Common intra-articular T cell expansions in patients with reactive arthritis: identical beta-chain junctional sequences and cytotoxicity toward HLA-B27.
JournalJournal of immunology (Baltimore, Md. : 1950)
PubMed ID10201900
Characterization and channel coupling of the P2Y(12) nucleotide receptor of brain capillary endothelial cells.
AuthorsSimon Joseph; Filippov Alexander K; Göransson Sara; Wong Yung H; Frelin Christian; Michel Anton D; Brown David A; Barnard Eric A;
JournalJ Biol Chem
PubMed ID12080041
Rat brain capillary endothelial (B10) cells express an unidentified nucleotide receptor linked to adenylyl cyclase inhibition. We show that this receptor in B10 cells is identical in sequence to the P2Y(12) ADP receptor ( ... More
Cercarial Elastase Is Encoded by a Functionally Conserved Gene Family across Multiple Species of Schistosomes.
AuthorsSalter Jason P.; Choe Youngchool; Albrecht Hugo; Franklin Christopher; Lim Kee-Chong; Craik Charles S.; McKerrow James H.;
JournalJ Biol Chem
PubMed ID11986325
Water borne cercaria(ae) of the trematode genus Schistosoma rapidly penetrate host skin. A single serine protease activity, cercarial elastase, is deposited in advance of the invading parasite by holocytosis of vesicles from ten large acetabular gland cells. Cercarial elastase activity is a composite of multiple isoforms. Genes coding for the ... More
RasGRP4, a new mast cell-restricted Ras guanine nucleotide-releasing protein with calcium- and diacylglycerol-binding motifs. Identification of defective variants of this signaling protein in asthma, mastocytosis, and mast cell leukemia patients and demonstration of the importance of RasGRP4 in mast cell development and function.
Authors Yang Yi; Li Lixin; Wong Guang W; Krilis Steven A; Madhusudhan M S; Sali Andrej; Stevens Richard L;
JournalJ Biol Chem
PubMed ID11956218
'A cDNA was isolated from interleukin 3-developed, mouse bone marrow-derived mast cells (MCs) that contained an insert (designated mRasGRP4) that had not been identified in any species at the gene, mRNA, or protein level. By using a homology-based cloning approach, the approximately 2.6-kb hRasGRP4 transcript was also isolated from the ... More
Novel Cav2.1 splice variants isolated from Purkinje cells do not generate P-type Ca2+ current.
'The alpha(1)2.1 (alpha(1A)) subunits of P-type and Q-type Ca(2+) channels are encoded by a single gene, Cacna1a. Although these channels differ in the inactivation kinetics and sensitivity to omega-agatoxin IVA, the mechanism underlying these differences remains to be clarified. Alternative splicings of the Cacna1a transcript have been postulated to contribute ... More
Role of trehalose phosphate synthase in anoxia tolerance and development in Drosophila melanogaster.
Authors Chen Qiaofang; Ma Enbo; Behar Kevin L; Xu Tian; Haddad Gabriel G;
JournalJ Biol Chem
PubMed ID11719513
'Recent studies have shown that trehalose plays a protective role in yeast in a variety of stresses, including heat, freezing and thawing, dehydration, hyperosmotic shock, and oxidant injury. Because (a) heat shock and anoxia share mechanisms that allow organisms to survive, (b) Drosophila melanogaster is tolerant to anoxia, and (c) ... More
Cloning and characterization of a cell surface receptor for xenotropic and polytropic murine leukemia viruses [see comments]
AuthorsTailor CS, Nouri A, Lee CG, Kozak C, Kabat D
JournalProc Natl Acad Sci U S A
PubMed ID9927670
'Xenotropic and polytropic murine leukemia viruses (X-MLVs and P-MLVs) cross-interfere to various extents in non-mouse species and in wild Asian mice, suggesting that they might use a common receptor for infection. Consistent with this hypothesis, the susceptibility of some wild mice to X-MLVs has been mapped to the P-MLV receptor ... More
The identification and functional characterization of a novel mast cell isoform of the microphthalmia-associated transcription factor.
AuthorsTakemoto CM, Yoon YJ, Fisher DE.
JournalJ Biol Chem
PubMed ID12039954
'The microphthalmia-associated transcription factor (Mitf) is critical for mast cell development based on the severe mast cell deficiency seen in Mitf mutant mice. Mitf also is important for the development of melanocytes, osteoclasts, and retinal pigment epithelium. The lineage-restricted phenotypes of Mitf mutations correlate with tissue-restricted expression of Mitf, a ... More
An imprinted PEG1/MEST antisense expressed predominantly in human testis and in mature spermatozoa.
Authors Li Tao; Vu Thanh H; Lee Kok-Onn; Yang Youwen; Nguyen Chuyen V; Bui Huy Q; Zeng Zhi-Lan; Nguyen Binh T; Hu Ji-Fan; Murphy Susan K; Jirtle Randy L; Hoffman Andrew R;
JournalJ Biol Chem
PubMed ID11821432
'PEG1 (or MEST) is an imprinted gene located on human chromosome 7q32 that is expressed predominantly from the paternal allele. In the mouse, Peg1/Mest is associated with embryonic growth and maternal behavior. Human PEG1 is transcribed from two promoters; the transcript from promoter P1 is derived from both parental alleles, ... More
Mutation of the matrix metalloproteinase At2-MMP inhibits growth and causes late flowering and early senescence in Arabidopsis.
Authors Golldack Dortje; Popova Olga V; Dietz Karl-Josef;
JournalJ Biol Chem
PubMed ID11726650
'This study characterizes the expression and functional significance of the member of the matrix metalloproteinase (MMP) family At2-MMP from Arabidopsis. By transcript analysis, expression of At2-MMP was found in leaves and roots of juvenile Arabidopsis and leaves, roots, and inflorescences of mature flowering plants showing strong increase of transcript abundance ... More
Induction of MUC2 and MUC5AC mucins by factors of the epidermal growth factor (EGF) family is mediated by EGF receptor/Ras/Raf/extracellular signal-regulated kinase cascade and Sp1.
'The 11p15 mucin genes (MUC2, MUC5AC, MUC5B and MUC6) possess a cell-specific pattern of expression in normal lung that is altered during carcinogenesis. Growth factors of the epidermal growth factor family are known to target key genes that in turn may affect the homeostasis of lung mucosae. Our aim was ... More
Functions of Transforming Growth Factor-beta Family Type I Receptors and Smad Proteins in the Hypertrophic Maturation and Osteoblastic Differentiation of Chondrocytes.
'We investigated the effects of bone morphogenetic protein (BMP)-2, a member of the transforming growth factor-beta superfamily, on the regulation of the chondrocyte phenotype, and we identified signaling molecules involved in this regulation. BMP-2 triggers three concomitant responses in mouse primary chondrocytes and chondrocytic MC615 cells. First, BMP-2 stimulates expression ... More
Secretory leukocyte protease inhibitor mediates proliferation of human endometrial epithelial cells by positive and negative regulation of growth-associated genes.
