Autoantigen Golgin-97, an effector of Arl1 GTPase, participates in traffic from the endosome to the trans-golgi network.
AuthorsLu L, Tai G, Hong W
JournalMol Biol Cell
PubMed ID15269279
'The precise cellular function of Arl1 and its effectors, the GRIP domain Golgins, is not resolved, despite our recent understanding that Arl1 regulates the membrane recruitment of these Golgins. In this report, we describe our functional study of Golgin-97. Using a Shiga toxin B fragment (STxB)-based in vitro transport assay, ... More
Caveolin regulates endocytosis of the muscle repair protein, dysferlin.
AuthorsHernández-Deviez DJ, Howes MT, Laval SH, Bushby K, Hancock JF, Parton RG,
JournalJ Biol Chem
PubMed ID18096699
'Dysferlin and Caveolin-3 are plasma membrane proteins associated with muscular dystrophy. Patients with mutations in the CAV3 gene show dysferlin mislocalization in muscle cells. By utilizing caveolin-null cells, expression of caveolin mutants, and different mutants of dysferlin, we have dissected the site of action of caveolin with respect to dysferlin ... More
Specialized cortical subnetworks differentially connect frontal cortex to parahippocampal areas.
AuthorsHirai Y, Morishima M, Karube F, Kawaguchi Y,
JournalJ Neurosci
PubMed ID22302828
'How information is manipulated and segregated within local circuits in the frontal cortex remains mysterious, in part because of inadequate knowledge regarding the connectivity of diverse pyramidal cell subtypes. The frontal cortex participates in the formation and retrieval of declarative memories through projections to the perirhinal cortex, and in procedural ... More
AuthorsGaus K, Le Lay S, Balasubramanian N, Schwartz MA
JournalJ Cell Biol
PubMed ID16943184
'The properties of cholesterol-dependent domains (lipid rafts) in cell membranes have been controversial. Because integrin-mediated cell adhesion and caveolin both regulate trafficking of raft components, we investigated the effects of adhesion and caveolin on membrane order. The fluorescent probe Laurdan and two-photon microscopy revealed that focal adhesions are highly ordered; ... More
Metastatic potential of mouse Lewis lung cancer cells is regulated via ganglioside GM1 by modulating the matrix metalloprotease-9 localization in lipid rafts.
AuthorsZhang Q, Furukawa K, Chen HH, Sakakibara T, Urano T, Furukawa K
JournalJ Biol Chem
PubMed ID16636068
'To analyze mechanisms for cancer metastasis, we established high metastatic sublines from mouse Lewis lung cancer (P29) by repeated injection. Sublines established from the two subclones H7 and C4 commonly exhibited increased proliferation and invasion activity and reduced expression of ganglioside GM1, although they showed different preferences in their target ... More
Quantitative and dynamic assessment of the contribution of the ER to phagosome formation.
AuthorsTouret N, Paroutis P, Terebiznik M, Harrison RE, Trombetta S, Pypaert M, Chow A, Jiang A, Shaw J, Yip C, Moore HP, van der Wel N, Houben D, Peters PJ, de Chastellier C, Mellman I, Grinstein S,
JournalCell
PubMed ID16213220
'Phagosomes were traditionally thought to originate from an invagination and scission of the plasma membrane to form a distinct intracellular vacuole. An alternative model implicating the endoplasmic reticulum (ER) as a major component of nascent and maturing phagosomes was recently proposed (Gagnon et al., 2002). To reconcile these seemingly disparate ... More
Modulation of bulbospinal rostral ventral lateral medulla neurons by hypoxia/hypercapnia but not medullary respiratory activity.
AuthorsBoychuk CR, Woerman AL, Mendelowitz D,
JournalHypertension
PubMed ID23108653
'Although sympathetic vasomotor discharge has respiratory modulation, the site(s) responsible for this cardiorespiratory interaction is unknown. One likely source for this coupling is the rostral ventral lateral medulla (RVLM), where presympathetic neurons originate in close apposition to respiratory neurons. The current study tested the hypothesis that RVLM bulbospinal neurons are ... More
Raft composition at physiological temperature and pH in the absence of detergents.
