Image-iT™ 红色缺氧监测试剂
Image-iT™ 红色缺氧监测试剂
Invitrogen™

Image-iT™ 红色缺氧监测试剂

Image-iT™ 红色缺氧监测试剂是一种新型的荧光化合物,用于测定活细胞中的缺氧情况。活细胞在具有正常氧气浓度的环境中不发出荧光,而当氧水平降低时会发出荧光。Image-iT™ 红色缺氧监测试剂是一种实时氧检测器,当细胞恢复至正常氧水平时,荧光响应会逆转。Image-iT™ 红色缺氧监测试剂的特点包括:•在低氧环境中发出荧光可以测量活细胞缺氧• 实时氧检测器,具有可逆的荧光响应• 易于使用—只需添加到细胞培养基中并成像Image-iT™ 红色缺氧监测试剂是一种在低氧浓度环境中发出荧光的荧光化合物,并且它可渗透活细胞了解更多信息
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货号数量
H104981 mg
货号 H10498
价格(CNY)
6,804.00
Each
添加至购物车
数量:
1 mg
价格(CNY)
6,804.00
Each
添加至购物车
Image-iT™ 红色缺氧监测试剂是一种新型的荧光化合物,用于测定活细胞中的缺氧情况。活细胞在具有正常氧气浓度的环境中不发出荧光,而当氧水平降低时会发出荧光。Image-iT™ 红色缺氧监测试剂是一种实时氧检测器,当细胞恢复至正常氧水平时,荧光响应会逆转。

Image-iT™ 红色缺氧监测试剂的特点包括:
•在低氧环境中发出荧光可以测量活细胞缺氧
• 实时氧检测器,具有可逆的荧光响应
• 易于使用—只需添加到细胞培养基中并成像

Image-iT™ 红色缺氧监测试剂是一种在低氧浓度环境中发出荧光的荧光化合物,并且它可渗透活细胞。这些特性使其成为在缺氧条件中检测细胞和组织的非常有用的工具。Image-iT™ 红色缺氧监测试剂是一种非常灵敏的氧检测器。与仅对极低氧水平产生反应的哌莫硝唑加合物不同,当大气氧水平低于 5% 时,Image-iT™ 缺氧监测试剂开始发出荧光。它可快速响应这种环境,并且如果氧浓度升高,荧光响应会逆转。这些特性使 Image-iT™ 红色缺氧监测试剂成为检测肿瘤、3D 培养物、球体、神经元等周围缺氧条件的理想工具。它可以用于检测小动物中的肿瘤,其荧光特性已显示出与缺氧环境中的 Hif 1α 表达和转移的增加相对应 (1)。

1.Zhang, S., Hosaka, M., Yoshihara, T., Negishi, K., Iida, Y., Tobita, S., & Takeuchi, T. (2010) 磷光–发射铱复合物作为一个缺氧感应探头,用于活体动物中的肿瘤成像。Cancer Research 70(11), 4490-4498.
规格
检测方法荧光
形式实心
数量1 mg
试剂类型氧气传感器
研究类别癌症研究
运输条件经批准可在室温下或置于湿冰上运输
产品线Image-iT™
产品类型荧光试剂
Unit SizeEach
内容与储存
以 1 mg 冻干固体形式提供。足够用于 3000 次测定(0.1 mL/测定)或 600 次测定(0.5 mL/测定)。
储存在 ≤-20°C 条件下时,产品在接收后可稳定保存至少 6 个月。
避光干燥储存。

常见问题解答 (FAQ)

Can Image-iT Red Hypoxia Reagent be used in cells expressing fluorescent proteins?

Yes, as long as the fluorescent protein does not emit in the red range (˜610 nm). For cells that do not express a fluorescent protein, we recommend viewing unstained cells under the TRITC and Texas Red channels to examine autofluorescence. For cells expressing a fluorescent protein, we recommend analyzing unstained cell samples to determine the extent the fluorescent protein emission may overlap in these channels.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

May I include a total cell stain (to stain all cells) along with Image-iT Hypoxia Reagent?

You may use cell-permeable nuclear counterstains or surface labels, but avoid any general cytoplasmic stains that may interfere with the emission of the hypoxia reagents.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

May I include a dead cell stain along with Image-iT Hypoxia Reagent?

Yes. We recommend using NucGreen Dead 488 ReadyProbes Reagent (Cat No. R37109) with Image-iT Red Hypoxia Reagent and NucRed Dead 647 ReadyProbes Reagent (Cat No. R37113) with Image-iT Green Hypoxia Reagent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Which filter set can I use for optimal excitation and emission with Image-iT Red Hypoxia Reagent?

The Image-iT Red Hypoxia Reagent has an approximate Ex/Em maxima at 490/610 nm. You may use a 488 nm laser and a Texas Red emission filter. For lamp-based instruments, a YFP longpass filter set may be used (excitation range from 485 to 510 nm and emission from 550 nm up to 620 nm).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.