'Secretory leukocyte protease inhibitor (SLPI) inhibits chymotrypsin, trypsin, elastase, and cathepsin G. This protein also exhibits proliferative effects, although little is known about the molecular mechanisms underlying this activity. We have generated SLPI-ablated epithelial sublines by stably transfecting the Ishikawa human endometrial cell line with an antisense human SLPI RNA ... More
Plasmodium falciparum subtilisin-like protease 2, a merozoite candidate for the merozoite surface protein 1-42 maturase.
AuthorsBarale JC, Blisnick T, Fujioka H, Alzari PM, Aikawa M, Braun-Breton C, Langsley G
JournalProc Natl Acad Sci U S A
PubMed ID10339607
'The process of human erythrocyte invasion by Plasmodium falciparum parasites involves a calcium-dependent serine protease with properties consistent with a subtilisin-like activity. This enzyme achieves the last crucial maturation step of merozoite surface protein 1 (MSP1) necessary for parasite entry into the host erythrocyte. In eukaryotic cells, such processing steps ... More
Alternative Splicing of the Adenylyl Cyclase Stimulatory G-protein Galpha s Is Regulated by SF2/ASF and Heterogeneous Nuclear Ribonucleoprotein A1 (hnRNPA1) and Involves the Use of an Unusual TG 3'-Splice Site.
Authors Pollard Alison J; Krainer Adrian R; Robson Stephen C; Europe-Finner G Nicholas;
JournalJ Biol Chem
PubMed ID11825891
'The factors involved in regulating alternative splicing of the human adenylyl cyclase stimulatory G-protein Galpha(s) in different cell types remain undefined. We have designed a Galpha(s) minigene that retains the signals required for Galpha(s) alternative splicing in vivo. Employing transient transfection of human myometrial smooth muscle cells and HeLa cells, ... More
CSN1 N-terminal-dependent activity is required for Arabidopsis development but not for Rub1/Nedd8 deconjugation of cullins: a structure-function study of CSN1 subunit of COP9 signalosome.
Authors Wang Xiping; Kang Dingming; Feng Suhua; Serino Giovanna; Schwechheimer Claus; Wei Ning;
JournalMol Biol Cell
PubMed ID11854419
'The COP9 signalosome (CSN) is a multifunctional protein complex essential for arabidopsis development. One of its functions is to promote Rub1/Nedd8 deconjugation from the cullin subunit of the Skp1-cullin-F-box ubiquitin ligase. Little is known about the specific role of its eight subunits in deneddylation or any of the physiological functions ... More
CmC(A/T)GG DNA methylation in mature B cell lymphoma gene silencing.
Authors Malone C S; Miner M D; Doerr J R; Jackson J P; Jacobsen S E; Wall R; Teitell M;
JournalProc Natl Acad Sci U S A
PubMed ID11526227
'DNA methylation has been linked to gene silencing in cancer. Primary effusion lymphoma (PEL) and myeloma are lymphoid malignancies that arise from terminally differentiated B cells. Interestingly, PEL do not express immunoglobulins or most B lineage-specific genes. The B cell-specific B29 (Igbeta/CD79b) gene is silenced in PEL and some myelomas ... More
Interaction between Erbin and a Catenin-related protein in epithelial cells.
'Integrity of epithelial tissues relies on the proper apical-basolateral polarity of epithelial cells. Members of the LAP (LRR and PDZ) protein family such as LET-413 and Scribble are involved in maintaining epithelial cell polarity in Caenorhabditis elegans and Drosophila melanogaster, respectively. We previously described Erbin as a mammalian LET-413 homologue ... More
Nongenomic testosterone calcium signaling. Genotropic actions in androgen receptor-free macrophages.
AuthorsGuo Z, Benten WP, Krucken J, Wunderlich F
JournalJ Biol Chem
PubMed ID12048191
'Steroid hormones exert genotropic actions through members of the nuclear receptor family. Here, we have demonstrated genotropic actions of testosterone that are independent of intracellular androgen receptors (iAR). Through plasma membrane androgen receptors (mAR), testosterone induces a rapid rise in the intracellular free Ca(2+) concentration of iAR-free murine RAW 264.7 ... More
pp56Lck mediates TCR zeta-chain binding to themicrofilament cytoskeleton.
AuthorsRozdzial MM, Pleiman CM, Cambier JC, Finkel TH
JournalJ Immunol
PubMed ID9820525
'The TCR zeta-chain (zeta) on mature murine T lymphocytes binds to the microfilament cytoskeleton inresponse to Ag receptor ligation. Here, we report the role of Src family kinases in zeta-cytoskeletalbinding, using mutant mice and a cell-free model system. Binding of zeta to actin in the cell-free systemhas a specific requirement ... More
A Mouse Gene Encoding an Oocyte Antigen Associated with Autoimmune Premature Ovarian Failure
AuthorsZhi-Bin Tong and Lawrence M. Nelson
JournalEndocrinology
PubMed ID10433232
'Autoimmune premature ovarian failure causes young women to develop menopausal symptoms and infertility. A similar syndrome appears in mice with postthymectomy autoimmune premature ovarian failure. We demonstrate that these mice develop antibodies against a 125-kDa protein located in the oocyte cytoplasm (ooplasm). By screening a mouse ovarian complementary DNA expression ... More
The biosynthesis of the aromatic myxobacterial electron transport inhibitor stigmatellin is directed by a novel type of modular polyketide synthase.
'Deductions from the molecular analysis of the 65,000-bp stigmatellin biosynthetic gene cluster are reported. The biosynthetic genes (stiA-J) encode an unusual bacterial modular type I polyketide synthase (PKS) responsible for the formation of this aromatic electron transport inhibitor produced by the myxobacterium Stigmatella aurantiaca. Involvement of the PKS gene cluster ... More
Evidence of hybridity in invasive watermilfoil (Myriophyllum) populations.
Authors Moody Michael L; Les Donald H;
JournalProc Natl Acad Sci U S A
PubMed ID12407174
'Invasions of nonindigenous species have caused ecological devastation to natural communities worldwide, yet the biological bases for invasiveness remain poorly understood. Our studies of invasive watermilfoil (Myriophyllum) populations revealed widespread polymorphisms in biparentally inherited nuclear ribosomal DNA sequences, which were not detected in populations of native North American species. Subclones ... More
Investigating stem cells in human colon by using methylation patterns.
Authors Yatabe Y; Tavare S; Shibata D;
JournalProc Natl Acad Sci U S A
PubMed ID11553798
'The stem cells that maintain human colon crypts are poorly characterized. To better determine stem cell numbers and how they divide, epigenetic patterns were used as cell fate markers. Methylation exhibits somatic inheritance and random changes that potentially record lifelong stem cell division histories as binary strings or tags in ... More
Oncogenic potential of mouse translation elongation factor-1 delta, a novel cadmium-responsive proto-oncogene.
Authors Joseph Pius; Lei Yi-Xiong; Whong Wen-Zong; Ong Tong-Man;
JournalJ Biol Chem
PubMed ID11711542
'The molecular mechanisms potentially responsible for cadmium-induced cell transformation and tumorigenesis were investigated using Balb/c-3T3 cells transformed with cadmium chloride. Differential display analysis of gene expression revealed consistent and reproducible overexpression of a transcript in the transformed cells compared with the nontransformed cells. The full-length cDNA corresponding to the differentially ... More
Identification of the novel K15 gene at the rightmost end of the Kaposi's sarcoma-associated herpesvirus genome.
