ProBond™ Purification System - Citations

ProBond™ Purification System - Citations

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Citations & References
Abstract
Cellular uptake of saposin (SAP) precursor and lysosomal delivery by the low density lipoprotein receptor-related protein (LRP).
AuthorsHiesberger T, Huttler S, Rohlmann A, Schneider W, Sandhoff K, Herz J
JournalEMBO J
PubMed ID9707421
'Sphingolipid activator proteins SAP-A, -B, -C and -D (also called saposins) are generated by proteolytic processing from a 73 kDa precursor and function as obligatory activators of lysosomal enzymes involved in glycosphingolipid metabolism. Although the SAP precursor can be recognized by the mannose-6-phosphate (M-6-P) receptor and shuttled directly from the ... More
RasGRP4, a new mast cell-restricted Ras guanine nucleotide-releasing protein with calcium- and diacylglycerol-binding motifs. Identification of defective variants of this signaling protein in asthma, mastocytosis, and mast cell leukemia patients and demonstration of the importance of RasGRP4 in mast cell development and function.
Authors Yang Yi; Li Lixin; Wong Guang W; Krilis Steven A; Madhusudhan M S; Sali Andrej; Stevens Richard L;
JournalJ Biol Chem
PubMed ID11956218
'A cDNA was isolated from interleukin 3-developed, mouse bone marrow-derived mast cells (MCs) that contained an insert (designated mRasGRP4) that had not been identified in any species at the gene, mRNA, or protein level. By using a homology-based cloning approach, the approximately 2.6-kb hRasGRP4 transcript was also isolated from the ... More
Expression of a 28-kilodalton glutathione S-transferase antigen of Schistosoma mansoni on the surface of filamentous phages and evaluation of its vaccine potential.
AuthorsRao KV, He YX, Kalyanasundaram R,
JournalClin Diagn Lab Immunol
PubMed ID12853382
'A cloning and expression system that allows display of proteins on the surface of filamentous phages was exploited to display a 28-kDa glutathione S-transferase (Sm28GST) antigen of the human parasite Schistosoma mansoni. The phage-displayed Sm28GST (pdGST) was immunoreactive and was recognized by immune sera, suggesting that the Sm28GST protein displayed ... More
The cathepsin B of Toxoplasma gondii, toxopain-1, is critical for parasite invasion and rhoptry protein processing.
Authors Que Xuchu; Ngo Huân; Lawton Jeffrey; Gray Mary; Liu Qing; Engel Juan; Brinen Linda; Ghosh Partho; Joiner Keith A; Reed Sharon L;
JournalJ Biol Chem
PubMed ID12000756
'Cysteine proteinases play a major role in invasion and intracellular survival of a number of pathogenic parasites. We cloned a single copy gene, tgcp1, from Toxoplasma gondii and refolded recombinant enzyme to yield active proteinase. Substrate specificity of the enzyme and homology modeling identified the proteinase as a cathepsin B. ... More
Proapoptotic activity of Caenorhabditis elegans CED-4 protein in Drosophila: implicated mechanisms for caspase activation.
AuthorsKanuka H, Hisahara S, Sawamoto K, Shoji S, Okano H, Miura M
JournalProc Natl Acad Sci U S A
PubMed ID9874786
'CED-4 protein plays an important role in the induction of programmed cell death in Caenorhabditis elegans through the activation of caspases. However, the precise mechanisms by which it activates caspases remain unknown. To investigate the conservation of CED-4 function in evolution, transgenic Drosophila lines that express CED-4 in the compound ... More
Lymphocyte-specific protein 1 expression in eukaryotic cells reproduces the morphologic and motile abnormality of NAD 47/89 neutrophils.
AuthorsHoward TH, Hartwig J, Cunningham C
JournalBlood
PubMed ID9616178
'Despite its name, the actin-binding protein lymphocyte-specific protein1 (LSP1) is found in all hematopoetic cells, and yet its role in cell function remains unclear. Recently, LSP1 was identified as the 47- kD protein overexpressed in the polymorphonuclear neutrophils of patients with a rare neutrophil disorder, neutrophil actin dysfunction with abnormalities ... More
Induction of protective immunity against Schistosoma mansoni via DNA priming and boosting with the large subunit of calpain (Sm-p80): adjuvant effects of granulocyte-macrophage colony-stimulating factor and interleukin-4.
