MMP 3-Plex Human ProcartaPlex™ 检测组合 2
Invitrogen™

MMP 3-Plex Human ProcartaPlex™ 检测组合 2

人 MMP 3-Plex ProcartaPlex 检测组合 2 能够使研究人员通过分析以下 3 种蛋白靶标来探索炎症和组织修复:MMP-2、MMP-3 和 MMP-9,使用 Luminex xMAP 技术进行。
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货号数量
EPX03A-10829-90196 次测试
货号 EPX03A-10829-901
价格(CNY)
-
数量:
96 次测试

人 MMP 3-Plex ProcartaPlex 检测组合 2 能够使研究人员通过分析以下 3 种蛋白靶标来探索炎症和组织修复:MMP-2、MMP-3 和 MMP-9,使用 Luminex xMAP 技术进行。同时检测这些靶标有助于探索生理过程,如生殖或任何其他良性或恶性组织重构过程。

对 ProcartaPlex 预配置检测组合进行了广泛的分析物结合、干扰和交叉反应性检测,以提供较高水平的验证和精密度。所有 ProcartaPlex 检测组合均配有进行检测所需的试剂。
有关更多信息和可用产品,请参见 ProcartaPlex 免疫检测页面。

靶标列表 [微珠区域]:MMP-2 [18]、MMP-3 [19]、MMP-9 [20]

推荐的样本预稀释度:血清,血浆:1:100

关于用于 Luminex 平台的 ProcartaPlex 检测
ProcartaPlex 免疫检测基于夹心式 ELISA 的原理,使用与一种蛋白的不同抗原决定簇结合的两种高度特异性抗体,通过 Luminex 仪器同时定量所有蛋白靶标。ProcartaPlex 多通路检测只需 25 μL 血浆或血清,或 50 μL 细胞培养物上清液,只需四小时即可获得分析结果。

其特性包括:
• 可重现的可靠结果—经验证检测组合符合较高行业标准,包括蛋白靶标可结合性和交叉反应性检测
• 每个样品可得到更多结果—在单个 25–50 μL 样品中同时测量多种蛋白靶标
• 成熟的 Luminex 技术 — 经常被引用的蛋白检测和定量多通路平台

ProcartaPlex 检测利用 Luminex xMAP(多分析物分析)技术在单个 25–50 μL 样品(血浆、血清、细胞培养上清液和其他体液)中同时检测和定量多达 80 种蛋白靶标。

ProcartaPlex 检测中的 Luminex 微珠用精确比例的红色和红外荧光基团进行内部染色,生成光谱独特的标记,可通过 Luminex xMAP 检测系统(如 Luminex 200、FLEXMAP 3D 和 MAGPIX)识别。与夹心ELISA类似,ProcartaPlex测定使用匹配的抗体对来鉴定目的蛋白质。在多通路测定中,每种光谱独特的微珠用单一靶蛋白特异性抗体标记,并且用生物素化抗体和链霉素亲和素–R-藻红蛋白 (RPE) 识别结合的蛋白。蛋白特异性抗体与独特微珠偶联可用于在单孔中分析多个靶标。

ProcartaPlex 检测与 ELISA 之间极显著的区别在于 ProcartaPlex 检测中的捕获抗体与微珠偶联并且不吸附于微孔板孔中,因此 ProcartaPlex 检测试剂在溶液中自由漂浮。例如,对于检测,Luminex 200仪器包含两个激光器,一个用于区分每个珠子的光谱特征,另一个用于量化RPE荧光的量,其与样品中存在的蛋白质的量成比例。ProcartaPlex 多通路测定可以在样品量明显减少的情况下分析更多靶蛋白,同时还能执行传统三明治 ELISA。

