20X Bolt™ MES SDS 运行缓冲液
20X Bolt™ MES SDS 运行缓冲液
Invitrogen™

20X Bolt™ MES SDS 运行缓冲液

Bolt MES SDS 运行缓冲液 (20X) 专为在 Bolt Bis-Tris 凝胶上进行蛋白电泳而配制。推荐用于分离中小型分子量的蛋白了解更多信息
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货号数量
B0002500 mL
B0002025 L
货号 B0002
价格(CNY)
1,092.00
Each
添加至购物车
数量:
500 mL
价格(CNY)
1,092.00
Each
添加至购物车
Bolt MES SDS 运行缓冲液 (20X) 专为在 Bolt Bis-Tris 凝胶上进行蛋白电泳而配制。推荐用于分离中小型分子量的蛋白。

选用合适的缓冲液可实现理想的蛋白分离
Bolt MES SDS 运行缓冲液和 Bolt MOPS SDS 运行缓冲液可与 Bolt Bis-Tris 凝胶配合使用。MES 具有更低的 pKa,使得 MES SDS 电泳缓冲液的速度比 MOPS SDS 电泳缓冲液更快。不同缓冲液的离子迁移效应的差异会影响积层,进而导致蛋白质分离范围差异。使用 MOPS 缓冲液可使蛋白质电泳速度比使用 MES 缓冲液时慢

在 Bolt Bis-Tris Plus 预制胶上使用 MES 和 MOPS 比较蛋白质的迁移模式

请参阅所有可用于 SDS-PAGE 的缓冲液和试剂

仅供科研使用。不可用于诊断程序。
规格
化学名称或材料MES SDS Running Buffer
建议的储存条件MES SDS Running Buffer (20X).

Store at room temperature.

Shelf-life is 6 months, when stored properly.
最大浓度20X
适用于(设备)Bolt
产品线Bolt
数量500 mL
Unit SizeEach

常见问题解答 (FAQ)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What are the storage conditions for Bolt gels?

Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the shelf life for Bolt gels?

The shelf life for Bolt gels is similar to that for NuPAGE gels: 12 months from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.