AuthorsSiddiqui AA, Phillips T, Charest H, Podesta RB, Quinlin ML, Pinkston JR, Lloyd JD, Paz M, Villalovos RM, Pompa J,
JournalInfect Immun
PubMed ID12819068
'Considerable morbidity and mortality result from schistosomiasis, an affliction affecting an estimated 200 million people. Although schistosomicidal drugs and other control measures (including public hygiene and snail control) exist, the advent of an efficacious vaccine remains the most potentially powerful means for controlling this disease. We have targeted a vaccine ... More
Metaxin is a component of a preprotein import complex in the outer membrane of the mammalian mitochondrion.
AuthorsArmstrong LC, Komiya T, Bergman BE, Mihara K, Bornstein P
JournalJ Biol Chem
PubMed ID9045676
'Metaxin, a novel gene located between the glucocerebrosidase and thrombospondin 3 genes in the mouse, is essential for survival of the postimplantation mouse embryo. In this study, the subcellular location, domain structure, and biochemical function of metaxin were investigated. Anti-recombinant metaxin antibodies recognized 35- and 70- kDa proteins in mitochondria ... More
Total synthesis of cyclic ADP-carbocyclic-ribose, a stable mimic of Ca2+-mobilizing second messenger cyclic ADP-ribose.
Authors Shuto S; Fukuoka M; Manikowsky A; Ueno Y; Nakano T; Kuroda R; Kuroda H; Matsuda A;
JournalJ Am Chem Soc
PubMed ID11535079
'The synthesis of cyclic ADP-carbocyclic-ribose (cADPcR, 4) designed as a stable mimic of cyclic ADP-ribose (cADPR, 1), a Ca2+-mobilizing second messenger, was achieved using as the key step a condensation reaction with the phenylthiophosphate-type substrate 14 to form an intramolecular pyrophosphate linkage. The N-1-carbocyclic-ribosyladenosine derivative 16 was prepared via the ... More
RSK2 Activity Is Regulated by Its Interaction with PEA-15.
AuthorsVaidyanathan H, Ramos JW,
JournalJ Biol Chem
PubMed ID12796492
'The ERK MAP (mitogen-activated protein) kinase cascade modulates many cellular processes including transcription, adhesion, growth, survival, and proliferation. One target substrate of ERK involved in regulating transcription is the p90 ribosomal S6 kinase (RSK) isozyme, RSK2. Here we demonstrate that a small death effector domain-containing protein called PEA-15 binds RSK2. ... More
Cloning of the human prolyl 4-hydroxylase alpha subunit isoform alpha(II) and characterization of the type II enzyme tetramer. The alpha(I) and alpha(II) subunits do not form a mixed alpha(I)alpha(II)beta2 tetramer.
AuthorsAnnunen P, Helaakoski T, Myllyharju J, Veijola J, Pihlajaniemi T, Kivirikko KI
JournalJ Biol Chem
PubMed ID9211872
'Prolyl 4-hydroxylase (proline hydroxylase, EC 1.14.11.2) catalyzes the formation of 4-hydroxyproline in collagens. The vertebrate enzyme is an alpha2beta2 tetramer, the beta subunit of which is identical to protein disulfide-isomerase (PDI, EC 5.3.4.1). We report here on cloning of the recently discovered alpha(II) subunit from human sources. The mRNA for ... More
The C-terminal region of Schizosaccaromyces pombe proliferating cell nuclear antigen is essential for DNA polymerase activity
AuthorsKelman Z, Zuo S, Arroyo MP, Wang TS, Hurwitz J
JournalNucleic Acids Res
PubMed ID10449724
'Proliferating cell nuclear antigen (PCNA), the processivity factor (sliding clamp) of DNA polymerases (Pols), plays essential roles in DNA metabolism. In this report, we examined the functional role of the C-terminal region of Schizosaccaromyces pombe PCNA both in vitro and in vivo. The deletion or Ala substitution of the last ... More
Mutational analysis of bovine viral diarrhea virus RNA-dependent RNA polymerase.
AuthorsLai VC, Kao CC, Ferrari E, Park J, Uss AS, Wright-Minogue J, Hong Z, Lau JY
JournalJ Virol
PubMed ID10559328
'Recombinant bovine viral diarrhea virus (BVDV) nonstructural protein 5B (NS5B) produced in insect cells has been shown to possess an RNA-dependent RNA polymerase (RdRp) activity. Our initial attempt to produce the full-length BVDV NS5B with a C-terminal hexahistidine tag in Escherichia coli failed due to the expression of insoluble products. ... More
Mutation at the catalytic site of topoisomerase I in CEM/C2, a human leukemia cell line resistant to camptothecin.