AuthorsChoi JK, Lee BS, Shim SN, Li M, Jung JU.
JournalJ Virol
PubMed ID10590133
'Kaposi''s sarcoma-associated herpesvirus (KSHV) encodes a distinct open reading frame called K15 at a position equivalent to the gene encoding LMP2A of Epstein-Barr virus (EBV). K15 isolates from body cavity-based lymphoma (BCBL) cells exhibited a dramatic sequence variation and a complex splicing pattern. However, all K15 alleles are organized similarly ... More
Cloning and characterization of a family of proteins associated with Mpl.
'Thrombopoietin (TPO) controls the formation of megakaryocytes and platelets from hematopoietic stem cells via activation of the c-Mpl receptor and multiple downstream signal transduction pathways. We used two-hybrid screening to identify new proteins that interacted with the cytoplasmic domain of Mpl, and we found a new family of proteins designated ... More
Insulin Activates CCAAT/Enhancer Binding Proteins and Proinflammatory Gene Expression through the Phosphatidylinositol 3-Kinase Pathway in Vascular Smooth Muscle Cells.
'Phosphatidylinositol 3-kinase (PI3K) is a key molecule mediating signals of insulin in vascular smooth muscle cells (VSMCs). To examine the effect of chronic activation of PI3K on the gene expression of VSMCs, membrane-targeted p110CAAX, a catalytic subunit of PI3K, was overexpressed in rat VSMCs by adenovirus-mediated gene transfer. Similar to ... More
The Tight Junction-specific Protein Occludin Is a Functional Target of the E3 Ubiquitin-protein Ligase Itch.
'Tight junctions create a highly selective diffusion barrier between epithelial and endothelial cells by preventing the free passage of molecules and ions across the paracellular pathway. Although the regulation of this barrier is still enigmatic, there is evidence that junctional transmembrane proteins are critically involved. Recent evidence confirms the notion ... More
Evolution of moth sex pheromones via ancestral genes.
Authors Roelofs Wendell L; Liu Weitian; Hao Guixia; Jiao Hongmei; Rooney Alejandro P; Linn Charles E Jr;
JournalProc Natl Acad Sci U S A
PubMed ID12374851
'Mate finding in most moth species involves long-distance signaling via female-emitted sex pheromones. There is a great diversity of pheromone structures used throughout the Lepidoptera, even among closely related species. The conundrum is how signal divergence has occurred. With strong normalizing selection pressure on blend composition and response preferences, it ... More
Ras Signaling Is Required for Serum-Induced HyphalDifferentiation in Candida albicans.
AuthorsFeng Q, Summers E, Guo B, Fink G
JournalJ Bacteriol
PubMed ID10515923
'Serum induces Candida albicans to make a rapid morphological change from the yeast cell form tohyphae. Contrary to the previous reports, we found that serum albumin does not play a critical role in thismorphological change. Instead, a filtrate (molecular mass, <1 kDa) devoid of serum albumin induceshyphae. To study genes ... More
Reduced adaptation of a non-recombining neo-Y chromosome.
Authors Bachtrog Doris; Charlesworth Brian;
JournalNature
PubMed ID11907578
'Sex chromosomes are generally believed to have descended from a pair of homologous autosomes. Suppression of recombination between the ancestral sex chromosomes led to the genetic degeneration of the Y chromosome. In response, the X chromosome may become dosage-compensated. Most proposed mechanisms for the degeneration of Y chromosomes involve the ... More
Role of SCF ubiquitin-ligase and the COP9 signalosome in the N gene-mediated resistance response to Tobacco mosaic virus.
AuthorsLiu Y, Schiff M, Serino G, Deng XW, Dinesh-Kumar SP,
JournalPlant Cell
PubMed ID12119369
'The tobacco N gene confers resistance to Tobacco mosaic virus (TMV) and encodes a toll-interleukin-1 receptor/nucleotide binding/Leu-rich repeat class protein. Recent evidence indicates that the Nicotiana benthamiana Rar1 gene (NbRar1), which encodes a protein with a zinc finger motif called CHORD (Cys- and His-rich domain), is required for the function ... More
Interaction of SAP97 with minus-end-directed actin motor myosin VI. Implications for AMPA receptor trafficking.
'SAP97 is a modular protein composed of three PDZ domains, an SH3 domain, and a guanylate kinase-like domain. It has been implicated functionally in the assembly and structural stability of synaptic junctions as well as in the trafficking, recruitment, and localization of specific ion channels and neurotransmitter receptors. The N ... More
Cloning of a novel phosphatidylinositol kinase-related kinase: characterization of the human SMG-1 RNA surveillance protein.
Authors Denning G; Jamieson L; Maquat L E; Thompson E A; Fields A P;
JournalJ Biol Chem
PubMed ID11331269
'We have cloned and characterized a new member of the phosphatidylinositol kinase (PIK)-related kinase family. This gene, which we term human SMG-1 (hSMG-1), is orthologous to Caenorhabditis elegans SMG-1, a protein that functions in nonsense-mediated mRNA decay (NMD). cDNA sequencing revealed that hSMG-1 encodes a protein of 3031 amino acids ... More
Calcineurin-dependent regulation of Crz1p nuclear export requires Msn5p and a conserved calcineurin docking site.
Authors Boustany Leila M; Cyert Martha S;
JournalGenes Dev
PubMed ID11877380
'Calcineurin, a conserved Ca(2+)/calmodulin-regulated protein phosphatase, plays a crucial role in Ca(2+) signaling in a wide variety of cell types. In Saccharomyces cerevisiae, calcineurin positively regulates transcription in response to stress by dephosphorylating the transcription factor Crz1p/Tcn1p. Dephosphorylation promotes Crz1p nuclear localization in part by increasing the efficiency of its ... More
Efficacy, safety, and pharmacokinetics of imatinib dose escalation to 800 mg/day in patients with advanced gastrointestinal stromal tumors.
AuthorsYoo C, Ryu MH, Ryoo BY, Beck MY, Kang YK
JournalInvest New Drugs
PubMed ID23591629
'Imatinib dose escalation has been suggested as an effective therapy for advanced gastrointestinal stromal tumors (GIST) after progression on the standard dose. We evaluated the efficacy, tolerability, and pharmacokinetics of imatinib dose escalation. Eighty-four patients with GIST who received imatinib 800 mg/day as second-line therapy were reviewed. In 66 patients, imatinib ... More
FIV infection of IL-2 -dependant and -independent feline lymphocyte lines: Host cells range distinctions and specific cytokine upregulation.
AuthorsLerner, D.L., Grant, C.K., deParseval, A., and Elder, J.H.
JournalVeterinary Immunology and Immunopathology
PubMed ID9839880
'We have analyzed the ability of three molecular clones of feline immunodeficiency virus (FIV) and an ex vivo variant to infect nine distinct specific-pathogen-free feline cell lines in tissue culture. The purpose of these studies was to elucidate mechanisms by which host cells regulate the level of virus infection and ... More
Use of cell-SELEX to generate DNA aptamers as molecular probes of HPV-associated cervical cancer cells.