仅供科研使用。不可用于诊断程序。
规格
测定范围参见检验报告
检测灵敏度参见检验报告
检测方法荧光
适用于(设备)Luminex™ 仪器
产品规格多通路试剂盒
产品线ProcartaPlex
样品类型血清、血浆、细胞培养物上清液, Plasma, Cell Culture Supernatants
样品体积血清,血浆:25 μL;CCS:50 μL
运输条件湿冰
可组合性不可结合使用
产品类型多通路检测组合
数量96 次测试
研究领域Immunology
种属
Unit SizeEach
内容与储存
  • 2 小瓶人标准品混合物 MMP(冻干)
  • 1 小瓶捕获微珠混合物 (1 X)
  • 1 小瓶生物素化检测抗体混合物 (50 X)
  • 1 瓶检测抗体稀释液 (1 X)
  • 1 瓶读取缓冲液 (1X)
  • 1 瓶链霉素亲和素-PE (1 X)
  • 1 瓶 Universal Assay Buffer (10 x)
  • 1 瓶洗涤缓冲液 (10 x)
  • 8 联排管
  • 黏性封板膜
  • 平底 96 孔板,黑色
  • 储存于 2°C 至 8°C

常见问题解答 (FAQ)

Do you offer a Mac-compatible version of the ProcartaPlex Analyst Software?

Unfortunately, we do not currently offer a Mac-compatible version of the ProcartaPlex Analyst Software. ProcartaPlex Analyst Software is compatible with PC, or xPONENT Software is available on Luminex instruments to analyze your data.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do you offer FlowCytomix assays?

Sorry, the FlowCytomix product line has been discontinued. However, we offer a new antibody and magnetic bead-based detection system, i.e., ProcartaPlex assays for multiplex protein quantitation using the Luminex instrument platform.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How long does it take to acquire ProcartaPlex assay data on the different Luminex instruments?

With everything working smoothly, here is the approximate read time for one plate on different Luminex instruments:

- FLEXMAP 3D Systems and INTELLIFLEX Systems will read 96 well plates in ~20 min, and 384 well plates in ~75 min.
- Luminex 100/200 Systems will read 96 well plates in ~40 min.
- MAGPIX System will read 96 well plates in ~50-60 min. Please note that these are estimates for the read times of the plates only, and do not include time for calibration or set-up of the instrument.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is the sensitivity value for a Multiplex ProcartaPlex assay different when compared to the sensitivity for those same analytes assayed as Simplex assays?

In general, sensitivity between Simplex and Multiplex ProcartaPlex assay kits is comparable.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Why does the ProcartaPlex ULOQ (Upper Limit of Quantitation) and LLOQ (Lower Limit of Quantitation) change between different lots of standards?

We supply the standard protein in a lyophilized form. Lyophilization is a dehydration process that preserves the perishable standard. We have demonstrated that often, the amount of starting protein prior to lyophilization and that of the final product after lyophilization varies (this could be different from analyte to analyte). To ensure that we deliver the most quantitative and reproducible product, we always calibrate the standard after lyophilization and therefore, the concentration range can vary from lot to lot of the standard.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

引用和文献 (2)

引用和文献
Abstract
On the prospect of serum exosomal miRNA profiling and protein biomarkers for the diagnosis of ascending aortic dilatation in patients with bicuspid and tricuspid aortic valve.
Authors:Gallo A, Agnese V, Coronnello C, Raffa GM, Bellavia D, Conaldi PG, Pilato M, Pasta S
Journal:Int J Cardiol
PubMed ID:30297190
To determine the impact of circulating miRNA and protein activity on the severity of ascending aortic dilatation in patients with bicuspid (BAV) and tricuspid aortic valve (TAV). ... More
Effect of omalizumab on bronchoalveolar lavage matrix metalloproteinases in severe allergic asthma.
Authors:Zastrzezynska W, Bazan-Socha S, Przybyszowski M, Gawlewicz-Mroczka A, Jakiela B, Plutecka H, Zareba L, Musial J, Okon K, Sladek K, Soja J
Journal:J Asthma
PubMed ID:33764254
Airway inflammation in asthma is accompanied by reconstruction of the bronchial wall extracellular matrix that most likely occurs with a contribution of matrix metalloproteinases (MMPs). Recently we have reported that omalizumab may decrease reticular basement membrane (RBM) thickness together with fibronectin deposits in asthmatic airways, although mechanisms involved are unknown. ... More