Q: RiboPure RNA纯化试剂盒的柱分离临界尺寸是多少？

柱分离临界尺寸为200 nt。RiboPure试剂盒不适用于回收miRNA。我们推荐使用mirVana试剂盒、TRIzol Plus RNA纯化试剂盒或PureLink RNA微量提取试剂盒分离miRNA。

Q: PureLink RNA小量提取试剂盒和RiboPure RNA纯化试剂盒在技术方面有何差异？

PureLink RNA小量提取试剂盒提供快速的总RNA柱纯化，无需有机溶解（苯酚/氯仿）。单次提取可获得多达1,000 µg的纯化RNA。RiboPure RNA纯化试剂盒结合了苯酚/硫氰酸胍溶液和玻璃纤维过滤纯化法。

Q: I'm getting poor quality/poor purity of RNA after isolation with the RiboPure RNA Purification kit. What could be the cause of this?

Poor quality/poor purity RNA is typically due to the following: - Partially degraded RNA: Use fresh tissue or cells, or use RNAlater Stabilization Solution if tissue cannot be frozen immediately. - DNA contamination: If the sample contains organic solvents, strong buffers, or an alkaline solution, we recommend performing phase separation at 8 degrees C. - A low A260/A280 ratio (<1.6): This indicates that an insufficient amount of the TRI Reagent was used. Incubation of the homogenate at room temperature for 5 minutes can help nucleoproteins dissociate from RNA. Phenol contamination could also cause a low absorbance ratio.

Q: What is the column cut-off size for the RiboPure RNA Purification Kits?

The column cut-off size is 200 nt. Using a RiboPure kit is not appropriate for miRNA recovery. For miRNA isolation, we would suggest using a mirVana kit, the TRIzol Plus RNA Purification Kit, or the PureLink RNA Micro Kit.

Q: What are the differences in technology between the PureLink RNA Mini Kit and RiboPure RNA Purification Kits?

The PureLink RNA Mini Kit provides rapid column-based purification of total RNA, without organic lysis (phenol/chloroform). You can obtain up to 1,000 µg of purified RNA from a single extraction. The RiboPure RNA Purification Kits combine a phenol/guanidine thiocyanate solution with a glass-fiber filter purification method.

Q: What is the composition of the elution buffer included in the RiboPure RNA Purification Kit (Cat. No. AM1924)?

The elution buffer composition is proprietary.

Question 1

使用RiboPure RNA纯化试剂盒分离RNA后，RNA质量/纯度很差，可能有哪些原因？

Accepted Answer

RNA质量/纯度差通常是由于以下原因：

•RNA部分降解：应使用新鲜的组织或细胞，或在无法立即冻存组织的情况下使用RNAlater溶液保存样品。
•DNA污染：如果样品含有机溶剂、强力缓冲液或碱性溶液，我们推荐在8°C下进行液相分离。
•A260/A280比值低（<1.6）：这表示TRI Reagent用量不足。在室温下孵育匀浆液5分钟，有助于核蛋白从RNA上解离。苯酚污染也会引起吸光度比值降低。

Question 2

RiboPure RNA纯化试剂盒的柱分离临界尺寸是多少？

Accepted Answer

柱分离临界尺寸为200 nt。RiboPure试剂盒不适用于回收miRNA。我们推荐使用mirVana试剂盒、TRIzol Plus RNA纯化试剂盒或PureLink RNA微量提取试剂盒分离miRNA。

Question 3

PureLink RNA小量提取试剂盒和RiboPure RNA纯化试剂盒在技术方面有何差异？

Accepted Answer

PureLink RNA小量提取试剂盒提供快速的总RNA柱纯化，无需有机溶解（苯酚/氯仿）。单次提取可获得多达1,000 µg的纯化RNA。RiboPure RNA纯化试剂盒结合了苯酚/硫氰酸胍溶液和玻璃纤维过滤纯化法。

Question 4

I'm getting poor quality/poor purity of RNA after isolation with the RiboPure RNA Purification kit. What could be the cause of this?

Accepted Answer

Poor quality/poor purity RNA is typically due to the following:

- Partially degraded RNA: Use fresh tissue or cells, or use RNAlater Stabilization Solution if tissue cannot be frozen immediately.
- DNA contamination: If the sample contains organic solvents, strong buffers, or an alkaline solution, we recommend performing phase separation at 8 degrees C.
- A low A260/A280 ratio (<1.6): This indicates that an insufficient amount of the TRI Reagent was used. Incubation of the homogenate at room temperature for 5 minutes can help nucleoproteins dissociate from RNA. Phenol contamination could also cause a low absorbance ratio.

Question 5

What is the column cut-off size for the RiboPure RNA Purification Kits?

Accepted Answer

The column cut-off size is 200 nt. Using a RiboPure kit is not appropriate for miRNA recovery. For miRNA isolation, we would suggest using a mirVana kit, the TRIzol Plus RNA Purification Kit, or the PureLink RNA Micro Kit.

Question 6

What are the differences in technology between the PureLink RNA Mini Kit and RiboPure RNA Purification Kits?

Accepted Answer

The PureLink RNA Mini Kit provides rapid column-based purification of total RNA, without organic lysis (phenol/chloroform). You can obtain up to 1,000 &micro;g of purified RNA from a single extraction. The RiboPure RNA Purification Kits combine a phenol/guanidine thiocyanate solution with a glass-fiber filter purification method.

Question 7

What is the composition of the elution buffer included in the RiboPure RNA Purification Kit (Cat. No. AM1924)?

Accepted Answer