AuthorsAyuyan AG, Cohen FS,
JournalBiophys J
PubMed ID17993486
'Biological rafts were identified and isolated at 37 degrees C and neutral pH. The strategy for isolating rafts utilized membrane tension to generate large domains. For lipid compositions that led only to microscropically unresolvable rafts in lipid bilayers, membrane tension led to the appearance of large, observable rafts. The large ... More
Anatomic and Molecular Development of Corticostriatal Projection Neurons in Mice.
AuthorsSohur US, Padmanabhan HK, Kotchetkov IS, Menezes JR, Macklis JD,
JournalCereb Cortex
PubMed ID23118198
'Corticostriatal projection neurons (CStrPN) project from the neocortex to ipsilateral and contralateral striata to control and coordinate motor programs and movement. They are clinically important as the predominant cortical population that degenerates in Huntington''s disease and corticobasal ganglionic degeneration, and their injury contributes to multiple forms of cerebral palsy. Together ... More
Acute and chronic changes in cholesterol modulate Na-Pi cotransport activity in OK cells.
AuthorsBreusegem SY, Halaihel N, Inoue M, Zajicek H, Lederer E, Barry NP, Sorribas V, Levi M
JournalAm J Physiol Renal Physiol
PubMed ID15769937
'We previously showed an inverse correlation between membrane cholesterol content and Na-P(i) cotransport activity during the aging process and adaptation to alterations in dietary P(i) in the rat (Levi M, Jameson DM, and van der Meer BW. Am J Physiol Renal Fluid Electrolyte Physiol 256: F85-F94, 1989). The purpose of ... More
Correlated fluorescence-atomic force microscopy of membrane domains: structure of fluorescence probes determines lipid localization.
AuthorsShaw JE, Epand RF, Epand RM, Li Z, Bittman R, Yip CM
JournalBiophys J
PubMed ID16361347
'Coupling atomic force microscopy (AFM) with high-resolution fluorescence microscopy is an attractive means of identifying membrane domains by both physical topography and fluorescence. We have used this approach to study the ability of a suite of fluorescent molecules to probe domain structures in supported planar bilayers. These included BODIPY-labeled ganglioside, ... More
Interleukin-15 rescues tolerant CD8+ T cells for use in adoptive immunotherapy of established tumors.
'CD8+ T cells can mediate eradication of established tumors, and strategies to amplify tumor-reactive T-cell numbers by immunization or ex vivo expansion followed by adoptive transfer are currently being explored in individuals with cancer. Generating effective CD8+ T cell-mediated responses to tumors is often impeded by T-cell tolerance to relevant ... More
Tracing from fat tissue, liver, and pancreas: a neuroanatomical framework for the role of the brain in type 2 diabetes.
AuthorsKreier F, Kap YS, Mettenleiter TC, van Heijningen C, van der Vliet J, Kalsbeek A, Sauerwein HP, Fliers E, Romijn JA, Buijs RM
JournalEndocrinology
PubMed ID16339209
'The hypothalamus uses hormones and the autonomic nervous system to balance energy fluxes in the body. Here we show that the autonomic nervous system has a distinct organization in different body compartments. The same neurons control intraabdominal organs (intraabdominal fat, liver, and pancreas), whereas sc adipose tissue located outside the ... More
TAG1 regulates the endocytic trafficking and signaling of the semaphorin3A receptor complex.
AuthorsDang P, Smythe E, Furley AJ,
JournalJ Neurosci
PubMed ID22836270
'Endocytic trafficking of membrane proteins is essential for neuronal structure and function. We show that Transient Axonal Glycoprotein 1 (TAG1 or CNTN2), a contactin-related adhesion molecule, plays a central role in the differential trafficking of components of the semaphorin3A (Sema3A) receptor complex into distinct endosomal compartments in murine spinal sensory ... More
High-resolution mapping of anatomical connections in marmoset extrastriate cortex reveals a complete representation of the visual field bordering dorsal V2.