AuthorsFujimori A, Harker WG, Kohlhagen G, Hoki Y, Pommier Y
JournalCancer Res
PubMed ID7882333
'We developed previously a resistant cell line, CEM/C2, from the human leukemia cell line CCRF-CEM by stepwise selection in camptothecin. This cell line is 974-fold more resistant to camptothecin than parental cells. Resistance is only partially explained by 2-fold reductions in topoisomerase I protein and mRNA levels. We further investigated ... More
Comparison of kinetic properties between two mammalian ras p21 GDP/GTP exchange proteins, ras guanine nucleotide-releasing factor and smg GDP dissociation stimulation.
AuthorsOrita S, Kaibuchi K, Kuroda S, Shimizu K, Nakanishi H, Takai Y
JournalJ Biol Chem
PubMed ID8244990
'The mammalian counterpart of the yeast ras p21 GDP/GTP exchange protein CDC25, ras GRF, was expressed in Escherichia coli and purified, and its kinetic properties were compared with those of another mammalian ras p21 GDP/GTP exchange protein, smg GDS. ras GRF was active on Ki- and Ha- ras p21s but ... More
Neutrophil phospholipase D is activated by a membrane-associated Rho family small molecular weight GTP-binding protein.
AuthorsBowman EP, Uhlinger DJ, Lambeth JD
JournalJ Biol Chem
PubMed ID8408000
'Phospholipase D in human neutrophil lysates is activated by GTP gamma S (guanosine 5''-O-(3-thiotriphosphate)), implying the participation of a GTP-binding protein. Reconstitution of GTP gamma S-dependent activity requires protein factors in both the plasma membrane and the cytosol (Olson, S. C., Bowman, E. P., and Lambeth, J. D. (1991) J. ... More
Saccharomyces cerevisiae cytoplasmic tyrosyl-tRNA synthetase gene. Isolation by complementation of a mutant Escherichia coli suppressor tRNA defective in aminoacylation and sequence analysis.
AuthorsChow CM, RajBhandary UL
JournalJ Biol Chem
PubMed ID8509419
'Exploiting differences in tRNA recognition between prokaryotic and eukaryotic tyrosyl-tRNA synthetases (TyrRSs), we have isolated the gene for the cytoplasmic TyrRS of Saccharomyces cerevisiae by functional complementation in Escherichia coli of a mutant E. coli tRNA. The tRNA, derived from the E. coli initiator tRNA with changes to allow suppression ... More
Differential and Independent Roles of a 32 Homolog (RpoH) and an HrcA Repressor in the Heat Shock Response of Agrobacterium tumefaciens
AuthorsKenji Nakahigashi,1, Eliora Z. Ron,2 Hideki Yanagi,1 and Takashi Yura1,*
JournalJ Bacteriol
PubMed ID10601208
'The heat shock response in alpha proteobacteria is unique in that a combination of two regulators isinvolved: a positive regulator, RpoH (32 homolog), found in the alpha, beta, and gamma proteobacteria,and a negative regulator, HrcA, widely distributed in eubacteria but not in the gamma proteobacteria. Toassess the differential roles of ... More
Pseudomonas aeruginosa aspartate transcarbamoylase. Characterization of its catalytic and regulatory properties.
Authors Vickrey John F; Herve Guy; Evans David R;
JournalJ Biol Chem
PubMed ID11959858
'Aspartate transcarbamoylase from Pseudomonadaceae is a class A enzyme consisting of six copies of a 36-kDa catalytic chain and six copies of a 45-kDa polypeptide of unknown function. The 45-kDa polypeptide is homologous to dihydroorotase but lacks catalytic activity. Pseudomonas aeruginosa aspartate transcarbamoylase was overexpressed in Escherichia coli. The homogeneous ... More
Human T cell leukemia virus type 1 oncoprotein Tax targets the human mitotic checkpoint protein MAD1.
