ELISA Assay Buffer (5X)
ELISA Assay Buffer (5X)
Invitrogen™

ELISA Assay Buffer (5X)

ELISA Assay Buffer (5X) is intended for use as: blocking reagent for ELISA plates, diluent for ELISA standards and samples,了解更多信息
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货号数量
DS98200200 mL
货号 DS98200
价格（CNY)
2,010.00
飞享价
Ends: 31-Dec-2025
2,675.00
共减 665.00 (25%)
Each
添加至购物车
数量:
200 mL
价格（CNY)
2,010.00
飞享价
Ends: 31-Dec-2025
2,675.00
共减 665.00 (25%)
Each
添加至购物车
ELISA Assay Buffer (5X) is intended for use as: blocking reagent for ELISA plates, diluent for ELISA standards and samples, diluent for the detection antibody, and diluent for the HRP conjugate. This buffer is recommended when using Invitrogen Antibody Pairs or when developing a sandwich ELISA.

For a complete kit of ELISA buffers, see ELISA Buffer Kit.
View all available buffers and reagents for ELISA applications.

For Research Use Only. Not for use in diagnostic procedures.
规格
运输条件Wet Ice
应用ELISA
形式Concentrated
数量200 mL
Unit SizeEach
内容与储存
Buffer. Store at 2-8°C.

常见问题解答 (FAQ)

How do I develop a sandwich ELISA using Cytosets?

Each CytoSets contains capture (coating) antibody, biotinlyated detection antibody, standard and Streptavidin-HRP. Other reagents required are listed in the CytoSets information sheet included with the kit and can be purshased from us separately (Antibody Pair Buffer kit CNB0011, 5x Assay Buffer DS98200, etc.). The information sheet also provides a specific procedure and illustrates an example standard curve which can be obtained when the specific procedure is followed. A general procedure is summarized here:

1) Coat the microplate with diluted capture (coating) antibody overnight at 2-8 degrees C; Wash the plate
2) Incubate standards or samples with the coated microplate; Wash the plate
3) Incubate diluted biotinlyated detection antibody with the plate; Wash the plate
4) Incubate Streptavidin-HRP with the plate for 15-45 minutes; Wash the plate
5) Incubate the plate with TMB substrate for 10-60 minutes and stop the reaction with Stop solution
6) Read microplate at 450 nm.

Investigators are advised to determine optimal buffer formulations, concentrations and incubation times for individual applications.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.