AuthorsJeffs J, Federer F, Ichida JM, Angelucci A,
JournalCereb Cortex
PubMed ID22523183
The primate visual cortex consists of many areas. The posterior areas (V1, V2, V3, and middle temporal) are thought to be common to all primate species. However, the organization of cortex immediately anterior to area V2 (the ... More
Reciprocal Homosynaptic and Heterosynaptic Long-Term Plasticity of Corticogeniculate Projection Neurons in Layer VI of the Mouse Visual Cortex.
AuthorsArami MK, Sohya K, Sarihi A, Jiang B, Yanagawa Y, Tsumoto T,
JournalJ Neurosci
PubMed ID23637171
Most neurons in layer VI of the visual cortex project to the dorsal lateral geniculate nucleus (dLGN). These corticogeniculate projection neurons (CG cells) receive top-down synaptic inputs from upper layers (ULs) and bottom-up inputs from the underlying white matter (WM). Use-dependent plasticity of these synapses in layer VI of the ... More
Connectional heterogeneity of the ventral part of the macaque area 46.
AuthorsGerbella M, Borra E, Tonelli S, Rozzi S, Luppino G,
JournalCereb Cortex
PubMed ID22499799
We found that the ventral part of the prefrontal area 46 (46v) is connectionally heterogeneous. Specifically, the rostral part (46vr) displayed an almost exclusive and extensive intraprefrontal connectivity and extraprefrontal connections limited to area 24 and inferotemporal areas. In contrast, the caudal part (46vc) mostly displayed intraprefrontal connectivity with ventrolateral ... More
Hierarchical connectivity and connection-specific dynamics in the corticospinal-corticostriatal microcircuit in mouse motor cortex.
AuthorsKiritani T, Wickersham IR, Seung HS, Shepherd GM,
JournalJ Neurosci
PubMed ID22492054
The generation of purposive movement by mammals involves coordinated activity in the corticospinal and corticostriatal systems, which are involved in different aspects of motor control. In the motor cortex, corticospinal and corticostriatal neurons are closely intermingled, raising the question of whether and how information flows intracortically within and across these ... More
The zebra finch paradox: song is little changed, but number of neurons doubles.
AuthorsWalton C, Pariser E, Nottebohm F,
JournalJ Neurosci
PubMed ID22262875
New neurons are added to the high vocal center (HVC) of adult males in seasonally breeding songbirds such as the canary (Serinus canaria) that learns new songs in adulthood, and the song sparrow (Melospiza melodia) that does not. In both cases, the new neurons numerically replace others that have died, ... More
NLRP3 inflammasomes are required for atherogenesis and activated by cholesterol crystals.
AuthorsDuewell P, Kono H, Rayner KJ, Sirois CM, Vladimer G, Bauernfeind FG, Abela GS, Franchi L, Nuñez G, Schnurr M, Espevik T, Lien E, Fitzgerald KA, Rock KL, Moore KJ, Wright SD, Hornung V, Latz E,
JournalNature
PubMed ID20428172
The inflammatory nature of atherosclerosis is well established but the agent(s) that incite inflammation in the artery wall remain largely unknown. Germ-free animals are susceptible to atherosclerosis, suggesting that endogenous substances initiate the inflammation. Mature atherosclerotic lesions contain macroscopic deposits of cholesterol crystals in the necrotic core, but their appearance ... More
Cytoskeletal control of CD36 diffusion promotes its receptor and signaling function.
The mechanisms that govern receptor coalescence into functional clusters--often a critical step in their stimulation by ligand--are poorly understood. We used single-molecule tracking to investigate the dynamics of CD36, a clustering-responsive receptor that mediates oxidized LDL uptake by macrophages. We found that CD36 motion in the membrane was spatially structured ... More
Specific RNA binding to ordered phospholipid bilayers.
AuthorsJanas T, Janas T, Yarus M,
JournalNucleic Acids Res
PubMed ID16641318
We have studied RNA binding to vesicles bounded by ordered and disordered phospholipid membranes. A positive correlation exists between bilayer order and RNA affinity. In particular, structure-dependent RNA binding appears for rafted (liquid-ordered) domains in sphingomyelin-cholesterol-1,2-dioleoyl-sn-glycero-3-phosphocholine vesicles. Binding to more highly ordered gel phase membranes is stronger, but much less ... More
A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale.