AuthorsJin DY, Spencer F, Jeang KT
JournalCell
PubMed ID9546394
'In searching for cellular targets of the HTLV-I oncoprotein Tax, we identified TXBP181, which we characterized as the human homolog of yeast mitotic checkpoint MAD1 protein. Evidence supporting TXBP181 as HsMAD1 includes sequence conservation with yeast MAD1, hyperphosphorylation during S/G2/M phases and upon treatment of cells with nocodazole, and binding ... More
Functional Domains Present in the Mycobacterial Hemagglutinin, HBHA
AuthorsGiovanni Delogu and Michael J. Brennan*
JournalJ Bacteriol
PubMed ID10601202
'Identification and characterization of mycobacterial adhesins and complementary hostreceptors required for colonization and dissemination of mycobacteria in host tissues areneeded for a more complete understanding of the pathogenesis of diseases caused by thesebacteria and for the development of effective vaccines. Previous investigations havedemonstrated that a 28-kDa heparin-binding mycobacterial surface protein, ... More
Characterization of the Pathway-Specific Positive Transcriptional Regulator for ActinorhodinBiosynthesis in Streptomyces coelicolor A3(2) as a DNA-Binding Protein
AuthorsPaloma Arias, Miguel A. Fernßndez-Moreno, and Francisco Malpartida*
JournalJ Bacteriol
PubMed ID10559161
'The ActII-ORF4 protein has been characterized as a DNA-binding protein that positively regulates thetranscription of the actinorhodin biosynthetic genes. The target regions for the ActII-ORF4 protein werelocated within the act cluster. These regions, at high copy number, generate a nonproducer strain by invivo titration of the regulator. The mutant phenotype ... More
When an ATPase is not an ATPase: at low temperatures the C-terminal domain of the ABC transporter CvaB is a GTPase.
AuthorsZhong X, Tai PC
JournalJ Bacteriol
PubMed ID9515899
'The ATP-binding cassette (ABC) transporters belong to a large superfamily of proteins which share a common function and a common nucleotide-binding domain. The CvaB protein from Escherichia coli is a member of the bacterial ABC exporter subfamily and is essential for the export of the peptide antibiotic colicin V. Here ... More
Site-directed mutagenesis of benzalacetone synthase. The role of the Phe215 in plant type III polyketide synthases.
AuthorsAbe I, Sano Y, Takahashi Y, Noguchi H,
JournalJ Biol Chem
PubMed ID12724310
'Benzalacetone synthase (BAS) and chalcone synthase (CHS) are plant-specific type III polyketide synthases (PKSs) that share approximately 70% amino acid sequence identity. BAS catalyzes a one-step decarboxylative condensation of 4-coumaroyl-CoA with malonyl-CoA to produce a diketide benzalacetone, whereas CHS performs sequential condensations with three malonyl-CoA to generate a tetraketide chalcone. ... More
Disarming the mustard oil bomb.
Authors Ratzka Andreas; Vogel Heiko; Kliebenstein Daniel J; Mitchell-Olds Thomas; Kroymann Juergen;
JournalProc Natl Acad Sci U S A
PubMed ID12161563
Plants are attacked by a broad array of herbivores and pathogens. In response, plants deploy an arsenal of defensive traits. In Brassicaceae, the glucosinolate-myrosinase complex is a sophisticated two-component system to ward off opponents. However, this so-called  ... More
An endoplasmic reticulum protein implicated in chaperoning peptides to major histocompatibility of class I is an aminopeptidase.
Authors Ménoret A; Li Z; Niswonger M L; Altmeyer A; Srivastava P K;
JournalJ Biol Chem
PubMed ID11397801
gp96, an abundant peptide-binding chaperone of the lumen of the endoplasmic reticulum and an acceptor of peptides transported into the endoplasmic reticulum through transporter associated with antigen processing, is shown to be an aminopeptidase. gp96 can trim an amino-terminal extended 19-mer precursor of the K(b)-binding VSV8 epitope for recognition by ... More
A mammalian protein with specific demethylase activity for mCpG DNA [see comments]
AuthorsBhattacharya SK, Ramchandani S, Cervoni N, Szyf M
JournalNature
PubMed ID10050851
DNA-methylation patterns are important for regulating genome functions, and are determined by the enzymatic processes of methylation and demethylation. The demethylating enzyme has now been identified: a mammalian complementary DNA encodes a methyl-CpG-binding domain, bears a demethylase activity that transforms methylated cytosine bases to cytosine, and demethylates a plasmid when ... More
Immune hyperactivation of HIV-1-infected T cells mediated by Tat and the CD28 pathway.
AuthorsOtt M, Emiliani S, Van Lint C, Herbein G, Lovett J, Chirmule N, McCloskey T, Pahwa S, Verdin E
JournalScience
PubMed ID9045614
Human immunodeficiency virus-type 1 (HIV-1) infection is characterized by a chronic state of immune hyperactivation in patients. Infection of human peripheral blood lymphocytes with HIV-1 in vitro resulted in increased interleukin-2 (IL-2) secretion in response to T cell activation via the CD3 and CD28 receptors. Expression of the HIV-1 transactivator ... More
NMR structure and mutagenesis of the N-terminal Dbl homology domain of the nucleotide exchange factor Trio.
