Cell-free reconstitution of vacuole membrane fragmentation reveals regulation of vacuole size and number by TORC1.
AuthorsMichaillat L, Baars TL, Mayer A,
JournalMol Biol Cell
PubMed ID22238359
'Size and copy number of organelles are influenced by an equilibrium of membrane fusion and fission. We studied this equilibrium on vacuoles-the lysosomes of yeast. Vacuole fusion can readily be reconstituted and quantified in vitro, but it had not been possible to study fission of the organelle in a similar ... More
Novel Fusogenic Liposomes for Fluorescent Cell Labeling and Membrane Modification.
AuthorsCsisza´r A, Hersch N, Dieluweit S, Biehl R, Merkel R, Hoffmann B,
JournalBioconjug Chem
PubMed ID20184308
'Efficient delivery of biomolecules into membranes of living cells as well as cell surface modifications are major biotechnological challenges. Here, novel liposome systems based on neutral and cationic lipids in combination with lipids modified by aromatic groups are introduced for such applications. The fusion efficiency of these liposome systems was ... More
Group V phospholipase A2 induces leukotriene biosynthesis in human neutrophils through the activation of group IVA phospholipase A2.
AuthorsKim YJ, Kim KP, Han SK, Munoz NM, Zhu X, Sano H, Leff AR, Cho W
JournalJ Biol Chem
PubMed ID12124392
'We reported previously that exogenously added human group V phospholipase A(2) (hVPLA(2)) could elicit leukotriene B(4) (LTB(4)) biosynthesis in human neutrophils (Han, S. K., Kim, K. P., Koduri, R., Bittova, L., Munoz, N. M., Leff, A. R., Wilton, D. C., Gelb, M. H., and Cho, W. (1999) J. Biol. Chem. ... More
Determination of the depth of BODIPY probes in model membranes by parallax analysis of fluorescence quenching.
AuthorsKaiser RD, London E
JournalBiochim Biophys Acta
PubMed ID9767081
'The location of a series of lipophilic and lipid-attached BODIPY (4, 4-difluoro-4-bora-3a,4a-diaza-s-indacene) membrane probes was analyzed by the quenching of BODIPY fluorescence by a series of nitroxide-labeled lipids in which the depth of the nitroxide group is varied. When attached to the polar headgroup of PE the BODIPY remained near ... More
The antimicrobial peptide trichogin and its interaction with phospholipid membranes.
AuthorsEpand RF, Epand RM, Monaco V, Stoia S, Formaggio F, Crisma M, Toniolo C
JournalEur J Biochem
PubMed ID10583397
'The interaction of the antimicrobial peptide trichogin GA IV with phospholipid bilayers has been studied. A series of analogs of trichogin was synthesized in which the nitroxide spin label, 4-amino-4-carboxy-2,2,6,6-tetramethylpiperidino-1-oxyl (TOAC), replaced one of the three alpha-aminoisobutyric acid (Aib) residues in the sequence. These modified peptides were used to assess ... More
Depth-profiling with giant vesicle membranes.
AuthorsMenger FM, Keiper JS, Caran KL
JournalJ Am Chem Soc
PubMed ID12358515
Labeling membranes with fluorescent phosphatidylethanolamine.
AuthorsChazotte B,
JournalCold Spring Harb Protoc
PubMed ID21536759
The phospholipid phosphatidylethanolamine (PE) can be conjugated via its head group to a number of fluorophores, including rhodamine, BODIPY (boron-dipyrromethene; 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene), and NBD (N-(7-nitrobenz-2-oxa-1,3-diazole-4-yl)). These probes can be used to label biological membranes and to study phospholipids within membranes. Rhodamine-PE, which does not readily exchange between lipid bilayers, has also ... More
A common pathway for the uptake of surfactant lipids by alveolar cells.
AuthorsPoelma DL, Ju MR, Bakker SC, Zimmermann LJ, Lachmann BF, van Iwaarden JF
JournalAm J Respir Cell Mol Biol
PubMed ID14644915
The uptake of different surfactant lipids-dipalmitoylphosphatidylcholine (DPPC), phosphatidylglycerol (PG), or phosphatidylinositol (PI)-and liposomes with a surfactant-like composition by alveolar type II cells (alveolar type II cells) and macrophages (alveolar macrophages) was studied in vitro. Fluorescent-labeled liposomes containing either 86% of the studied lipid, i.e., DPPC, PG, PI, and 6% labeled ... More
DNA release from lipoplexes by anionic lipids: correlation with lipid mesomorphism, interfacial curvature, and membrane fusion.
AuthorsTarahovsky YS, Koynova R, MacDonald RC
JournalBiophys J
PubMed ID15298910
DNA release from lipoplexes is an essential step during lipofection and is probably a result of charge neutralization by cellular anionic lipids. As a model system to test this possibility, fluorescence resonance energy transfer between DNA and lipid covalently labeled with Cy3 and BODIPY, respectively, was used to monitor the ... More
PD-L1:CD80 Cis-Heterodimer Triggers the Co-stimulatory Receptor CD28 While Repressing the Inhibitory PD-1 and CTLA-4 Pathways.