AuthorsBohland JW, Wu C, Barbas H, Bokil H, Bota M, Breiter HC, Cline HT, Doyle JC, Freed PJ, Greenspan RJ, Haber SN, Hawrylycz M, Herrera DG, Hilgetag CC, Huang ZJ, Jones A, Jones EG, Karten HJ, Kleinfeld D, Kötter R, Lester HA, Lin JM, Mensh BD, Mikula S, Panksepp J, Price JL, Safdieh J, Saper CB, Schiff ND, Schmahmann JD, Stillman BW, Svoboda K, Swanson LW, Toga AW, Van Essen DC, Watson JD, Mitra PP,
JournalPLoS Comput Biol
PubMed ID19325892
In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is critical, however, for both basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale ... More
Rac-mediated macropinocytosis is a critical route for naked plasmid DNA transfer in mice.
AuthorsFumoto S, Nishi J, Ishii H, Wang X, Miyamoto H, Yoshikawa N, Nakashima M, Nakamura J, Nishida K,
JournalMol Pharm
PubMed ID19492848
We have recently discovered the potential for in vivo naked plasmid DNA (pDNA) transfer into gastric serosal surface cells in mice. As pDNA are huge molecules, the mechanism of gene transfer without carriers and physical forces is of great biological interest. The endocytic route for naked pDNA transfer into gastric ... More
Ca2+-activated IK1 channels associate with lipid rafts upon cell swelling and mediate volume recovery.
AuthorsBarfod ET, Moore AL, Roe MW, Lidofsky SD
JournalJ Biol Chem
PubMed ID17264085
Restoration of cell volume in the continued presence of osmotic stimuli is essential, particularly in hepatocytes, which swell upon nutrient uptake. Responses to swelling involve the Ca2+-dependent activation of K+ channels, which promote fluid efflux to drive volume recovery; however, the channels involved in hepatocellular volume regulation have not been ... More
Glycosphingolipid-facilitated membrane insertion and internalization of cobra cardiotoxin. The sulfatide.cardiotoxin complex structure in a membrane-like environment suggests a lipid-dependent cell-penetrating mechanism for membrane binding polypeptides.
AuthorsWang CH, Liu JH, Lee SC, Hsiao CD, Wu WG
JournalJ Biol Chem
PubMed ID16263708
Cobra cardiotoxins, a family of basic polypeptides having lipid- and heparin-binding capacities similar to the cell-penetrating peptides, induce severe tissue necrosis and systolic heart arrest in snakebite victims. Whereas cardiotoxins are specifically retained on the cell surface via heparan sulfate-mediated processes, their lipid binding ability appears to be responsible, at ... More
Robust Myelination of Regenerated Axons Induced by Combined Manipulations of GPR17 and Microglia.
Authors
JournalNeuron
PubMed ID33108748
Sympathetic axonal sprouting induces changes in macrophage populations and protects against pancreatic cancer.
Authors
JournalNat Commun
PubMed ID35418199
The hippocampus converts dynamic entorhinal inputs into stable spatial maps.
Authors
JournalNeuron
PubMed ID34619088
Superior Colliculus to VTA pathway controls orienting response and influences social interaction in mice.
Authors
JournalNat Commun
PubMed ID35145124
Sensory Experience Engages Microglia to Shape Neural Connectivity through a Non-Phagocytic Mechanism.
Authors
JournalNeuron
PubMed ID32931754
Distinct Hippocampal Pathways Mediate Dissociable Roles of Context in Memory Retrieval.
Authors
JournalCell
PubMed ID27773481
CNS axonal degeneration and transport deficits at the optic nerve head precede structural and functional loss of retinal ganglion cells in a mouse model of glaucoma.
Authors
JournalMol Neurodegener
PubMed ID32854767
Serotonin engages an anxiety and fear-promoting circuit in the extended amygdala.
Authors
JournalNature
PubMed ID27556938
Postmitotic control of sensory area specification during neocortical development.
Authors
JournalNat Commun
PubMed ID25476200
Dynamic caveolae exclude bulk membrane proteins and are required for sorting of excess glycosphingolipids.