AuthorsGraham JC, Zarbl H
JournalPLoS One
PubMed ID22536456
'Disease-specific biomarkers are an important tool for the timely and effective management of pathological conditions, including determination of susceptibility, diagnosis, and monitoring efficacy of preventive or therapeutic strategies. Aptamers, comprising single-stranded or double-stranded DNA or RNA, can serve as biomarkers of disease or biological states. Aptamers can bind to specific ... More
A genome-wide functional assay of signal transduction in living mammalian cells.
AuthorsWhitney M, Rockenstein E, Cantin G, Knapp T, Zlokarnik G, Sanders P, Durick K, Craig FF, Negulescu PA
JournalNat Biotechnol
PubMed ID9853613
'We describe a genome-wide functional assay for rapid isolation of cell clones and genetic elements responsive to specific stimuli. A promoterless beta-lactamase reporter gene was transfected into a human T-cell line to generate a living library of reporter-tagged clones. When loaded with a cell-permeable fluorogenic substrate, the cell library simultaneously ... More
Autochthonous eukaryotic diversity in hydrothermal sediment and experimental microcolonizers at the Mid-Atlantic Ridge.
AuthorsLópez-GarcÃa P, Philippe H, Gail F, Moreira D,
JournalProc Natl Acad Sci U S A
PubMed ID12522264
'The diversity and mode of life of microbial eukaryotes in hydrothermal systems is very poorly known. We carried out a molecular survey based on 18S ribosomal RNA genes of eukaryotes present in different hydrothermal niches at the Mid-Atlantic Ridge. These included metal-rich and rare-earth-element-rich hydrothermal sediments of the Rainbow site, ... More
3-Hydroxykynurenine Transaminase Identity with Alanine Glyoxylate Transaminase. A PROBABLE DETOXIFICATION PROTEIN IN AEDES AEGYPTI.
Authors Han Qian; Fang Jianmin; Li Jianyong;
JournalJ Biol Chem
PubMed ID11880382
'This study describes the functional characterization of a specific mosquito transaminase responsible for catalyzing the transamination of 3-hydroxykynurenine (3-HK) to xanthurenic acid (XA). The enzyme was purified from Aedes aegypti larvae by ammonium sulfate fractionation, heat treatment, and various chromatographic techniques, plus non-denaturing electrophoresis. The purified transaminase has a relative ... More
An mRNA surveillance mechanism that eliminates transcripts lacking termination codons.
Authors Frischmeyer Pamela A; van Hoof Ambro; O'Donnell Kathryn; Guerrerio Anthony L; Parker Roy; Dietz Harry C;
JournalScience
PubMed ID11910109
Translation is an important mechanism to monitor the quality of messenger RNAs (mRNAs), as exemplified by the translation-dependent recognition and degradation of transcripts harboring premature termination codons (PTCs) by the nonsense-mediated mRNA decay (NMD) pathway. We demonstrate in yeast that mRNAs lacking all termination codons are as labile as nonsense ... More
A molecular map of T cell development.
AuthorsSant'Angelo DB, Lucas B, Waterbury PG, Cohen B, Brabb T, Goverman J, Germain RN, Janeway CA Jr
JournalImmunity
PubMed ID9729038
Using a sensitive molecular marker for positive selection, the appearance of a particular functional TCR alpha chain sequence in cells from mice bearing a transgenic beta chain, we address several aspects of intrathymic T cell development. First, by examining specific TCR prior to and after maturation, we demonstrate how a ... More
The Solution Structure of Acyl Carrier Protein from Mycobacterium tuberculosis.
Authors Wong Hing C; Liu Gaohua; Zhang Yong-Mei; Rock Charles O; Zheng Jie;
JournalJ Biol Chem
PubMed ID11825906
Acyl carrier protein (ACP) performs the essential function of shuttling the intermediates between the enzymes that constitute the type II fatty acid synthase system. Mycobacterium tuberculosis is unique in producing extremely long mycolic acids, and tubercular ACP, AcpM, is also unique in possessing a longer carboxyl terminus than other ACPs. ... More
Origin of mitochondria in relation to evolutionary history of eukaryotic alanyl-tRNA synthetase.
AuthorsChihade JW, Brown JR, Schimmel PR, Ribas De Pouplana L
JournalProc Natl Acad Sci U S A
PubMed ID11035802
The origin of the eukaryotic cell remains an unsolved question. Numerous experimental and phylogenetic observations support the symbiotic origin of the modern eukaryotic cell, with its nucleus and (typically) mitochondria. Incorporation of mitochondria has been proposed to precede development of the nucleus, but it is still unclear whether mitochondria ... More
Mariner-like transposases are widespread and diverse in flowering plants.
Complete and partial sequences of mariner-like elements (MLEs) have been reported for hundreds of species of animals, but only two have been identified in plants. On the basis of these two plant MLEs and several related sequences identified by database searches, plant-specific degenerate primers were derived and used to amplify ... More
Genetic engineering of phytochrome biosynthesis in bacteria.
Authors Gambetta G A; Lagarias J C;
JournalProc Natl Acad Sci U S A
PubMed ID11553807
The bilin prosthetic groups of the phytochrome photoreceptors and the light-harvesting phycobiliprotein antennae arise from the oxygen-dependent ring opening of heme. Two ferredoxin-dependent enzymes contribute to this conversion: a heme oxygenase and a bilin reductase with discrete double-bond specificity. Using a dual plasmid system, one expressing a truncated cyanobacterial apophytochrome ... More
Two distinct pseudomonas effector proteins interact with the pto kinase and activate plant immunity.
Authors Kim Young Jin; Lin Nai Chun; Martin Gregory B;
JournalCell
PubMed ID12062102
The Pto serine/threonine kinase of tomato confers resistance to speck disease by recognizing strains of Pseudomonas syringae that express the protein AvrPto. Pto and AvrPto physically interact, and this interaction is required for activation of host resistance. We identified a second Pseudomonas protein, AvrPtoB, that interacts specifically with Pto and ... More
Shielding of the A1 Domain by the D'D3 Domains of von Willebrand Factor Modulates Its Interaction with Platelet Glycoprotein Ib-IX-V.
AuthorsUlrichts H, Udvardy M, Lenting PJ, Pareyn I, Vandeputte N, Vanhoorelbeke K, Deckmyn H,
JournalJ Biol Chem
PubMed ID16373331
Soluble von Willebrand factor (VWF) has a low affinity for platelet glycoprotein (GP) Ibalpha and needs immobilization and/or high shear stress to enable binding of its A1 domain to the receptor. The previously described anti-VWF monoclonal antibody 1C1E7 enhances VWF/GPIbalpha binding and recognizes an epitope in the amino acids 764-1035 ... More
The Salmonella enterica serovar typhimurium-encoded type III secretion systems can translocate Chlamydia trachomatis proteins into the cytosol of host cells.
AuthorsHo TD, Starnbach MN,
JournalInfect Immun
PubMed ID15664932
Chlamydia trachomatis is an obligate, intracellular pathogen that is a major cause of preventable blindness and infertility worldwide. Although the published genome sequence suggests that C. trachomatis encodes a type III secretion system, the lack of genetic tools for studying Chlamydia has hindered the examination of this potentially important class ... More
Endothelial induction of fgl2 contributes to thrombosis during acute vascular xenograft rejection.
