ER-Tracker™ Green (BODIPY™ FL Glibenclamide), for live-cell imaging
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ER-Tracker™ Green (BODIPY™ FL Glibenclamide), for live-cell imaging
Citations & References (28)
Invitrogen™

ER-Tracker™ Green (BODIPY™ FL Glibenclamide), for live-cell imaging

ER-Tracker Green dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER) that when stained usingRead more
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Catalog NumberQuantity
E34251100 μg
Catalog number E34251
Price (CNY)
4,692.00
Online Exclusive
Ends: 31-Dec-2025
6,359.00
Save 1,667.00 (26%)
Each
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Quantity:
100 μg
Price (CNY)
4,692.00
Online Exclusive
Ends: 31-Dec-2025
6,359.00
Save 1,667.00 (26%)
Each
Add to cart
ER-Tracker Green dye is cell-permeant, live-cell stain that is highly selective for the endoplasmic reticulum (ER) that when stained using the protocol provided, the staining pattern is partially retained after fixation with formaldehyde. This stain consists of the green-fluorescent BODIPY FL dye and glibenclamide. Glibenclamide (glyburide) binds to the sulphonylurea receptors of ATP-sensitive K+ channels which are prominent on ER; the pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell types may result in non-ER labeling.

Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorGreen
Detection MethodFluorescence
For Use With (Equipment)Fluorescence Microscope
Product LineBODIPY, ER-Tracker
Quantity100 μg
Shipping ConditionRoom Temperature
Label TypeBODIPY Dyes
Product TypeDye
SubCellular LocalizationEndoplasmic Reticulum
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

Why don't I see a significant change in signal for my live-cell fluorescent indicator dye?

Regardless of the type of live-cell indicator dye (e.g., calcium indicators, pH indicator, metal ion indicators), make sure there is no serum during the loading step, which can prematurely cleave dyes with AM esters and bind dyes non-specifically. Always optimize the dye concentration and staining time with a positive control before you run your test samples, to give the best signal-to-background. Always run a positive control with a buffer containing free ions of known concentration and an ionophore to open pores to those ions (for instance, for calcium indicators like Fluo-4 AM, this would include a buffer with added calcium combined with calcimycin, or for pH indicators, buffers of different pHs combined with nigericin). Reactive oxygen indicators, such as CellROX Green or H2DCFDA would require a cellular reactive oxygen species (ROS) stimulant as a positive control, such as menadione. Finally, make sure your imaging system has a sensitive detector. Plate readers, for instance, have much lower detector efficiency over background, compared to microscopy or flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (28)

Citations & References
Abstract
Morphological localisation of sulfonylurea receptor 1 in endocrine cells of human, mouse and rat pancreas.
Authors:Guiot Y, Stevens M, Marhfour I, Stiernet P, Mikhailov M, Ashcroft SJ, Rahier J, Henquin JC, Sempoux C,
Journal:Diabetologia
PubMed ID:17593344
'AIMS/HYPOTHESIS: Sulfonylurea receptor 1 (SUR1) is the regulatory subunit of ATP-sensitive K channels in beta cells. Morphological methods (immunohistochemistry and sulfonylurea binding) were used to establish the cellular and subcellular location of SUR1 in human and rodent islets. RESULTS: In the human, mouse and rat pancreas, all endocrine cells of ... More
Live intracellular super-resolution imaging using site-specific stains.
Authors:Carlini L, Manley S,
Journal:
PubMed ID:24079385
'Point localization super-resolution imaging (SR) requires dyes that can cycle between fluorescent and dark states, in order for their molecular positions to be localized and create a reconstructed image. Dyes should also densely decorate biological features of interest to fully reveal structures being imaged. We tested site-specific dyes in several ... More
Suicidal membrane repair regulates phosphatidylserine externalization during apoptosis.
Authors:Mirnikjoo B, Balasubramanian K, Schroit AJ,
Journal:J Biol Chem
PubMed ID:19561081
One of the hallmarks of apoptosis is the redistribution of phosphatidylserine (PS) from the inner-to-outer plasma membrane (PM) leaflet, where it functions as a ligand for phagocyte recognition and the suppression of inflammatory responses. The mechanism by which apoptotic cells externalize PS has been assumed to involve  ... More
Mitochondrial autophagy is an HIF-1-dependent adaptive metabolic response to hypoxia.
Authors:Zhang H, Bosch-Marce M, Shimoda LA, Tan YS, Baek JH, Wesley JB, Gonzalez FJ, Semenza GL,
Journal:J Biol Chem
PubMed ID:18281291
Autophagy is a process by which cytoplasmic organelles can be catabolized either to remove defective structures or as a means of providing macromolecules for energy generation under conditions of nutrient starvation. In this study we demonstrate that mitochondrial autophagy is induced by hypoxia, that this process requires the hypoxia-dependent factor-1-dependent ... More
Translocation of a phycoerythrin alpha subunit across five biological membranes.
Authors:Gould SB, Fan E, Hempel F, Maier UG, Klösgen RB,
Journal:J Biol Chem
PubMed ID:17702756
Cryptophytes, unicellular algae, evolved by secondary endosymbiosis and contain plastids surrounded by four membranes. In contrast to cyanobacteria and red algae, their phycobiliproteins do not assemble into phycobilisomes and are located within the thylakoid lumen instead of the stroma. We identified two gene families encoding phycoerythrin alpha and light-harvesting complex ... More
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