AuthorsLiu X, Wang H, Eberstadt M, Schnuchel A, Olejniczak ET, Meadows RP, Schkeryantz JM, Janowick DA, Harlan JE, Harris EA, Staunton DE, Fesik SW
JournalCell
PubMed ID9790533
Guanine nucleotide exchange factors for the Rho family of GTPases contain a Dbl homology (DH) domain responsible for catalysis and a pleckstrin homology (PH) domain whose function is unknown. Here we describe the solution structure of the N-terminal DH domain of Trio that catalyzes nucleotide exchange for Rac1. The all-alpha-helical ... More
Pigment Epithelium-Derived Factor: A Potent Inhibitor of Angiogenesis
AuthorsD. W. Dawson, O. V. Volpert, P. Gillis, S. E. Crawford, H.-J. Xu, W. Benedict, and N. P. Bouck
JournalScience
PubMed ID10398599
In the absence of disease, the vasculature of the mammalian eyeis quiescent, in part because of the action of angiogenicinhibitors that prevent vessels from invading the cornea andvitreous. Here, an inhibitor responsible for the avascularity ofthese ocular compartments is identified as pigmentepithelium-derived factor (PEDF), a protein previously shownto have neurotrophic ... More
The Nucleoporin RanBP2 Has SUMO1 E3 Ligase Activity.
Authors Pichler Andrea; Gast Andreas; Seeler Jacob S; Dejean Anne; Melchior Frauke;
JournalCell
PubMed ID11792325
Posttranslational modification with SUMO1 regulates protein/protein interactions, localization, and stability. SUMOylation requires the E1 enzyme Aos1/Uba2 and the E2 enzyme Ubc9. A family of E3-like factors, PIAS proteins, was discovered recently. Here we show that the nucleoporin RanBP2/Nup358 also has SUMO1 E3-like activity. RanBP2 directly interacts with the E2 enzyme ... More
Defective CD95/APO-1/Fas signal complex formation in the human autoimmune lymphoproliferative syndrome, type Ia.
AuthorsMartin DA, Zheng L, Siegel RM, Huang B, Fisher GH, Wang J, Jackson CE, Puck JM, Dale J, Straus SE, Peter ME, Krammer PH, Fesik S, Lenardo MJ
JournalProc Natl Acad Sci U S A
PubMed ID10200300
Heterozygous mutations in the CD95 (APO-1/Fas) receptor occur in most individuals with autoimmune lymphoproliferative syndrome (ALPS) and dominantly interfere with apoptosis by an unknown mechanism. We show that local or global alterations in the structure of the cytoplasmic death domain from nine independent ALPS CD95 death-domain mutations result in a ... More
Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline.
Authors Lesley Scott A; Kuhn Peter; Godzik Adam; Deacon Ashley M; Mathews Irimpan; Kreusch Andreas; Spraggon Glen; Klock Heath E; McMullan Daniel; Shin Tanya; Vincent Juli; Robb Alyssa; Brinen Linda S; Miller Mitchell D; McPhillips Timothy M; Miller Mark A; Scheibe Daniel; Canaves Jaume M; Guda Chittibabu; Jaroszewski Lukasz; Selby Thomas L; Elsliger Marc-Andre; Wooley John; Taylor Susan S; Hodgson Keith O; Wilson Ian A; Schultz Peter G; Stevens Raymond C;
JournalProc Natl Acad Sci U S A
PubMed ID12193646
Structural genomics is emerging as a principal approach to define protein structure-function relationships. To apply this approach on a genomic scale, novel methods and technologies must be developed to determine large numbers of structures. We describe the design and implementation of a high-throughput structural genomics pipeline and its application to ... More
ProNGF induces p75-mediated death of oligodendrocytes following spinal cord injury.
AuthorsBeattie MS, Harrington AW, Lee R, Kim JY, Boyce SL, Longo FM, Bresnahan JC, Hempstead BL, Yoon SO,
JournalNeuron
PubMed ID12408842
The neurotrophin receptor p75 is induced by various injuries to the nervous system, but its role after injury has remained unclear. Here, we report that p75 is required for the death of oligodendrocytes following spinal cord injury, and its action is mediated mainly by proNGF. Oligodendrocytes undergoing apoptosis expressed p75, ... More
Functionally important calmodulin-binding sites in both NH2- and COOH-terminal regions of the cone photoreceptor cyclic nucleotide-gated channel CNGB3 subunit.
