PD-L1 小鼠 ProcartaPlex™ Simplex 试剂盒
PD-L1 小鼠 ProcartaPlex™ Simplex 试剂盒
Invitrogen™

PD-L1 小鼠 ProcartaPlex™ Simplex 试剂盒

小鼠 PD-L1 Simplex ProcartaPlex 试剂盒可以测量 PD-L1 蛋白并与其他 Simplex 试剂盒组合使用,这样,您可以创建自己的利用 Luminex了解更多信息
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货号数量
EPX010-26054-90196 次测试
货号 EPX010-26054-901
价格(CNY)
-
数量:
96 次测试
小鼠 PD-L1 Simplex ProcartaPlex 试剂盒可以测量 PD-L1 蛋白并与其他 Simplex 试剂盒组合使用,这样,您可以创建自己的利用 Luminex xMAP 技术进行蛋白检测/定量的多重检测组合。当多个 Simplex 试剂盒结合使用时(即,当您未使用预配置的多通路检测组合时),每个检测板仅需要一个缓冲液试剂盒(单独出售),无需考虑通路大小。

关于用于 Luminex 平台的 ProcartaPlex 检测

ProcartaPlex 免疫检测基于夹心式 ELISA 的原理,使用与一种蛋白的不同抗原决定簇结合的两种高度特异性抗体,通过 Luminex 仪器同时定量所有蛋白靶标。ProcartaPlex 多通路检测只需 25 µL 血浆或血清,或 50 µL 细胞培养物上清液,只需四小时即可获得分析结果。

每份样品可得到更多结果—在单一 25 - 50 µL 样品中可同时测量多种蛋白靶标

成熟的 Luminex 技术—较常参考的蛋白检测和定量测定多通路平台

ProcartaPlex 检测利用 Luminex xMAP(多分析物分析)技术用于在单一 25-50 µL 样品(血浆、血清、细胞培养物上清液和其他体液)中同时检测和定量测定多达 80 种蛋白靶标。

ProcartaPlex 检测中的 Luminex 微珠用精确比例的红色和红外荧光基团进行内部染色,生成光谱独特的标记,可通过 Luminex xMAP 检测系统(例如 Luminex 200、FLEXMAP 3D 和 MAGPIX)识别。与夹心ELISA类似,ProcartaPlex测定使用匹配的抗体对来鉴定目的蛋白质。在多通路检测中,每种光谱独特的微珠用单一靶蛋白特异性抗体标记,并且用生物素化抗体和链霉素亲和素 R-藻红蛋白 (RPE) 识别结合蛋白。蛋白特异性抗体与独特微珠偶联可用于在单孔中分析多个靶标。

ProcartaPlex 检测与 ELISA 之间极显著的区别在于 ProcartaPlex 检测中的捕获抗体与微珠偶联并且不吸附于微孔板孔中,因此 ProcartaPlex 检测试剂在溶液中自由漂浮。例如,对于检测,Luminex 200仪器包含两个激光器,一个用于区分每个珠子的光谱特征,另一个用于量化RPE荧光的量,其与样品中存在的蛋白质的量成比例。ProcartaPlex 多通路测定可以在样品量明显减少的情况下分析更多靶蛋白,同时还能执行传统三明治 ELISA。

ProcartaPlex 多通路检测组合有多种规格,可以覆盖六个物种(人、小鼠、大鼠、非人灵长类动物、猪和犬)。
仅供科研使用。不可用于诊断程序。
规格
测定范围根据批次 1 测定:26至 107600 pg/mL
检测灵敏度根据批次 1 测定:3.1 pg/mL
磁珠类型PD-L1 [29 ]
适用于(设备)Luminex™ 仪器
产品规格Simplex 试剂盒
基因CD274 抗原
基因别名B7h1,Pdcd1l1,Pdcd1lg1,Pdl1
基因 ID (Entrez)60533
基因符号CD274
产品线ProcartaPlex™
蛋白质程序性细胞死亡 1 配体 1
蛋白质子类型B7-H1
样品类型血清、血浆、细胞培养物上清液, Plasma, Cell Culture Supernatants
样品体积血清,血浆:25 μL;CCS:50 μL
运输条件湿冰
UniProt IDQ9EP73
可组合性可结合使用
产品类型Simplex 试剂盒
数量96 次测试
研究领域Immunology, Oncology
种属小鼠
Unit SizeEach
内容与储存
  • 1 小瓶捕获微珠 (50X)
  • 1 小瓶生物素化检测抗体 (50X)
  • 2 小瓶小鼠标准品混合物 F(冻干)
  • 存储在 2°C 到 8°C。

常见问题解答 (FAQ)

Do you offer a Mac-compatible version of the ProcartaPlex Analyst Software?

Unfortunately, we do not currently offer a Mac-compatible version of the ProcartaPlex Analyst Software. ProcartaPlex Analyst Software is compatible with PC, or xPONENT Software is available on Luminex instruments to analyze your data.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do you offer FlowCytomix assays?

Sorry, the FlowCytomix product line has been discontinued. However, we offer a new antibody and magnetic bead-based detection system, i.e., ProcartaPlex assays for multiplex protein quantitation using the Luminex instrument platform.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How long does it take to acquire ProcartaPlex assay data on the different Luminex instruments?

With everything working smoothly, here is the approximate read time for one plate on different Luminex instruments:

- FLEXMAP 3D Systems and INTELLIFLEX Systems will read 96 well plates in ~20 min, and 384 well plates in ~75 min.
- Luminex 100/200 Systems will read 96 well plates in ~40 min.
- MAGPIX System will read 96 well plates in ~50-60 min. Please note that these are estimates for the read times of the plates only, and do not include time for calibration or set-up of the instrument.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is the sensitivity value for a Multiplex ProcartaPlex assay different when compared to the sensitivity for those same analytes assayed as Simplex assays?

In general, sensitivity between Simplex and Multiplex ProcartaPlex assay kits is comparable.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Why does the ProcartaPlex ULOQ (Upper Limit of Quantitation) and LLOQ (Lower Limit of Quantitation) change between different lots of standards?

We supply the standard protein in a lyophilized form. Lyophilization is a dehydration process that preserves the perishable standard. We have demonstrated that often, the amount of starting protein prior to lyophilization and that of the final product after lyophilization varies (this could be different from analyte to analyte). To ensure that we deliver the most quantitative and reproducible product, we always calibrate the standard after lyophilization and therefore, the concentration range can vary from lot to lot of the standard.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.