in adeno-associated virus (AAV) titer if I don't use the AAV-MAX Enhancer with the AAV-MAX Helper-Free AAV Production System?

The AAV-MAX Enhancer is important for boosting AAV production. If the enhancer is not used, it will reduce system performance by 50% or more.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

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