AuthorsPeng C, Rich ED, Thor CA, Varnum MD,
JournalJ Biol Chem
PubMed ID12730238
Whereas an important aspect of sensory adaptation in rod photoreceptors and olfactory receptor neurons is thought to be the regulation of cyclic nucleotide-gated (CNG) channel activity by calcium-calmodulin (Ca2+-CaM), it is not clear that cone photoreceptor CNG channels are similarly modulated. Cone CNG channels are composed of at least two ... More
Purification and characterization of a human RNA adenosine deaminase for glutamate receptor B pre-mRNA editing.
AuthorsYang JH, Sklar P, Axel R, Maniatis T
JournalProc Natl Acad Sci U S A
PubMed ID9113993
The glutamate receptor subunit B (GluR-B) pre-mRNA is edited at two adenosine residues, resulting in amino acid changes that alter the electrophysiologic properties of the glutamate receptor. Previous studies showed that these amino acid changes are due to adenosine to inosine conversions in two codons resulting from adenosine deamination. Here, ... More
Axonal localisation of the CAM-like tyrosine phosphatase CRYP alpha: a signalling molecule of embryonic growth cones.
AuthorsStoker AW, Gehrig B, Haj F, Bay BH
JournalDevelopment
PubMed ID7600997
Migrating embryonic growth cones require multiple, membrane-associated signalling molecules to monitor and respond to guidance cues. Here we present the first evidence that vertebrate cell adhesion molecule-like protein tyrosine phosphatases are likely to be components of this signalling system. CRYP alpha, the gene for an avian cell adhesion molecule-like phosphatase, ... More
Studies on the interactions between human replication factor C and human proliferating cell nuclear antigen.
AuthorsZhang G, Gibbs E, Kelman Z, O'Donnell M, Hurwitz J
JournalProc Natl Acad Sci U S A
PubMed ID10051561
Proliferating cell nuclear antigen (PCNA) is a processivity factor required for DNA polymerase delta (or epsilon)-catalyzed DNA synthesis. When loaded onto primed DNA templates by replication factor C (RFC), PCNA acts to tether the polymerase to DNA, resulting in processive DNA chain elongation. In this report, we describe the identification ... More
Asp333, Asp495, and His523 form the catalytic triad of rat soluble epoxide hydrolase.
AuthorsArand M, Wagner H, Oesch F
JournalJ Biol Chem
PubMed ID8626766
On the basis of the sequence similarity between mammalian epoxide hydrolases and bacterial haloalkane dehalogenase reported earlier (Arand, M., Grant, D. F., Beetham, J. K., Friedberg, T., Oesch, F., and Hammock, B. D. (1994) FEBS Lett. 338, 251-256; Beetham, J. K., Grant, D., Arand, M., Garbarino, J., Kiyosue, T., Pinot, ... More
Proapoptotic BH3-only Bcl-2 family members induce cytochrome c release, but not mitochondrial membrane potential loss, and do not directly modulate voltage-dependent anion channel activity.
AuthorsShimizu S, Tsujimoto Y
JournalProc Natl Acad Sci U S A
PubMed ID10639121
Through direct interaction with the voltage-dependent anion channel (VDAC), proapoptotic Bcl-2 family members such as Bax and Bak induce apoptogenic mitochondrial cytochrome c release and membrane potential (Deltapsi) loss in isolated mitochondria. Using isolated mitochondria, we showed that Bid and Bik, BH3-only proteins from the Bcl-2 family, induced cytochrome c ... More
Distinct HOX N-terminal arm residues are responsible for specificity of DNA recognition by HOX monomers and HOX.PBX heterodimers.
AuthorsPhelan ML, Featherstone MS
JournalJ Biol Chem
PubMed ID9079695
Dimerization with extradenticle or PBX homeoproteins dramatically improves DNA binding by HOX transcription factors, indicating that recognition by such complexes is important for HOX specificity. For HOX monomeric binding, a major determinant of specificity is the flexible N- terminal arm. It makes base-specific contacts via the minor groove, including one ... More
The RecD subunit of the RecBCD enzyme from Escherichia coli is a single- stranded DNA-dependent ATPase.
AuthorsChen HW, Ruan B, Yu M, Wang Jd, Julin DA
JournalJ Biol Chem
PubMed ID9092551
We have expressed the RecD subunit of the RecBCD enzyme from Escherichia coli as a fusion protein with a 31-amino acid NH2-terminal extension including 6 consecutive histidine residues (HisRecD). The overexpressed fusion protein can be purified in urea-denatured form by metal chelate affinity chromatography. The mixture of renatured HisRecD protein ... More
The mitochondrial protein hFis1 regulates mitochondrial fission in mammalian cells through an interaction with the dynamin-like protein DLP1.