AuthorsGhanekar A, Mendicino M, Liu H, He W, Liu M, Zhong R, Phillips MJ, Levy GA, Grant DR,
JournalJ Immunol
PubMed ID15100314
Thrombosis is a prominent feature of acute vascular rejection (AVR), the current barrier to survival of pig-to-primate xenografts. Fibrinogen-like protein 2 (fgl2/fibroleukin) is an inducible prothrombinase that plays an important role in the pathogenesis of fibrin deposition during viral hepatitis and cytokine-induced fetal loss. We hypothesized that induction of fgl2 ... More
Evolutionary divergence of the archaeal aspartyl-tRNA synthetases into discriminating and nondiscriminating forms.
Asparaginyl-tRNA (Asn-tRNA) is generated in nature via two alternate routes, either direct acylation of tRNA with asparagine by asparaginyl-tRNA synthetase (AsnRS) or in a two-step pathway that requires misacylated Asp-tRNA(Asn) as an intermediate. This misacylated aminoacyl-tRNA is formed by a nondiscriminating aspartyl-tRNA synthetase (AspRS), an enzyme that in addition to ... More
A normal beta-globin allele as a modifier gene ameliorating the severity of alpha-thalassemia in mice.
AuthorsLeder A, Wiener E, Lee MJ, Wickramasinghe SN, Leder P
JournalProc Natl Acad Sci U S A
PubMed ID10339580
Thalassemia is a heritable human anemia caused by a variety of mutations that affect expression of the alpha- or the beta-chain of hemoglobin. The expressivity of the phenotype is likely to be influenced by unlinked modifying genes. Indeed, by using a mouse model of alpha-thalassemia, we find that its phenotype ... More
Genealogy of the CCR5 locus and chemokine system gene variants associated with altered rates of HIV-1 disease progression.
AuthorsMummidi S, Ahuja SS, Gonzalez E, Anderson SA, Santiago EN, Stephan KT, Craig FE, O'Connell P, Tryon V, Clark RA, Dolan MJ, Ahuja SK
JournalNat Med
PubMed ID9662369
Allelic variants for the HIV-1 co-receptors chemokine receptor 5 (CCR5) and CCR2, as well as the ligand for the co-receptor CXCR4, stromal- derived factor (SDF-1), have been associated with a delay in disease progression. We began this study to test whether polymorphisms in the CCR5 regulatory regions influence the course ... More
A new member of the mouse prolactin (PRL)-like protein-C subfamily, PRL- like protein-C alpha: structure and expression.
AuthorsDai G, Chapman BM, Liu B, Orwig KE, Wang D, White RA, Preuett B, Soares MJ
JournalEndocrinology
PubMed ID9832456
In this study, we establish the presence of a unique member of the PRL- like protein-C (PLP-C) subfamily in the mouse, PLP-C alpha, characterize its complementary DNA and gene, and map its chromosomal location and pattern of expression during pregnancy. Mouse PLP-C alpha encodes for a 239 amino acid protein ... More
Targeting Alzheimer's disease genes with RNA interference: an efficient strategy for silencing mutant alleles.
AuthorsMiller VM, Gouvion CM, Davidson BL, Paulson HL,
JournalNucleic Acids Res
PubMed ID14754988
Tau and amyloid precursor protein (APP) are key proteins in the pathogenesis of sporadic and inherited Alzheimer's disease. Thus, developing ways to inhibit production of these proteins is of great research and therapeutic interest. The selective silencing of mutant alleles, moreover, represents an attractive strategy for treating inherited dementias and ... More
The imprint of intrathymic self-peptides on the mature T cell receptor repertoire.
AuthorsSant'Angelo DB, Waterbury PG, Cohen BE, Martin WD, Van Kaer L, Hayday AC, Janeway CA Jr
JournalImmunity
PubMed ID9354472
The analysis of T cell receptor alpha (TCR alpha) chains in mice transgenic for a TCR beta chain has allowed us to demonstrate a central role for self-peptides in the positive intrathymic selection of major histocompatibility complex (MHC) class II-restricted T cells. Analysis of specific V alpha-J alpha joins in ... More
Missense mutations in desmin associated with familial cardiac and skeletal myopathy.
AuthorsGoldfarb LG, Park KY, Cervenakova L, Gorokhova S, Lee HS, Vasconcelos O, Nagle JW, Semino-Mora C, Sivakumar K, Dalakas MC
JournalNature Genetics
PubMed ID9697706
Desmin-related myopathy (OMIM 601419) is a familial disorder characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias and restrictive heart failure, and by intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells. The underlying molecular mechanisms are unknown. Involvement of the desmin gene (DES) has been ... More
Two widely spaced initiator binding sites create an HMG1-dependent parvovirus rolling-hairpinreplication origin.
AuthorsCotmore SF, Christensen J, Tattersall P
JournalJ Virol
PubMed ID10627544
Minute virus of mice (MVM) replicates via a linearized form of rolling-circle replication in which the viral nickase, NS1, initiates DNA synthesis by introducing a site-specific nick into either of two distinct origin sequences. In vitro nicking and replication assays with substrates that had deletions or mutations were used to ... More
Targeted manipulation of maize genes in vivo using chimeric RNA/DNA oligonucleotides
AuthorsZhu T, Peterson DJ, Tagliani L, St Clair G, Baszczynski CL, Bowen B
JournalProc Natl Acad Sci U S A
PubMed ID10411950
Site-specific heritable mutations in maize genes were engineered by introducing chimeric RNA/DNA oligonucleotides. Two independent targets within the endogenous maize acetohydroxyacid synthase gene sequence were modified in a site-specific fashion, thereby conferring resistance to either imidazolinone or sulfonylurea herbicides. Similarly, an engineered green fluorescence protein transgene was site-specifically modified in ... More
Elevated retinoic acid receptor beta(4) protein in human breast tumor cells with nuclear and cytoplasmic localization.
AuthorsSommer KM, Chen LI, Treuting PM, Smith LT, Swisshelm K
JournalProc Natl Acad Sci U S A
PubMed ID10411930
The transcription factor retinoic acid receptor beta(2) (RARbeta(2)) is a potent inhibitor of breast cancer cells in vitro, and studies suggest that RARbeta expression is lost in primary breast cancer. Although RARbeta(2) is selectively down-regulated at the mRNA level in breast tumor cells, we show that expression of an RARbeta ... More
Mechanisms of dCMP transferase reactions catalyzed by mouse Rev1 protein.
The Rev1 protein, a member of a large family of translesion DNA polymerases, catalyzes a dCMP transfer reaction. Recombinant mouse Rev1 protein was found to insert a dCMP residue opposite guanine, adenine, thymine, cytosine, uracil, and an apurinic/apyrimidinic site and to have weak ability for transfer to a mismatched terminus. ... More
Pathfinding and error correction by retinal axons: the role of astray/robo2.
Authors Hutson Lara D; Chien Chi Bin;
JournalNeuron
PubMed ID11804561
To address how the highly stereotyped retinotectal pathway develops in zebrafish, we used fixed-tissue and time-lapse imaging to analyze morphology and behavior of wild-type and mutant retinal growth cones. Wild-type growth cones increase in complexity and pause at the midline. Intriguingly, they make occasional ipsilateral projections and other pathfinding errors, ... More
The novel human DNA helicase hFBH1 is an F-box protein.
