Fetal Bovine Serum, Value
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Fetal Bovine Serum, Value
Fetal Bovine Serum, Value
Fetal Bovine Serum, Value
Fetal Bovine Serum, Value
Citations & References (1)
Gibco™

Fetal Bovine Serum, Value

Green features
Gibco fetal bovine sera offer an excellent value for basic cell culture, specialty research, and specific assays.
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Catalog NumberQuantityFormatHeat InactivatedGamma-irradiated
A5256701500 mLBottleNoNo
A5256501500 mLBottleNoYes
A5256901500 mLBottleYesYes
Catalog number A5256701
Price (CNY)
4,943.00
Each
Add to cart
Quantity:
500 mL
Format:
Bottle
Heat Inactivated:
No
Gamma-irradiated:
No
Price (CNY)
4,943.00
Each
Add to cart

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements.

Sera category: Premium

  • Use for general cell culture with common cell lines
  • Low endotoxin and hemoglobin levels
  • Endotoxin level: <10 EU/mL
  • Hemoglobin level: <25 mg/dL
  • Origin: refer to CoA for specific origin. All products may not be available in all regions due to importation regulations.
  • US Department of Agriculture (USDA) safety tested for the absence of bluetongue and akabane from countries that are USDA-approved for importation into the US

Gibco serum delivers

  • ISO 13485–certified; processed in FDA registered facilities
  • Triple-filtered at 0.1 μm
  • Gibco bottle easier to use in hood; helps reduce risk of contamination and improve consistency
  • Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook

* FBS import requirements vary globally, and acceptance depends on whether competent authorities allow import from a specific country of origin. Please check applicable regulations for the export of FBS and any FBS-containing products (e.g., cultured cells). Contact your Thermo Fisher Scientific representative for support.

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Specifications
DescriptionGibco A5256701
Endotoxin Concentration≤20 EU/mL
For Use With (Application)*FBS import requirements vary globally, and acceptance depends on whether competent authorities allow import from a specific country of origin. Please check applicable regulations for the export of FBS and any FBS-containing products (e.g., cultured cells). Contact your Thermo Fisher Scientific representative for support.
Gamma-irradiatedNo
Green FeaturesLess waste
Heat InactivatedNo
Hemoglobin Concentration≤25 mg/dL
ProcessingTriple Filtered at 0.1 μm
Purity or Quality GradeValue
Shelf Life5 Years
Shipping ConditionDry Ice
SpeciesCattle/Bovine
Tested ForUp to 30 quality tests including 9CFR virus testing* endotoxin, performance (*Please note: If manufactured in the UK, Full 9CFR except for Rabies and BlueTongue Virus which is tested via EMA)
USDA Safety Testing IncludedUSDA Safety Testing Performed for Mexican and Australian origins
AgeFetal
Country of OriginRefer to CoA for specific origin*
FormLiquid
FormatBottle
Product TypeFetal Bovine Serum
Quantity500 mL
Serum TreatmentStandard (Sterile-filtered)
SterilitySterile
Sterilization MethodTriple-filtered, 0.1 μm
Unit SizeEach
Contents & Storage
Store at <-10°C.

Frequently asked questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How much of the total protein measured in Fetal Bovine Serum (heat inactivated or otherwise) is attributed to albumin?

Our CoAs only capture the total protein content not specifically the amount attributed to albumin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How much Fetal Bovine Serum (FBS) do I need to add to my medium?

FBS is added to culture medium at a concentration of 2-10% to provide attachment factors, nutrients, and hormones for mammalian cells, as well as to be a buffer against disruptions like pH changes and endotoxins. FBS has significant amounts of embryonic growth promoting factors like hormones, carrier proteins, and macromolecular proteins. It also has low levels of antibodies and other growth-inhibiting components. For most basal medium, 10% FBS is used. Some applications require up to 20% supplementation, so researchers need to determine what is optimal for their specific application and cell line.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Fetal Bovine Serum, Value FAQs

Citations & References (1)

Citations & References
Abstract
Non-viral-mediated gene transfer of OX40 ligand for tumor immunotherapy.
Authors:Rakitina OA,Kuzmich AI,Bezborodova OA,Kondratieva SA,Pleshkan VV,Zinovyeva MV,Didych DA,Sass AV,Snezhkov EV,Kostina MB,Koksharov MO,Alekseenko IV
Journal:Frontiers in immunology
PubMed ID:39007148
BACKGROUND: Immune checkpoint blockade (ICB) is rapidly becoming a standard of care in the treatment of many cancer types. However, the subset of patients who respond to this type of therapy is limited. Another way to promote antitumoral immunity is the use of immunostimulatory molecules, such as cytokines or T ... More