AuthorsYoon Y, Krueger EW, Oswald BJ, McNiven MA,
JournalMol Cell Biol
PubMed ID12861026
The yeast protein Fis1p has been shown to participate in mitochondrial fission mediated by the dynamin-related protein Dnm1p. In mammalian cells, the dynamin-like protein DLP1/Drp1 functions as a mitochondrial fission protein, but the mechanisms by which DLP1/Drp1 and the mitochondrial membrane interact during the fission process are undefined. In this ... More
Glycogen synthase kinase 3 is an insulin-regulated C/EBPalpha kinase
AuthorsRoss SE, Erickson RL, Hemati N, MacDougald OA
JournalMol Cell Biol
PubMed ID10567568
CCAAT/enhancer binding protein alpha (C/EBPalpha) is a transcription factor involved in creating and maintaining the adipocyte phenotype. We have shown previously that insulin stimulates dephosphorylation of C/EBPalpha in 3T3-L1 adipocytes. Studies to identify the insulin-sensitive sites of phosphorylation reveal that a C/EBPalpha peptide (amino acids H215 to K250) is phosphorylated ... More
Tryptophan fluorescence reports nucleotide-induced conformational changes in a domain of the ArsA ATPase.
AuthorsZhou T, Rosen BP
JournalJ Biol Chem
PubMed ID9242630
The ars operon of plasmid R773 encodes an ATP-dependent extrusion pump for arsenite and antimonite in Escherichia coli. The ArsA ATPase is the catalytic subunit of the pump protein, with two nucleotide binding consensus sequences, one in the NH2-terminal half and one in the COOH- terminal half of the protein. ... More
The high affinity heparin-binding domain and the V region of fibronectin mediate invasion of human oral squamous cell carcinoma cells in vitro.
AuthorsKapila YL, Niu J, Johnson PW
JournalJ Biol Chem
PubMed ID9228073
Fibronectin is an extracellular matrix molecule composed of repeating subunits that create functional domains. These domains contain multiple binding sites for heparin and for various cell-surface receptors that modulate cell function. To examine the role that the high affinity heparin-binding region and the alternatively spliced V region of fibronectin play ... More
Cloning of Chinese hamster DNA topoisomerase I cDNA and identification of a single point mutation responsible for camptothecin resistance.
AuthorsTanizawa A, Beitrand R, Kohlhagen G, Tabuchi A, Jenkins J, Pommier Y
JournalJ Biol Chem
PubMed ID8244980
A camptothecin-resistant (DC3F/C-10) Chinese hamster cell line that contains a catalytically altered and camptothecin (CPT)-resistant DNA topoisomerase I (top 1) (Tanizawa, A., and Pommier, Y. (1992) Cancer Res. 52, 1848-1854) and the parent cell line (DC3F) were used to compare top 1 mRNAs and cDNAs. Northern blot analysis showed a ... More
Cooperative interaction between GATA-4 and GATA-6 regulates myocardial gene expression.
AuthorsCharron F, Paradis P, Bronchain O, Nemer G, Nemer M
JournalMol Cell Biol
PubMed ID10330176
Two members of the GATA family of transcription factors, GATA-4 and GATA-6, are expressed in the developing and postnatal myocardium and are equally potent transactivators of several cardiac promoters. However, several in vitro and in vivo lines of evidence suggest distinct roles for the two factors in the heart. Since ... More
5' phospholipid phosphatase SHIP-2 causes protein kinase B inactivation and cell cycle arrest in glioblastoma cells.
AuthorsTaylor V, Wong M, Brandts C, Reilly L, Dean NM, Cowsert LM, Moodie S, Stokoe D
JournalMol Cell Biol
PubMed ID10958682
The tumor suppressor protein PTEN is mutated in glioblastoma multiform brain tumors, resulting in deregulated signaling through the phosphoinositide 3-kinase (PI3K)-protein kinase B (PKB) pathway, which is critical for maintaining proliferation and survival. We have examined the relative roles of the two major phospholipid products of PI3K activity, phosphatidylinositol 3,4-biphosphate ... More
Heme oxygenase-2 is a hemoprotein and binds heme through heme regulatory motifs that are not involved in heme catalysis.