Authors Kim Jaehoon; Kim Jeong-Hoon; Lee Sung-Hak; Kim Do-Hyung; Kang Ho-Young; Bae Sung-Ho; Pan Zhen-Qiang; Seo Yeon-Soo;
JournalJ Biol Chem
PubMed ID11956208
We have identified a novel DNA helicase in humans that belongs to members of the superfamily I helicase and found that it contains a well conserved F-box motif at its N terminus. We have named the enzyme hFBH1 (human F-box DNA helicase 1). Recombinant hFBH1, containing glutathione S-transferase at the ... More
Cloning and functional characterization of a novel aquaporin from Xenopus laevis oocytes.
We have cloned a novel aquaporin (AQP) from Xenopus laevis oocytes, which we have provisionally named AQPxlo. The predicted protein showed highest homology (39-50%) to aquaglyceroporins. Northern blot analysis showed strong hybridization to an approximately 1.4-kb transcript in X. laevis fat body and oocytes, whereas a weaker signal was obtained ... More
A streamlined method for detecting structural variants in cancer genomes by short read paired-end sequencing.
AuthorsMijuškovic M, Brown SM, Tang Z, Lindsay CR, Efstathiadis E, Deriano L, Roth DB
JournalPLoS One
PubMed ID23144753
Defining the architecture of a specific cancer genome, including its structural variants, is essential for understanding tumor biology, mechanisms of oncogenesis, and for designing effective personalized therapies. Short read paired-end sequencing is currently the most sensitive method for detecting somatic mutations that arise during tumor development. However, mapping structural variants ... More
Infectivity-associated changes in the transcriptional repertoire of the malaria parasite sporozoite stage.
Authors Matuschewski Kai; Ross Jessica; Brown Stuart M; Kaiser Karine; Nussenzweig Victor; Kappe Stefan H I;
JournalJ Biol Chem
PubMed ID12177071
Injection of Plasmodium salivary gland sporozoites into the vertebrate host by Anopheles mosquitoes initiates malaria infection. Sporozoites develop within oocysts in the mosquito midgut and then enter and mature in the salivary glands. Although morphologically similar, oocyst sporozoites and salivary gland sporozoites differ strikingly in their infectivity to the mammalian ... More
Estradiol-induced nongenomic calcium signaling regulates genotropic signaling in macrophages.
Authors Guo Zhiyong; Krucken Jurgen; Benten W Peter M; Wunderlich Frank;
JournalJ Biol Chem
PubMed ID11751857
Estradiol (E(2)) exerts not only genotropic but also nongenomic actions through nuclear estrogen receptors (ER). Here, we provide a novel paradigm for nongenomic E(2) signaling independent of nuclear ER. E(2) induces a rapid rise in the intracellular free Ca(2+) concentration ([Ca(2+)](i)) through membrane estrogen receptors in murine RAW 264.7 macrophages. ... More
Nuclear translocation of insulin receptor substrate-1 by the simian virus 40 T antigen and the activated type 1 insulin-like growth factor receptor.
32D cells are a murine hemopoietic cell line that undergoes apoptosis upon withdrawal of interleukin-3 (IL-3) from the medium. 32D cells have low levels of the type 1 insulin-like growth factor (IGF-I) receptor and do not express insulin receptor substrate-1 (IRS-1) or IRS-2. Ectopic expression of IRS-1 delays apoptosis but ... More
Monoclonal mice generated by nuclear transfer from mature B and T donor cells.
Authors Hochedlinger Konrad; Jaenisch Rudolf;
JournalNature
PubMed ID11875572
Cloning from somatic cells is inefficient, with most clones dying during gestation. Cloning from embryonic stem (ES) cells is much more effective, suggesting that the nucleus of an embryonic cell is easier to reprogram. It is thus possible that most surviving clones are, in fact, derived from the nuclei of ... More
Cysteinyl-tRNA synthetase is not essential for viability of the archaeon Methanococcus maripaludis.
Authors Stathopoulos C; Kim W; Li T; Anderson I; Deutsch B; Palioura S; Whitman W; Söll D;
JournalProc Natl Acad Sci U S A
PubMed ID11717392
The methanogenic archaea Methanocaldococcus jannaschii and Methanothermobacter thermautotrophicus contain a dual-specificity prolyl-tRNA synthetase (ProCysRS) that accurately forms both prolyl-tRNA (Pro-tRNA) and cysteinyl-tRNA (Cys-tRNA) suitable for in vivo translation. This intriguing enzyme may even perform its dual role in organisms that possess a canonical single-specificity cysteinyl-tRNA synthetase (CysRS), raising the question ... More
Antidiabetic Effect of a Prodrug of Cysteine, L-2-Oxothiazolidine-4-carboxylic Acid, through CD38 Dimerization and Internalization.
Authors Han Myung-Kwan; Kim Se-Jin; Park Young-Ran; Shin Young-Mi; Park Hyun-Jung; Park Kum-Jae; Park Kwang-Hyun; Kim Hyun-Kag; Jang Seon-Il; An Nyeon-Hyoung; Kim Uh-Hyun;
JournalJ Biol Chem
PubMed ID11679582
CD38 is a bifunctional enzyme synthesizing (ADP-ribosyl cyclase) and degrading (cyclic ADP-ribose (cADPR) hydrolase) cADPR, a potent Ca(2+) mobilizer from intracellular pools. CD38 internalization has been proposed as a mechanism by which the ectoenzyme produced intracellular cADPR, and thiol compounds have been shown to induce the internalization of CD38. Here, ... More
Mutations of CD40 gene cause an autosomal recessive form of immunodeficiency with hyper IgM.
Authors Ferrari S; Giliani S; Insalaco A; Al-Ghonaium A; Soresina A R; Loubser M; Avanzini M A; Marconi M; Badolato R; Ugazio A G; Levy Y; Catalan N; Durandy A; Tbakhi A; Notarangelo L D; Plebani A;
JournalProc Natl Acad Sci U S A
PubMed ID11675497
CD40 is a member of the tumor necrosis factor receptor superfamily, expressed on a wide range of cell types including B cells, macrophages, and dendritic cells. CD40 is the receptor for CD40 ligand (CD40L), a molecule predominantly expressed by activated CD4(+) T cells. CD40/CD40L interaction induces the formation of memory ... More
Gene characterized for membrane desaturase that produces (E)-11 isomers of mono- and diunsaturated fatty acids.
Moth species have evolved integral membrane desaturases that exhibit a wide diversity in substrate specificity, as well as in regiospecificity and stereospecificity of the unsaturated products. We report here the cloning and expression of a single desaturase from the sex pheromone gland of the light brown apple moth, Epiphyas postvittana, ... More
AKAP350 at the Golgi apparatus. I. Identification of a distinct Golgi apparatus targeting motif in AKAP350.
Authors Shanks Ryan A; Steadman Brent T; Schmidt P Henry; Goldenring James R;
JournalJ Biol Chem
PubMed ID12163481
The protein kinase A-anchoring proteins (AKAPs) are defined by their ability to scaffold protein kinase A to specific subcellular compartments. Each of the AKAP family members utilizes unique targeting domains specific for a particular subcellular compartment. AKAP350 is a multiply spliced AKAP family member localized to the centrosome and the ... More
The p66Shc longevity gene is silenced through epigenetic modifications of an alternative promoter.
The mammal Shc locus encodes three overlapping isoforms (46, 52, and 66 kDa) that differ in the length of their N-terminal regions. p46/p52Shc and p66Shc have been implicated, respectively, in the cytoplasmic propagation of growth and apoptogenic signals. Levels of p66Shc expression correlate with life span duration in mice. p46Shc ... More
Allurin, a 21-kDa sperm chemoattractant from Xenopus egg jelly, is related to mammalian sperm-binding proteins.
Authors Olson J H; Xiang X; Ziegert T; Kittelson A; Rawls A; Bieber A L; Chandler D E;
JournalProc Natl Acad Sci U S A
PubMed ID11562501
Previously, we demonstrated that a protein from Xenopus egg jelly exhibits sperm chemoattractant activity when assayed by either video microscopy or by sperm passage across a porous filter. Here we describe the isolation and purification of allurin, the protein responsible for this activity. Freshly oviposited jellied eggs were soaked in ... More
A. thaliana TRANSPARENT TESTA 1 is involved in seed coat development and defines the WIP subfamily of plant zinc finger proteins.
Authors Sagasser Martin; Lu Gui-Hua; Hahlbrock Klaus; Weisshaar Bernd;
JournalGenes Dev
PubMed ID11782451
Seeds of the Arabidopsis thaliana transparent testa 1 mutant (tt1) appear yellow, due to the lack of condensed tannin pigments in the seed coat. The TT1 gene was isolated by reverse genetics using an En-1 transposon mutagenized A. thaliana population. TT1 gene expression was detected in developing ovules and young ... More
Validated zinc finger protein designs for all 16 GNN DNA triplet targets.
Authors Liu Qiang; Xia ZhenQin; Case Casey C;
JournalJ Biol Chem
PubMed ID11726671
The Cys(2)-His(2)-type zinc finger DNA-binding proteins can be engineered to bind specifically to many different DNA sequences. A single zinc finger typically binds to a 3-4-base pair DNA subsite. One strategy for design is to identify highly specific fingers that recognize each of the 64 possible DNA triplets. We started ... More
Direct interaction between uracil-DNA glycosylase and a proliferating cell nuclear antigen homolog in the crenarchaeon Pyrobaculum aerophilum.
Authors Yang Hanjing; Chiang Ju-Huei; Fitz-Gibbon Sorel; Lebel Michel; Sartori Alessandro A; Jiricny Joseph; Slupska Malgorzata M; Miller Jeffrey H;
JournalJ Biol Chem
PubMed ID11927597
Proliferating cell nuclear antigen (PCNA) acts as a sliding clamp on duplex DNA. Its homologs, present in Eukarya and Archaea, are part of protein complexes that are indispensable for DNA replication and DNA repair. In Eukarya, PCNA is known to interact with more than a dozen different proteins, including a ... More
A novel zinc-regulated human zinc transporter, hZTL1, is localized to the enterocyte apical membrane.
Zinc is essential to a wide range of cellular processes; therefore, it is important to elucidate the molecular mechanisms of zinc homeostasis. To date, no zinc transporters expressed at the enterocyte apical membrane, and so essential to mammalian zinc homeostasis, have been discovered. We identified hZTL1 as a human expressed ... More
Functional characterization of three novel tissue-specific anion exchangers SLC26A7, -A8, and -A9.
A second distinct family of anion exchangers, SLC26, in addition to the classical SLC4 (or anion exchanger) family, has recently been delineated. Particular interest in this gene family is stimulated by the fact that the SLC26A2, SLC26A3, and SLC26A4 genes have been recognized as the disease genes mutated in diastrophic ... More
Mutations in the yeast mitochondrial RNA polymerase specificity factor, mtf1, verify an essential role in promoter utilization.
Authors Karlok Mark A; Jang Sei-Heon; Jaehning Judith A;
JournalJ Biol Chem
PubMed ID12021282
The yeast mitochondrial RNA polymerase (RNAP) is a two-subunit enzyme composed of a catalytic core (Rpo41) and a specificity factor (Mtf1) encoded by nuclear genes. Neither subunit on its own interacts with promoter DNA, but the combined holo-RNAP recognizes and selectively initiates from promoters related to the consensus sequence ATATAAGTA. ... More
A Novel Zinc Finger Protein That Inhibits Osteoclastogenesis and the Function of Tumor Necrosis Factor Receptor-associated Factor 6.
Authors Shin Jin Na; Kim Injune; Lee Jung Sup; Koh Gou Young; Lee Zang Hee; Kim Hong-Hee;
JournalJ Biol Chem
PubMed ID11751921
A variety of surface receptors eliciting diverse cellular responses have been shown to recruit tumor necrosis factor receptor-associated factor (TRAF) adaptor molecules. However, a few TRAF-interacting intracellular proteins that serve as downstream targets or regulators of TRAF function have been identified. In search of new intracellular molecules that bind TRAF6, ... More
Up-regulation of Prostaglandin E2 Synthesis by Interleukin-1beta in Human Orbital Fibroblasts Involves Coordinate Induction of Prostaglandin-Endoperoxide H Synthase-2 and Glutathione-dependent Prostaglandin E2 Synthase Expression.
Authors Han Rui; Tsui Shanli; Smith Terry J;
JournalJ Biol Chem
PubMed ID11847219
Prostaglandin E(2) (PGE(2)) production involves the activity of a multistep biosynthetic pathway. The terminal components of this cascade, two PGE(2) synthases (PGES), have very recently been identified as glutathione-dependent proteins. cPGES is cytoplasmic, apparently identical to the hsp90 chaperone, p23, and associates functionally with prostaglandin-endoperoxide H synthase-1 (PGHS-1), the constitutive ... More
BAG4/SODD protein contains a short BAG domain.
AuthorsBriknarova K, Takayama S, Homma S, Baker K, Cabezas E, Hoyt DW, Li Z, Satterthwait AC, Ely KR.
JournalJ Biol Chem
PubMed ID12058034
BAG (Bcl-2-associated athanogene) proteins are molecular chaperone regulators that affect diverse cellular pathways. All members share a conserved motif, called the BAG domain (BD), which binds to Hsp70/Hsc70 family proteins and modulates their activity. We have determined the solution structure of BD from BAG4/SODD (silencer of death domains) by multidimensional ... More
A third bacterial system for the assembly of iron-sulfur clusters with homologs in archaea and plastids.
Authors Takahashi Yasuhiro; Tokumoto Umechiyo;
JournalJ Biol Chem
PubMed ID12089140
The assembly of iron-sulfur (Fe-S) clusters is mediated by complex machinery. In several proteobacteria, this process involves ISC (Fe-S cluster assembly) machinery composed of at least six components also conserved in mitochondria from lower to higher eukaryotes. In nitrogen-fixing bacteria, another system, termed NIF (nitrogen fixation), is required for the ... More
Nek11, a new member of the NIMA family of kinases, involved in DNA replication and genotoxic stress responses.