AuthorsMcCoubrey WK Jr, Huang TJ, Maines MD
JournalJ Biol Chem
PubMed ID9139709
The heme oxygenase (HO) system degrades heme to biliverdin and CO and releases chelated iron. In the primary sequence of the constitutive form, HO-2, there are three potential heme binding sites: two heme regulatory motifs (HRMs) with the absolutely conserved Cys-Pro pair, and a conserved 24-residue heme catalytic pocket with ... More
Characterization of the coronavirus mouse hepatitis virus strain A59 small membrane protein E.
AuthorsRaamsman MJ, Locker JK, de Hooge A, de Vries AA, Griffiths G, Vennema H, Rottier PJ
JournalJ Virol
PubMed ID10666264
The small envelope (E) protein has recently been shown to play an essential role in the assembly of coronaviruses. Expression studies revealed that for formation of the viral envelope, actually only the E protein and the membrane (M) protein are required. Since little is known about this generally low-abundance virion ... More
Structure and ligand of a histone acetyltransferase bromodomain.
AuthorsDhalluin C, Carlson JE, Zeng L, He C, Aggarwal AK, Zhou MM
JournalNature
PubMed ID10365964
Histone acetylation is important in chromatin remodelling and gene activation. Nearly all known histone-acetyltransferase (HAT)-associated transcriptional co-activators contain bromodomains, which are approximately 110-amino-acid modules found in many chromatin-associated proteins. Despite the wide occurrence of these bromodomains, their three-dimensional structure and binding partners remain unknown. Here we report the solution structure ... More
Analysis of Schizosaccharomyces pombe mediator reveals a set of essential subunits conserved between yeast and metazoan cells.
Authors Spåhr H; Samuelsen C O; Baraznenok V; Ernest I; Huylebroeck D; Remacle J E; Samuelsson T; Kieselbach T; Holmberg S; Gustafsson C M;
JournalProc Natl Acad Sci U S A
PubMed ID11572939
With the identification of eight new polypeptides, we here complete the subunit characterization of the Schizosaccharomyces pombe RNA polymerase II holoenzyme. The complex contains homologs to all 10 essential gene products present in the Saccharomyces cerevisiae Mediator, but lacks clear homologs to any of the 10 S. cerevisiae components encoded ... More
A quorum sensing-associated virulence gene of Pseudomonas aeruginosa encodes a LysR-like transcription regulator with a unique self-regulatory mechanism.
Authors Cao H; Krishnan G; Goumnerov B; Tsongalis J; Tompkins R; Rahme L G;
JournalProc Natl Acad Sci U S A
PubMed ID11724939
The human opportunistic pathogen Pseudomonas aeruginosa strain PA14 infects both plants and animals. Previously, using plants to screen directly for P. aeruginosa virulence-attenuated mutants, we identified a locus, pho34B12, relevant in mammalian pathogenesis. Here, nonsense point mutations in the two opposing ORFs identified in the pho34B12 locus revealed that one ... More
U box proteins as a new family of ubiquitin-protein ligases.
Authors Hatakeyama S; Yada M; Matsumoto M; Ishida N; Nakayama K I;
JournalJ Biol Chem
PubMed ID11435423
The U box is a domain of approximately 70 amino acids that is present in proteins from yeast to humans. The prototype U box protein, yeast Ufd2, was identified as a ubiquitin chain assembly factor that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin-protein ligase ... More
An endoplasmic reticulum stress-specific caspase cascade in apoptosis. Cytochrome c-independent activation of caspase-9 by caspase-12.
Authors Morishima Nobuhiro; Nakanishi Keiko; Takenouchi Hiromi; Shibata Takehiko; Yasuhiko Yukuto;
JournalJ Biol Chem
PubMed ID12097332
Activation of caspase-12 from procaspase-12 is specifically induced by insult to the endoplasmic reticulum (ER) (Nakagawa, T., Zhu, H., Morishima, N., Li, E., Xu, J., Yankner, B. A., and Yuan, J. (2000) Nature 403, 98-103), yet the functional consequences of caspase-12 activation have been unclear. We have shown that recombinant ... More
Identification, molecular cloning, and characterization of a novel GABAA receptor-associated protein, GRIF-1.
AuthorsBeck M, Brickley K, Wilkinson HL, Sharma S, Smith M, Chazot PL, Pollard S, Stephenson FA.
JournalJ Biol Chem
PubMed ID12034717
A novel 913-amino acid protein, gamma-aminobutyric acid type A (GABA(A)) receptor interacting factor-1 (GRIF-1), has been cloned and identified as a GABA(A) receptor-associated protein by virtue of its specific interaction with the GABA(A) receptor beta 2 subunit intracellular loop in a yeast two-hybrid assay. GRIF-1 has no homology with proteins ... More