AuthorsNoguchi K, Fukazawa H, Murakami Y, Uehara Y
JournalJ Biol Chem
PubMed ID12154088
DNA replication and genotoxic stresses activate various checkpoint-associated protein kinases, and checkpoint dysfunction often leads to cell lethality. Here, we have identified new members of the mammalian NIMA family of kinases, termed Nek11L and Nek11S (NIMA-related kinase 11 Long and Short isoform) as novel DNA replication/damage stresses-responsive kinases. Molecular cloning ... More
Recombinant human laminin-10 (alpha5beta1gamma1). Production, purification, and migration-promoting activity on vascular endothelial cells.
The laminin (LN) family of large heterotrimeric extracellular matrix glycoproteins has multiple functions: LNs take part in the regulation of processes such as cell migration, differentiation, and proliferation, in addition to contributing to the structure of basement membranes. LN-10, composed of alpha5, beta1, and gamma1 chains, is widely distributed in ... More
Mutations of the gene encoding the protein kinase A type I-alpha regulatory subunit in patients with the Carney complex.
Authors Kirschner L S; Carney J A; Pack S D; Taymans S E; Giatzakis C; Cho Y S; Cho-Chung Y S; Stratakis C A;
JournalNat Genet
PubMed ID10973256
Carney complex (CNC) is a multiple neoplasia syndrome characterized by spotty skin pigmentation, cardiac and other myxomas, endocrine tumours and psammomatous melanotic schwannomas. CNC is inherited as an autosomal dominant trait and the genes responsible have been mapped to 2p16 and 17q22-24 (refs 6, 7). Because of its similarities to ... More
Isolation and Characterization of the Putative Nuclear Modifier Gene MTO1 Involved in the Pathogenesis of Deafness-associated Mitochondrial 12 S rRNA A1555G Mutation.
Authors Li Xiaoming; Li Ronghua; Lin Xinhua; Guan Min-Xin;
JournalJ Biol Chem
PubMed ID12011058
The human mitochondrial 12 S rRNA A1555G mutation has been found to be associated with aminoglycoside-induced and non-syndromic deafness. However, putative nuclear modifier gene(s) have been proposed to regulate the phenotypic expression of this mutation. In yeast, the mutant alleles of MTO1, encoding a mitochondrial protein, manifest respiratory-deficient phenotype only ... More
The synthesis of inositol hexakisphosphate. Characterization of human inositol 1,3,4,5,6-pentakisphosphate 2-kinase.
Authors Verbsky John W; Wilson Monita P; Kisseleva Marina V; Majerus Philip W; Wente Susan R;
JournalJ Biol Chem
PubMed ID12084730
The enzyme(s) responsible for the production of inositol hexakisphosphate (InsP(6)) in vertebrate cells are unknown. In fungal cells, a 2-kinase designated Ipk1 is responsible for synthesis of InsP(6) by phosphorylation of inositol 1,3,4,5,6-pentakisphosphate (InsP(5)). Based on limited conserved sequence motifs among five Ipk1 proteins from different fungal species, we have ... More
Altered levels of growth-related and novel gene transcripts in reproductive and other tissues of female mice overexpressing spermidine/spermine N1-acetyltransferase (SSAT).
Authors Min Seok Hong; Simmen Rosalia C M; Alhonen Leena; Halmekyto Maria; Porter Carl W; Janne Juhani; Simmen Frank A;
JournalJ Biol Chem
PubMed ID11709547
Overexpression of SSAT (polyamine catabolic enzyme) in female mice results in impaired ovarian folliculogenesis and uterine hypoplasia. To identify the molecular basis for this, the gene expression profiles in uterus and ovary and for comparison, liver and kidney, from non-transgenic (NT) and SSAT transgenic (ST) mice were compared. The mRNA ... More
The Clostridium ramosum IgA proteinase represents a novel type of metalloendopeptidase.
Clostridium ramosum is part of the normal flora in the human intestine. Some strains produce an IgA proteinase that specifically cleaves human IgA1 and the IgA2m(1) allotype. This prolylendopeptidase was purified from a broth culture supernatant, and N-terminal sequences of the native protein and tryptic fragments thereof were determined. A ... More
Cytoplasmic Prostaglandin E2 Synthase Is Dominantly Expressed in Cultured KAT-50 Thyrocytes, Cells That Express Constitutive Prostaglandin-endoperoxide H Synthase-2. BASIS FOR LOW PROSTAGLANDIN E2 PRODUCTION.
Authors Han Rui; Smith Terry J;
JournalJ Biol Chem
PubMed ID12145315
The recent identification and cloning of two glutathione-dependent prostaglandin E(2) synthase (PGES) genes has yielded important insights into the terminal step of PGE(2) synthesis. These enzymes form efficient functional pairs with specific members of the prostaglandin-endoperoxide H synthase (PGHS) family. Microsomal PGES (mPGES) is inducible and works more efficiently with ... More
Molecular cloning and characterization of the human diacylglycerol kinase beta (DGKbeta) gene: alternative splicing generates DGKbeta isotypes with different properties.
Authors Caricasole Andrea; Bettini Ezio; Sala Cinzia; Roncarati Renza; Kobayashi Naoki; Caldara Fabrizio; Goto Kaoru; Terstappen Georg C;
JournalJ Biol Chem
PubMed ID11719522
Diacylglycerol kinases are key modulators of levels of diacylglycerol, a second messenger involved in a variety of cellular responses to extracellular stimuli. A number of diacylglycerol kinases encoded by separate genes are present in mammalian genomes. We have cloned cDNAs encoding several isoforms of the human homologue of the rat ... More
Haemophilus influenzae type b strain A2 has multiple sialyltransferases involved in lipooligosaccharide sialylation.
Authors Jones Paul A; Samuels Nicole M; Phillips Nancy J; Munson Robert S Jr; Bozue Joel A; Arseneau Julie A; Nichols Wade A; Zaleski Anthony; Gibson Bradford W; Apicella Michael A;
JournalJ Biol Chem
PubMed ID11842084
The lipooligosaccharide (LOS) of Haemophilus influenzae contains sialylated glycoforms, and a sialyltransferase, Lic3A, has been previously identified. We report evidence for two additional sialyltransferases, SiaA, and LsgB, that affect N-acetyllactosamine containing glycoforms. Mutations in genes we have designated siaA and lsgB affected only the sialylated glycoforms containing N-acetylhexosamine. A mutation ... More
The WNT7b promoter is regulated by TTF-1, GATA6, and Foxa2 in lung epithelium.
Authors Weidenfeld Joel; Shu Weiguo; Zhang Lili; Millar Sarah E; Morrisey Edward E;
JournalJ Biol Chem
PubMed ID11914369
In this study, we find that WNT7b is the only member of the WNT family of autocrine/paracrine signaling molecules whose expression in the lung is restricted to the airway epithelium during embryonic development. To study the transcriptional mechanisms that underlie this restricted pattern of WNT7b expression, we isolated the proximal ... More
A transmembrane tight junction protein selectively expressed on endothelial cells and platelets.
Searching for cell surface proteins expressed at interendothelial cell contacts, we have raised monoclonal antibodies against intact mouse endothelial cells. We obtained two monoclonal antibodies, 1G8 and 4C10, that stain endothelial cell contacts and recognize a protein of 55 kDa. Purification and identification by mass spectrometry of this protein revealed ... More